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Showing: 4081 - 4110 of 4159 results

  1. pBJG1 T7-8xHis-OGT 5N5A

    Plasmid
    #154280
    Purpose
    Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; bact…
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154280-plasmid-map-sequence-id-304840

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  4. Pdpy-30-miniCAFE

    Plasmid
    #170115
    Purpose
    Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.
    Depositor
    Zhili Rong
    Article
    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617
    Insert
    MiniCAFE
    Use
    CRISPR
    Tags
    Expression
    Mutation
    D8A, HNH-truncation (Δ495–609 aa) in CjCas9
    Promoter
    Dpy-30
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170115-plasmid-map-sequence-id-336055

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  11. pINQ-ON10

    Plasmid
    #198689
    Purpose
    Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)
    Depositor
    Gualberto González Sapienza
    Article
    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d
    Insert
    anti-SARS-CoV-2 Nucleocapsid nanobody ON10
    Use
    Tags
    6xHis tag, HA tag, OmpA signal peptide, and Ribos…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    198689-plasmid-map-sequence-id-402802

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  13. hzHDAC10

    Plasmid
    #225542
    Purpose
    Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.
    Depositor
    David Christianson
    Article
    Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21
    Insert
    Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
    Use
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Mutation
    Mutated A24E and D94A
    Promoter
    T7
    Available since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225542-plasmid-map-sequence-id-448447

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  28. FCM101

    Plasmid
    #194298
    Purpose
    E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6
    Depositor
    Shohei Koide
    Article
    Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi:
    Insert
    FCM101
    Use
    Tags
    His tag, FLAG tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    194298-plasmid-map-sequence-id-381074

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Showing: 4081 - 4110 of 4159 results