3,636 results

1. pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry

   Plasmid

   #159631

   Purpose

   expresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoter

   Depositor

   Andrew Allen, Ross Bathgate

   Article

   Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

   Insert

   Ast (AstA Fly)
   

   Use

   Lentiviral

   Tags

   HA, IRES, mCherry, and neurophysin-1

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. OA-1045B

   Plasmid

   #125004

   Purpose

   Expresses gRNAs targeting hid, eve, and hedgehog

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   eve gRNA (eve Fly)
   

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. OA-1045D

   Plasmid

   #125006

   Purpose

   Expresses gRNAs targeting hedgehog and wingless

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   wg gRNA (wg Fly)
   

   Expression

   Insect

   Promoter

   u6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. OA-1045E

   Plasmid

   #125007

   Purpose

   Expresses tRNA-flanked gRNAs targeting hid, eve, and hedgehog

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   eve gRNA (eve Fly)
   

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. OA-1045A

   Plasmid

   #125003

   Purpose

   Expresses gRNAs targeting hid and eve

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   eve gRNA (eve Fly)
   

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hy_pMT Flag-Hel25E MUT

   Plasmid

   #110122

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   4 mutations (KKLN motif changed to RSFS)

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. OA-1050I (notch)

   Plasmid

   #132421

   Purpose

   express arrays of gRNA targeting Notch under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   notch gRNA array
   

   Expression

   Insect

   Promoter

   U6-3

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. OA-1050K (firefly luciferase)

   Plasmid

   #132422

   Purpose

   express arrays of gRNA targeting Firefly Luciferase under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   firefly Luciferase gRNA array
   

   Expression

   Insect

   Promoter

   U6-3

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. OA-1050U (cinnabar)

   Plasmid

   #132423

   Purpose

   express arrays of gRNA targeting Cinnabar under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   cinnabar gRNA array
   

   Expression

   Insect

   Promoter

   U6-3

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. OA-1050V (wingless)

    Plasmid

    #132424

    Purpose

    express arrays of gRNA targeting Wingless under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    wingless gRNA array
    

    Expression

    Insect

    Promoter

    U6-3

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. OA-1050Z4 (yellow)

    Plasmid

    #132425

    Purpose

    express arrays of gRNA targeting Yellow under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    yellow gRNA array
    

    Expression

    Insect

    Promoter

    U6-3

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. OA-1052B (Ubiq-Fluc_Ubiq-Rluc)

    Plasmid

    #132426

    Purpose

    Express Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Inserts

    Ubiq promoter
    firefly luciferase
    T2A-eGFP
    Renilla Luciferase-SV40-P10
    reversed ubiq promoter
    

    Expression

    Insect

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. OA-1050L (UASt-CasRx)

    Plasmid

    #132418

    Purpose

    Express CasRx under UASt promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Inserts

    CasRx
    UASt+hsp70
    

    Expression

    Insect

    Promoter

    UASt

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Drosophila Nedd2-like caspase (DRONC)

    Plasmid

    #157778

    Purpose

    E. coli expression vector pET23 with C-term 6xHis tag

    Depositor

    Guy Salvesen

    Article

    Snipas et al Cell Death Differ. 2008 May;15(5):938-45

    Insert

    Drosophila Nedd2-like caspase (DRONC) (Dronc Fly)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Opie2-PuroR[dsxM]

    Plasmid

    #131616

    Purpose

    Expresses a functional PuroR only in males and dsRed in both genders.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Inserts

    PuroR[dsxM] (dsx Fly)
    dsRed
    

    Expression

    Insect

    Promoter

    Opie2 and hr5Ie1

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Opie2-NeoR[TraF]

    Plasmid

    #131617

    Purpose

    Expresses a functional NeoR only in females and dsRed in both genders.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Insert

    NeoR[traF]
    

    Expression

    Insect

    Promoter

    Opie2

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFast-dCry-V5His

    Plasmid

    #136240

    Purpose

    Protein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tags

    Depositor

    Aziz Sancar

    Article

    Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

    Insert

    DmCry
    

    Tags

    His and V5

    Expression

    Insect

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28P_dCrk

    Plasmid

    #131145

    Purpose

    Expresses 6XHis::dCrk (based on crk isoform RA).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Tags

    6XHis

    Expression

    Bacterial

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHD-dsRed-attP_dCrk_flankingHRs

    Plasmid

    #131141

    Purpose

    Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Inserts

    Crk Upstream Homology Region (Crk Fly)
    Crk Downstream Homology Region (Crk Fly)
    

    Use

    CRISPR and Cre/Lox; Homology directed repair temp…

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-chiRNA_dCrkgRNA1

    Plasmid

    #131139

    Purpose

    Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    dCrk gRNA1 (Crk Fly)
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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