We narrowed to 3,800 results for: golden gate

1. pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

   Plasmid

   #182348

   Purpose

   mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXTK066 [Con-Pro_nptII-Broad_PartType8]

   Plasmid

   #229303

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_nptII-Broad_PartType8

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   nptII Promoter (Broad)
   

   Expression

   Bacterial

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pL0-BvDODAα1

   Plasmid

   #162528

   Purpose

   Beta vulgaris 4,5-DOPA dioxygenase alpha 1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Insert

   DODAα1
   

   Use

   Puc19-derived

   Expression

   Bacterial

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-T3G-CERP2

   Plasmid

   #175498

   Purpose

   All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)

   Plasmid

   #178988

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX459-sgRNA048_Grb10

   Plasmid

   #232934

   Purpose

   Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   Cas9-T2A-PuroR (Grb10 S. pyogenes)
   

   Expression

   Mammalian

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNP561

   Plasmid

   #222723

   Purpose

   Target and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Type

   Empty backbone

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-CT3G-CERP2

   Plasmid

   #175499

   Purpose

   All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. EC71100

   Plasmid

   #154068

   Purpose

   Level 0 Golden Gate vector, pHvHSP17

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   pHvHSP17
   

   Use

   Standard cloning vector used by geneart for synth…

   Mutation

   All BsaI, Esp3I and BpiI restriction sites were r…

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTE5400_pMBP_CDS dropout vector

    Plasmid

    #221189

    Purpose

    Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Type

    Empty backbone

    Tags

    10x His-tag + E. coli maltose binding protein (MB…

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZmUbi-5'UTR

    Plasmid

    #154057

    Purpose

    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    Promoter and 5' UTR Ubiquitin (Zea mays)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    N/A

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC71022

    Plasmid

    #159608

    Purpose

    Level 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxP

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    loxP-mCherry-HDEL-t35S-loxP
    

    Use

    Cre/Lox and Synthetic Biology

    Mutation

    All BsaI, Esp3I and BPiI restriction sites were r…

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pL0-MtPT4Pro

    Plasmid

    #159414

    Purpose

    Medicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    MtPT4 Promoter
    

    Use

    Puc19-derived

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pL0-MtBCP1Pro

    Plasmid

    #159415

    Purpose

    Medicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    MtBCP1 Promoter
    

    Use

    Puc19-derived

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-T3G-ERP2

    Plasmid

    #175188

    Purpose

    All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pShoHELIX-sgRNA_entry (BO3)

    Plasmid

    #181784

    Purpose

    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBT29-splitD

    Plasmid

    #122599

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT29-splitC

    Plasmid

    #122598

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCas9-SunTag(10X)-2A-BFP

    Plasmid

    #227389

    Purpose

    Adhesion module, dCas9-SunTag

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    dCas9-SunTag
    

    Use

    Lentiviral

    Mutation

    Enzymatically dead Cas9

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNP555

    Plasmid

    #222722

    Purpose

    sfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Type

    Empty backbone

    Tags

    sfGFP

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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