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pEBB COMMD3 Flag

Plasmid

#74895

Purpose

Express COMMD3 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD3 (COMMD3 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pGM1190

Plasmid

#69994

Purpose

OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Expression

Bacterial

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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HA-Halo-HSF2

Plasmid

#200202

Purpose

Expression of full length HSF2

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HA-Halo-HSF2 (Hsf2 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hCaspase-8

Plasmid

#208786

Purpose

Inducibly expresses human Caspase 8 fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Caspase 8 (CASP8 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

CARD domain is removed

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-145

Plasmid

#118529

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSC101-Donor

Plasmid

#140630

Purpose

Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

LE, RE, KanR, temperature sensitive pSC101 origin of replication

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-153

Plasmid

#118567

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-157

Plasmid

#118571

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVTRE3_tdTomato

Plasmid

#135178

Purpose

AAV expression of TRE(TRE3G)-driven, tdTomato expression for fluorescent labling

Depositor

Tetsuo Yamamori

Article

Abe et al Front Neuroanat. 2018 Oct 30;12:89. doi:

Inserts

tdTomato
TRE3G

Use

AAV

Expression

Mammalian

Mutation

a variant of DsRed

Promoter

TRE3G

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-mRuby3

Plasmid

#166136

Purpose

Gateway entry vector for mRuby3

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

mRuby3

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLdCN

Plasmid

#84290

Purpose

Express gRNA and Cas9 in Leishmania with Neomycin resistance

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Promoter

L. donovani ribosome RNA promoter

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Myc-IRES-RFP

Plasmid

#35395

Depositor

Martine Roussel

Article

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Myc (Myc Mouse)
RFP

Use

Retroviral

Expression

Mammalian

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
pMJ922

Plasmid

#78312

Purpose

Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

Depositor

Martin Jinek

Article

Burger et al Development. 2016 Apr 29. pii: dev.13480

Insert

SpCas9

Tags

HA-2xNLS-EGFP-NLS and His6-MBP-TEV

Expression

Bacterial

Mutation

human codon-optimized gene sequence

Promoter

T7

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLIX401 APE1 WT

Plasmid

#227190

Purpose

Inducible lentiviral expression of WT APE1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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GRIK1_pCSdest

Plasmid

#53832

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

GRIK1 (GRIK1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA5 FRT TO myc hSeparase

Plasmid

#59820

Purpose

Allows the integration of myc hSeparase in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-ccdB

Plasmid

#166145

Purpose

Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

His10

Expression

Bacterial

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pmRE-Tn5-147

Plasmid

#118526

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-mMLKL

Plasmid

#208784

Purpose

Inducibly expresses mouse MLKL fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

mCherry mMLKL Cry2 (Mlkl Mouse)

Use

Lentiviral

Tags

Cry2 and mCherry

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-146

Plasmid

#118530

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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