3,636 results

1. pMCh-76

   Plasmid

   #92288

   Purpose

   Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cells

   Depositor

   Marina Chekulaeva, Roy Parker

   Article

   Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

   Insert

   dGW182 CED (gw Fly)
   

   Tags

   NHA

   Expression

   Insect

   Mutation

   dGW182 CED: aa 940-1215

   Promoter

   Ac5

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMCh-646

   Plasmid

   #92289

   Purpose

   Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cells

   Depositor

   Marina Chekulaeva, Witold Filipowicz

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   dGW182 CED 7W7A (gw Fly)
   

   Tags

   NHA

   Expression

   Insect

   Mutation

   dGW182 CED: aa 940-1215 with the following point …

   Promoter

   Ac5

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCh-59

   Plasmid

   #92261

   Purpose

   Plasmid for expression of HA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cells

   Depositor

   Marina Chekulaeva, Roy Parker

   Article

   Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

   Insert

   dGW182 (gw Fly)
   

   Tags

   HA

   Expression

   Insect

   Mutation

   aa 940-1215

   Promoter

   Ac5

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSTAP-seq_fly-zfh1_spikeIn-1

   Plasmid

   #86387

   Purpose

   Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter
   

   Use

   Stap-seq spike in vector

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSTAP-seq_fly-zfh1_spikeIn-3

   Plasmid

   #86389

   Purpose

   Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#1
   

   Use

   Stap-seq spike in vector

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSTAP-seq_fly-zfh1_spikeIn-4

   Plasmid

   #86390

   Purpose

   Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#2
   

   Use

   Stap-seq spike in vector

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3_zfh1_CP-candidate_luc+

   Plasmid

   #86391

   Purpose

   Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer
   

   Use

   Luciferase

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pSTAP-seq_fly-ssp3

   Plasmid

   #86382

   Purpose

   Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster ssp3 enhancer
   

   Use

   Stap-seq screening vector

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSTAP-seq_fly-ham

   Plasmid

   #86383

   Purpose

   Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster ham enhancer
   

   Use

   Stap-seq screening vector

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSTAP-seq_fly-sgl

    Plasmid

    #86384

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster sgl enhancer
    

    Use

    Stap-seq screening vector

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSTAP-seq_fly-ncm

    Plasmid

    #86385

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster ncm enhancer
    

    Use

    Stap-seq screening vector

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSTAP-seq_fly-zfh1_spikeIn-2

    Plasmid

    #86388

    Purpose

    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer & D. pseudoobscura CG32369 core promoter
    

    Use

    Stap-seq spike in vector

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc-GFP-dSUMOdeltaC

    Plasmid

    #85697

    Purpose

    expression of GFP-SUMO1 delta C in Drosophila cells

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Tags

    GFP

    Expression

    Insect

    Mutation

    non-conjugatable SUMO, lacking the C-terminal gly…

    Promoter

    pAc5

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

14. exon K-renilla

    Plasmid

    #81007

    Purpose

    luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

    Depositor

    Richard Baines

    Article

    Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

    Insert

    paralytic (para Fly)
    

    Tags

    Renilla luciferase

    Expression

    Insect

    Mutation

    spanning exon 24 to exon 26, termination codon in…

    Promoter

    P-AC5 (Actin 5C)

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. UAS-Xbp1spliced

    Plasmid

    #80628

    Purpose

    expression of Xbp1 RB isoform in Drosophila

    Depositor

    Pedro Domingos, Herman Steller

    Article

    Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2

    Insert

    Xbp1 (Xbp1 Fly)
    

    Expression

    Insect

    Mutation

    RB isoform

    Promoter

    hsp70

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pDHJS3 AN+

    Plasmid

    #78240

    Purpose

    One of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).

    Depositor

    Tao-shih Hsieh

    Article

    Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

    Inserts

    loxP
    sequence "A" with NheI site
    loxP2
    

    Use

    Phagemid

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDHJS3 BS-

    Plasmid

    #78239

    Purpose

    One of four plasmids needed to create the double Holliday junction substrate (DHJS).

    Depositor

    Tao-shih Hsieh

    Article

    Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

    Inserts

    loxP
    sequence "A"
    loxP2
    

    Use

    Phagemid

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDHJS3 AS+

    Plasmid

    #78236

    Purpose

    One of four plasmids needed to create the double Holliday junction substrate (DHJS).

    Depositor

    Tao-shih Hsieh

    Article

    Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

    Inserts

    loxP
    sequence "A"
    loxP2
    

    Use

    Phagemid

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-FLAG-dSestrin (CG11299-PD)

    Plasmid

    #73675

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn (variant D) (Sesn Fly)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pFOSGE_CUS1E07

    Plasmid

    #71086

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Taf13 (Taf13 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only