We narrowed to 4,119 results for: spl

Showing: 2721 - 2740 of 4119 results

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SPELL-ITSN1(DH-PH) Component 1

Plasmid

#170491

Purpose

SPELL ITSN1(DH-PH) component 1. mCherry-ITSN(N-lobe) fused to iFKBP. Split at loop 4 of DH domain.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

mCherry, ITSN(N-lobe), iFKBP

Expression

Mammalian

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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SPELL-ITSN1(DH-PH) Component 2

Plasmid

#170492

Purpose

SPELL ITSN1(DH-PH) component 2. myc-FRB-ITSN(C-lobe). Split at loop 4 of DH domain.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

myc, ITSN1(C-lobe), FRB

Expression

Mammalian

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMV23

Plasmid

#128887

Purpose

Position 4 module- STOP (stop codon)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

STOP

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMV10

Plasmid

#128892

Purpose

Position 6 module- URA3

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

URA3 (URA3 Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMV5

Plasmid

#128886

Purpose

Position 4 module- CLN2-PEST (Degron)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

CLN2-PEST

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI125

Plasmid

#126065

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

YFP1-10

Use

Synthetic Biology

Tags

linker (GTGGGGS)

Mutation

T205Y

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pVP2del50

Plasmid

#125185

Purpose

Expresses the VP2del50 truncation mutant protein derived from AAV2 VP2

Depositor

Junghae Suh

Article

Thadani et al ACS Nano. 2018 Feb 27;12(2):1445-1454. d

Insert

hexahistidine tag

Expression

Mammalian

Mutation

Inserted start codon and hexahistidine tag at 2697

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pVP2del30

Plasmid

#125184

Purpose

Expresses the VP2del30 truncation mutant protein derived from AAV2 VP2

Depositor

Junghae Suh

Article

Thadani et al ACS Nano. 2018 Feb 27;12(2):1445-1454. d

Insert

hexahistidine tag

Expression

Mammalian

Mutation

Inserted start codon and hexahistidine tag at 2637

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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sZF7

Plasmid

#43838

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF7

Tags

HA Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
sZF11

Plasmid

#43842

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF11

Tags

HA Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pRF TpC RE1

Plasmid

#171040

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE1

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE1

Use

Luciferase

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pRF TpC RE3

Plasmid

#171042

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE3

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE3

Use

Luciferase

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pRF TpC RE5

Plasmid

#171044

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE5

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE5

Use

Luciferase

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pT7RNAPNev-NB1

Plasmid

#216336

Purpose

Arabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-eGFP nanobody NB1

Depositor

Mark Styczynski

Article

McSweeney et al Sci Adv. 2025 Feb 21;11(8):eado6280. doi

Insert

T7RNAPN-NB1 fusion

Expression

Bacterial

Available Since

July 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pSS2 - pBait-1xMS2-7GC[empty]-TtrpA

Plasmid

#222406

Purpose

arabinose-induced synthesis of an RNA containing an MS2 hairpin, 7GC clamp and trpA terminator. Used as a three-hybrid control. For cloning purposes, see plasmid #222404.

Depositor

Katherine Berry

Article

Nguyen et al Sci Rep. 2025 Jan 30;15(1):3820. doi: 10

Type

Empty backbone

Expression

Bacterial

Promoter

pBAD

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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AA498

Plasmid

#231119

Purpose

Fragmid fragment: (guide cassette) ABE activity-based selection CD274 positive control

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

sgCD274 + ABE splice-targeting sgRNA

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA505

Plasmid

#231120

Purpose

Fragmid fragment: (guide cassette) CBE activity-based selection CD274 positive control

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

sgCD274 + CBE splice-targeting sgRNA

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMV47

Plasmid

#128866

Purpose

Position 1 module- IX301997 (yeast integrative region IX301997)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Yeast integrative region chrIX301997

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMET17p-CCG 316

Plasmid

#131166

Purpose

For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

Type

Empty backbone

Tags

CCG

Expression

Yeast

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMV46

Plasmid

#128865

Purpose

Position 1 module- VI260998 (yeast integrative region VI260998)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Yeast integrative region chrVI260998

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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