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Plasmid#149580Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only
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pBSV2H_Psyn-sgRNAftsI
Plasmid#149563PurposeftsI gRNA expression in B. burgdorferiDepositorInsertsgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAmreB
Plasmid#149567PurposemreB gRNA expression in B. burgdorferiDepositorInsertsgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM54
Plasmid#154157PurposeFirefly luciferase under the control of 8 copies of the Aedes aegypti Rel2 NF-κB siteDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoter8 copies of the Aedes aegypti Rel2 NF-κB siteAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_023
Plasmid#123953PurposeEncodes human RelA as a Type 3b part to be used in the MTK systemDepositorInsertRelA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAS attB V5 dDispatched WT HA
Plasmid#126411PurposeExpression of wild type dDispatched HA in Drosophila.DepositorInsertd-Dispatched1 (disp Fly)
UseTagsHAExpressionInsectMutationPromoterAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAS-attB V5 dDispatched delta CS HA
Plasmid#126412PurposeExpression of V5 dDispatched Delta CS HA in Drosophila cells linesDepositorInsertd-Dispatched1 (disp Fly)
UseTagsHAExpressionInsectMutationDisp1 Cleavage sitePromoterAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-ATG1(mut)(406)
Plasmid#122069PurposeExpressing a mutant ATG1DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationCodon changes without changing the amino acid seq…PromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-ATG7p-ATG1(406)
Plasmid#122071PurposeExpressing ATG1DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationThe native promoter was replaced by ATG7 promoter…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(D195A,E196A)(405)
Plasmid#122065PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
UseTagsPAExpressionYeastMutationAspartic acid 195 to alanine; Glutamic acid 196 t…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(S226A,T228A)(405)
Plasmid#122066PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
UseTagsPAExpressionYeastMutationSerine 226 to alanine; Threonine 228 to alaninePromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mRuby-N
Plasmid#113752PurposeGateway multi-site entry clone for second position mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2
UseCRISPR; Gateway entry vectorTagsmRuby2ExpressionMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmEGFP-N
Plasmid#113747PurposeGateway multi-site entry clone for second position 2x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags2xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmEGFP-N
Plasmid#113748PurposeGateway multi-site entry clone for second position 3x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmEGFP-C
Plasmid#113750PurposeGateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags2xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-N
Plasmid#113753PurposeGateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-C
Plasmid#113756PurposeGateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-R1ACR_6367-EYFP
Plasmid#111080PurposeAnion channelrhodopsin from Rhodomonas sp. for expression in mammalian cellsDepositorInsertAnion channelrhodopsin R1ACR_6367
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RlACR_497-EYFP
Plasmid#111078PurposeAnion channelrhodopsin from Rhodomonas lens for expression in mammalian cellsDepositorInsertAnion channelrhodopsin RlACR_497
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry
Plasmid#68276PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLSDepositorInsertSte7DS NLS(4E) mCherry
UseTagsmCherryExpressionYeastMutationPromoterRPS2Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only