We narrowed to 22,746 results for: Sele

1. TA1-DuET

   Plasmid

   #213382

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   TA1 (TAAR1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

   Plasmid

   #102887

   Purpose

   PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

   Plasmid

   #102888

   Purpose

   PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FPR3-DuET

   Plasmid

   #213241

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   FPR3 (FPR3 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hNR2F2-RFP

   Plasmid

   #22926

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   human nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress (NR2F2 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. aMHC-Puro Rex-Blast

   Plasmid

   #21231

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   alpha myosin heavy chain promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA_B2AR-i-pep

   Plasmid

   #47436

   Purpose

   FRET sensor for beta2-adrenergic receptor

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

   Insert

   beta2-adrenergic receptor (ADRB2 Human)
   

   Use

   Tags

   10 nm ER/K helix, HA, i-pep, mCerulean, and mCitr…

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pQStrep2-ARPC3

   Plasmid

   #31580

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   Actin related protein 2/3 complex, subunit 3 (ARPC3 Human)
   

   Use

   Tags

   His and StrepII-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBacI-KDAC8 D101E

   Plasmid

   #224350

   Purpose

   Expresses human KDAC8 (HDAC8) D101E in insect cells

   Depositor

   Terry Watt

   Article

   Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

   Insert

   KDAC8 (HDAC8 Human)
   

   Use

   Tags

   TEV-cleavable His6

   Expression

   Insect

   Mutation

   Aspartate 101 mutated to glutamate

   Promoter

   Polyhedrin

   Available since

   Oct. 22, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. AID-mTagBFP2-loxP_myo2_neoR-loxP

    Plasmid

    #194055

    Purpose

    Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    codon optimized mTagBFP2

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221_SLC39A8

    Plasmid

    #132177

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A8 (SLC39A8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pblic-neo TRX1

    Plasmid

    #110919

    Purpose

    To overexpress Thioredoxin-1

    Depositor

    Priyamvada Rai

    Article

    Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

    Insert

    TRX1 (TXN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mTiam1-mcherry-sspB in pcDNA3.1

    Plasmid

    #85221

    Purpose

    Role of Rac selective GEF domain from Tiam1 in immune cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Inserts

    mTiam1 (1030-1406) (Tiam1 Mouse)
    mcherry
    sspB
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEBTet-SNAP-Centrobin

    Plasmid

    #136829

    Purpose

    Mammalian expression of the centrosomal protein Centrombin N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Centrobin (CNTROB Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Agarabi CHO Light Chain in pJ201

    Plasmid

    #111566

    Purpose

    Cloning vector containing CDS for the murine light chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

    Depositor

    Cyrus Agarabi

    Article

    Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

    Insert

    Murine light chain
    

    Use

    Synthetic Biology; Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. ATP1A1_T804N_hPGK1_EBFP_Donor

    Plasmid

    #187455

    Purpose

    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1-T804N EBFP donor (ATP1A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK1

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQTEV-MAP1LC3A

    Plasmid

    #34824

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEN25-Ub Hs

    Plasmid

    #83232

    Purpose

    pHis-par2-Ub Hs

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    Ubiqutin (UBC Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA_B2AR-no-pep

    Plasmid

    #47439

    Purpose

    control FRET sensor for beta2-adrenergic receptor

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

    Insert

    beta2-adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    10 nm ER/K helix, HA, mCerulean, mCitrine, and no…

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pACE-K2P10.1-StrepTagII

    Plasmid

    #191475

    Purpose

    Expresses K2P10.1 with C-terminal StrepTagII

    Depositor

    Arthur Laganowsky

    Article

    Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

    Insert

    K2P10.1 (KCNK10, TREK-2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    N144Q (uniprot P57789-1)

    Promoter

    polH

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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