We narrowed to 3,800 results for: golden gate

1. pKG2197_pPV2-ZF10-NS3-p65

   Plasmid

   #239700

   Purpose

   BsaI Golden Gate assembly plasmid encoding the SynZiFTR ZF10-NS3-p65 CDS

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Insert

   ZF10-NS3-p65
   

   Use

   Synthetic Biology

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. SMT503

   Plasmid

   #226840

   Purpose

   Plasmid that encodes a B. Subtilis lipase (BsLipA) gene fusion with N-terminal SNAP and C-Terminal eGFP under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. Chloramp

   Depositor

   Polly Fordyce

   Article

   Thompson et al Adv Mater Interfaces 2024, 12 (3), 24004

   Insert

   Lipase A
   

   Use

   Synthetic Biology

   Tags

   SNAP and eGFP

   Expression

   Bacterial

   Mutation

   R33Q, D34N, K35D, K112D, M134D, Y139C, I157M

   Promoter

   T7

   Available Since

   Aug. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNS1D-sgRNA9-SpR

   Plasmid

   #191092

   Purpose

   aTc inducible gRNA expression plasmid with BbsI sites for Golden Gate insertion of crRNA oligos and homology arms for genome integration at NS1D in Synechococcus sp. PCC 7002

   Depositor

   Anne Ruffing

   Article

   Dallo et al ACS Synth Biol. 2023 Apr 21;12(4):1175-1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   tet

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEVAb827

   Plasmid

   #217500

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb82 backbone, RK2 ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEVAb837

   Plasmid

   #217501

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSEVAb847

   Plasmid

   #217502

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb84 backbone, pRO1600/ColE1 ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSEVAb867

   Plasmid

   #217503

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb86 backbone, p15A ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSEVAb887

   Plasmid

   #217504

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb88 backbone, pUC ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSEVAb897

   Plasmid

   #217505

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAD183_gCAD_pET

    Plasmid

    #239119

    Purpose

    Gorilla ACOD1 in E.coli expression vector

    Depositor

    Konrad Buessow

    Article

    Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

    Insert

    ACOD1
    

    Tags

    StrepTag-II and TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKG3668_pGEEC578_pShip-EFS-rtTA-P2A-iRFP720-bGH

    Plasmid

    #239741

    Purpose

    Plasmid expressing a doxycycline-inducible transcriptional activator

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    rtTA-P2A-iRFP720
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKG2499_pGEEC531_pShip-CMV-mRuby2-WPRE

    Plasmid

    #239727

    Purpose

    Plasmid expressing mRuby2 with the CMV promoter and WPRE 3' UTR

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKG2906_pGEEC546_pShip-EFS-tagBFP-bGH

    Plasmid

    #239734

    Purpose

    Plasmid expressing tagBFP with the EFS promoter

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    tagBFP
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKG2907_pGEEC547_pShip-hPGK-tagBFP-bGH

    Plasmid

    #239735

    Purpose

    Plasmid expressing tagBFP with the hPGK promoter

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    tagBFP
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKG2897_pGEEC548_pShip-UbC-tagBFP-bGH

    Plasmid

    #239736

    Purpose

    Plasmid expressing tagBFP with the UbC promoter

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    tagBFP
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKG2899_pGEEC550_pShip-CMV-tagBFP-bGH

    Plasmid

    #239737

    Purpose

    Plasmid expressing tagBFP with the CMV promoter

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    tagBFP
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKG2900_pGEEC551_pShip-CAG-tagBFP-bGH

    Plasmid

    #239738

    Purpose

    Plasmid expressing tagBFP with the CAG promoter

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    tagBFP
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKG2023_pGEEC509_pShip-UbC-mRuby2-WPRE

    Plasmid

    #239715

    Purpose

    Plasmid expressing mRuby2 with the UbC promoter and WPRE 3' UTR

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKG2116_pGEEC510_pShip-EF1a-mRuby2-bGH

    Plasmid

    #239716

    Purpose

    Plasmid expressing mRuby2 with the EF1a promoter and bGH 3' UTR

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKG2117_pGEEC511_pShip-EF1a-mRuby2-SV40

    Plasmid

    #239717

    Purpose

    Plasmid expressing mRuby2 with the EF1a promoter and SV40 3' UTR

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge