We narrowed to 5,479 results for: KRAS

1. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

   Plasmid

   #212805

   Purpose

   Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
   

   Expression

   Mammalian

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET-HypaR-SpCas9-NLS-6xHis

   Plasmid

   #207387

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HypaR-SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R661A, N692A, M694A, Q695A, H698A

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-SpCas9-HF1-NLS-6xHis

   Plasmid

   #207382

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. B-HiFi SpCas9

   Plasmid

   #207362

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HiFi SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R691A, amino acids 1005-1013 replaced with two gl…

   Promoter

   CBh

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-HeFSpCas9-NLS-6xHis

   Plasmid

   #207391

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-B-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207378

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A, amino acids 1005-1013 replaced with two gl…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PET-Blackjack SpCas9-NLS-6xHis

   Plasmid

   #207375

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Blackjack SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   amino acids 1005-1013 replaced with two glycine

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNIC28-HiBIT-WDR5

   Plasmid

   #200883

   Purpose

   Bacterial expression of N-terminal HiBIT-tagged human WDR5 protein

   Depositor

   Stefan Knapp

   Article

   Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

   Insert

   HiBIT-WDR5 (WDR5 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRps9-WT-IRES-GFP-HygR

   Plasmid

   #190271

   Purpose

   Express RPS9 WT mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.

   Depositor

   Erik Böttger

   Article

   Brilkova et al Cell Rep. 2022 Sep 27;40(13):111433. doi

   Insert

   ribosomal protein RPS9
   

   Tags

   IRES-eGFP

   Expression

   Mammalian

   Promoter

   CAGGS-CMV

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRps9-D95N-IRES-GFP-HygR

    Plasmid

    #190272

    Purpose

    Express RPS9 D95N-mutant mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.

    Depositor

    Erik Böttger

    Article

    Brilkova et al Cell Rep. 2022 Sep 27;40(13):111433. doi

    Insert

    ribosomal protein RPS9
    

    Tags

    IRES-eGFP

    Expression

    Mammalian

    Mutation

    D95N

    Promoter

    CAGGS-CMV

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAT15541_dCBE-SuperFi-Cas9

    Plasmid

    #184371

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dCBE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAT15543_dABE-SuperFi-Cas9

    Plasmid

    #184373

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dABE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    FLAG, SV40 NLS, and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAT15545_dABE8e-SuperFi-Cas9

    Plasmid

    #184375

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dABE8e-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b p97-His D592Amb

    Plasmid

    #169022

    Purpose

    Expresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteria

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    D592TAG

    Promoter

    T7

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. ARB366

    Plasmid

    #124049

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CMV-TIRAP-cGASΔN-HA

    Plasmid

    #130921

    Purpose

    Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    TIRAP-ΔNcGAS
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    cGAS N terminus (aa1-159) replaced with the N ter…

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-6P-1 p37delta2-81

    Plasmid

    #113502

    Purpose

    Bacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    delta 2-81

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFL His-I3

    Plasmid

    #113513

    Purpose

    Expression of His-I3 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Tags

    6*His

    Expression

    Insect

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Thy-C73A-HA

    Plasmid

    #111542

    Purpose

    For expression of human KCNB1 in mouse brain

    Depositor

    Federico Sesti

    Article

    Yu et al J Neurosci. 2016 Oct 26;36(43):11084-110

    Insert

    thy1.2-C73A-HA (KCNB1 Human)
    

    Use

    Mouse Targeting

    Tags

    HA

    Mutation

    C73A

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

    Plasmid

    #58490

    Purpose

    Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3

    Insert

    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Aspartic Acid 95 to Glutamine

    Promoter

    Ubiquitin

    Available Since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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