We narrowed to 13,284 results for: sequence
-
Plasmid#217635PurposeAAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.DepositorInsertmU6-sgRNA, Handle sequence, EF1a-mTagBFP2
UseAAVPromoterEF1alpha and mU6Available SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
EK0438 SFFV-mCherry-SGc(s70587)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191163PurposeExpression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).DepositorInsertmCherry:s70587:EGFP-3xMS2
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRL838
Plasmid#70680PurposeThis vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.DepositorTypeEmpty backboneUseCre/LoxAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL003
Plasmid#215335PurposeamyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)DepositorTypeEmpty backboneExpressionBacterialPromoterpSpankAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOpen-DNA Topoisomerase 1B Vaccinia Virus
Plasmid#165510PurposeUsed in TOPO cloning. Recognizes the DNA sequence 5'-(C/T)CCTT-3' and digests double stranded DNA at this sequence.DepositorInsertDNA Topoisomerase 1B Vaccinia Virus
UseSynthetic BiologyAvailable SinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG497 mito-iFAST
Plasmid#130819PurposeExpresses mito-iFAST in mammalian cells (mitochondria labeling)DepositorInsertmito-iFAST
Tagsmyc-tagExpressionMammalianPromoterCMVAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKIIa-C1V1::FusionRed::Kv2.1
Plasmid#102771Purpose"Activate neurons with 2p stimulation"DepositorInsertC1V1::FusionRed::Kv2.1
UseAAVTagsFusionRed and Kv2.1 traficking domainAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9E
Plasmid#228419PurposeExpression of NIT-9E domain in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaMutationtruncation of the N terminal G domain and linkageā¦Available SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9G
Plasmid#228420PurposeExpression of NIT-9G domain in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaMutationtruncation of the C terminal E domain and linkageā¦Available SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9_A184-G203
Plasmid#228421PurposeExpression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaMutationtruncation of A184-G203Available SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only