We narrowed to 1,429 results for: MiCy

1. pFBD-BirA_LRRK2:2141-2527

   Plasmid

   #210901

   Purpose

   Insect expression of human LRRK2 fragment T2141 to E2527. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   LRRK2:T2141-E2527 (LRRK2 Human)
   

   Use

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Expression

   Insect

   Mutation

   M2397T natural variant ( VAR_024965)

   Promoter

   Polyhedrin

   Available since

   March 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pBacMam2-DiEx-LIC_LRRK2:2141-2527

   Plasmid

   #210900

   Purpose

   Insect expression of human LRRK2 fragment T2141 to E2527. C-terminal 10X His and Flag tag with TEV cleavage site.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   LRRK2:T2141-E2527 (LRRK2 Human)
   

   Use

   Tags

   AENLYFQSHHHHHHHHHHDYKDDDDK and M

   Expression

   Insect

   Mutation

   M2397T natural variant ( VAR_024965)

   Promoter

   P10 and CMV

   Available since

   March 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pFBD-BirA_CDC20:161-P477

   Plasmid

   #210888

   Purpose

   Insect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   CDC20:S161-P477 (CDC20 Human)
   

   Use

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Expression

   Insect

   Mutation

   wild type

   Promoter

   Polyhedrin

   Available since

   March 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pFBD-BirA_CDC40:236-578

   Plasmid

   #210885

   Purpose

   Insect expression of human CDC40 fragment E236 to W578. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   CDC40:E236-W578 (CDC40 Human)
   

   Use

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Expression

   Insect

   Mutation

   wild type

   Promoter

   Polyhedrin

   Available since

   March 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pFBD-BirA_DCAF1:1038-1400

   Plasmid

   #210883

   Purpose

   Insect expression of human DCAF1 fragment E1038 to E1400. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   DCAF1:E1038-E1400 (DCAF1 Human)
   

   Use

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Expression

   Insect

   Mutation

   wild type

   Promoter

   Polyhedrin

   Available since

   March 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)

   Plasmid

   #169076

   Purpose

   Insect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepII

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Inserts

   ATG5 (ATG5 Human)
   ATG7 (ATG7 Human)
   ATG10 (ATG10 Human)
   ATG12 (ATG12 Human)
   ATG16 (ATG16L1 Human)
   

   Use

   Tags

   GGlinker, TEV cleavage site, StrepII and HIS10 Ta…

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Polyhedrin promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

   Plasmid

   #111903

   Purpose

   Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.

   Depositor

   Andrew Carter

   Inserts

   His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
   DYNC1I2 (DYNC1I2 Human)
   DYNC1LI2 (DYNC1LI2 Human)
   DYNLT1 (DYNLT1 Human)
   DYNLRB1 (DYNLRB1 Human)
   DYNLL1 (DYNLL1 Human)
   

   Use

   Tags

   8xHis, SNAPf-tag, TEV site, and ZZ-tag

   Expression

   Insect

   Mutation

   Optimised for expression in Sf9 cells.

   Promoter

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

   Plasmid

   #169077

   Purpose

   Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Inserts

   ATG5 (ATG5 Human)
   ATG7 (ATG7 Human)
   ATG10 (ATG10 Human)
   ATG12 (ATG12 Human)
   ATG16 (ATG16L1 Human)
   

   Use

   Tags

   GFP tag, GGlinker, TEV cleavage site. StrepII and…

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Polyhedrin promoter

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Dynein-2_complex

   Plasmid

   #132536

   Purpose

   Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Inserts

   DYNC2H1 (DYNC2H1 Human)
   DYNC2LI1 (DYNC2LI1 Human)
   WDR34 (WDR34 Human)
   WDR60 (WDR60 Human)
   DYNLL1 (DYNLL1 Human)
   DYNLL2 (DYNLL2 Human)
   DYNLRB1 (DYNLRB1 )
   DYNLRB2 (DYNLRB2 Human)
   DYNLT1 (DYNLT1 Human)
   TCTEX1D2 (TCTEX1D2 Human)
   DYNLT3 (DYNLT3 Human)
   LC8-like (LOC392067 Human)
   

   Use

   Tags

   8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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