We narrowed to 3,069 results for: FRI

1. p-preSufI(IAC)

   Plasmid

   #168516

   Purpose

   Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

   Depositor

   Siegfried Musser

   Article

   Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

   Insert

   pre-SufI(IAC)
   

   Use

   Tags

   6xHisC tag and HSV tag

   Expression

   Bacterial

   Mutation

   C17I and C295A

   Promoter

   T7

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGAD424-hSyntenin2a

   Plasmid

   #89426

   Purpose

   Two-hybrid system

   Depositor

   Fritz Rathjen

   Article

   Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

   Insert

   SDCBP2 (SDCBP2 Human)
   

   Use

   Tags

   Gal4 AD

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Fragment1 with TLS2

   Plasmid

   #196983

   Purpose

   Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Intermediate fragment for assembly of gRNA-TLS2 construct
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. gRNA NIA Null

   Plasmid

   #198728

   Purpose

   Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AtNIA1 gRNAs
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pU6-26, pU6-29

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-hL1

   Plasmid

   #89411

   Purpose

   mammalian expression of hL1

   Depositor

   Fritz Rathjen

   Article

   De Angelis et al EMBO J. 1999 Sep 1. 18(17):4744-53.

   Insert

   L1CAM (L1CAM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21-Flavidin

   Plasmid

   #89881

   Purpose

   Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling

   Depositor

   Mark Howarth

   Article

   Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

   Insert

   Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
   

   Use

   Tags

   6 glutamate tag (C terminal on insert)

   Expression

   Bacterial

   Mutation

   K80R, K121R, K132R, K134R

   Promoter

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. EC71173

   Plasmid

   #154061

   Purpose

   Level 1, Position 2 Golden Gate vector. HspHv17::Cre-U5-Cre

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   Cre recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Introduction of U5 intron sequences, to prevent p…

   Promoter

   HvHSP17

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393

   Plasmid

   #216380

   Purpose

   Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Frizzled-4 (Fzd4 Mouse)
   

   Use

   Baculovirus

   Tags

   3C-splitGFP1to10opt-ETVV and HA signal sequence-F…

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG Fzd7 GFP

   Plasmid

   #66106

   Purpose

   expression of chicken Fzd7 GFP

   Depositor

   Martin Scaal, Felicitas Proels

   Article

   et al Development. 2015 Feb 15;142(4):665-71.

   Insert

   Fzd7 (FZD7 Chicken)
   

   Use

   Chicken expression

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP-hSyntenin2b

    Plasmid

    #89437

    Purpose

    mammalian expression of EGFP tagged human Syntenin2b

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP2 (SDCBP2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    *see below

    Promoter

    CMV

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. TET-pLKO-neo CXCR4-sh3

    Plasmid

    #163743

    Purpose

    Lentivirus for inducible knockdown of CXCR4 (human)

    Depositor

    Roland Friedel

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. TET-pLKO-neo CXCR4-sh1

    Plasmid

    #163741

    Purpose

    Lentivirus for inducible knockdown of CXCR4 (human)

    Depositor

    Roland Friedel

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TET-pLKO-neo CXCR4-sh2

    Plasmid

    #163742

    Purpose

    Lentivirus for inducible knockdown of CXCR4 (human)

    Depositor

    Roland Friedel

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. mFzd4CRDL(42-203)-6xHis_pAcGP67a

    Plasmid

    #216379

    Purpose

    Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells

    Depositor

    William Weis

    Article

    Bruguera et al bioRxiv 2024.02.03.578714

    Insert

    Frizzled-4 (Fzd4 Mouse)
    

    Use

    Baculovirus

    Tags

    6xHis and GP64 signal sequence

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. tetO-Fzd1-IRES-tdTomato (TET006)

    Plasmid

    #160510

    Purpose

    This plasmid expresses Fzd1 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

    Depositor

    C. Ron Yu

    Article

    Wu et al bioRxiv 2022

    Insert

    Fzd1 (Fzd1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. DT048A

    Plasmid

    #159409

    Purpose

    Constitutive LuxR expression, controlled under J23101 E.coli promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23101-LuxR-pr.LuxR-PFO
    

    Use

    Synthetic Biology

    Tags

    FLAG/FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    J23101 and pLux promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. DT046A

    Plasmid

    #159407

    Purpose

    Constitutive LuxR expression, controlled under E.coli tet promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.tet-LuxR-pr.LuxR-PFO
    

    Use

    Synthetic Biology

    Tags

    FLAG/FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    tet and pLux promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. gRNA NIA TLS2

    Plasmid

    #196981

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS2 tags
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pU6-26, pU6-29

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. gRNA NIA TLS1

    Plasmid

    #196980

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS1 tags
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pU6-26, pU6-29

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-BLM

    Plasmid

    #80070

    Purpose

    Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)

    Depositor

    Nathan Ellis

    Article

    Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98

    Insert

    BLM (BLM Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    If correct ID, the cDNA is from HeLa and has char…

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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