We narrowed to 7,284 results for: Tet-on

1. p3015b

   Plasmid

   #209317

   Purpose

   Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

   Depositor

   Thomas Hooven

   Article

   Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

   Insert

   Group B Streptococcus-compatible single guide RNA
   

   Expression

   Bacterial

   Promoter

   Xyl/tet promoter that is constitutively active

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICE-NLS-mCherry

   Plasmid

   #60364

   Purpose

   Plasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Insert

   NLS-mCherry
   

   Tags

   NLS from SV40 T antigen and mCherry

   Promoter

   CMV-tet

   Available Since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

   Plasmid

   #216253

   Purpose

   FRET-based calcium sensor located in the ER lumen, Tet-inducible

   Depositor

   Edward Avezov

   Article

   Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

   Insert

   ER-targeted D4 second generation cameleon (FRET calcium sensor)
   

   Use

   Lentiviral

   Tags

   REAch and mTurquoise

   Promoter

   TRE3G

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TRE-DIO-mNeonGreen

   Plasmid

   #99132

   Purpose

   An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mNeonGreen

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   DIO-mNeonGreen
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRevTRE-hygro-GFP-Crumbs3a WT

   Plasmid

   #195217

   Purpose

   Retroviral Tet response vector containing GFP tagged Crumbs3a

   Depositor

   Ben Margolis, David Bryant

   Article

   Schluter et al Mol Biol Cell. 2009 Nov;20(22):4652-63.

   Insert

   CRB3A (CRB3 Human)
   

   Use

   Retroviral

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TRE-DIO-mTurquoise2

   Plasmid

   #99115

   Purpose

   An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mTurquoise2

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   DIO-mTurquoise2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. 908H

   Plasmid

   #160240

   Purpose

   TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   Tet Operator (2x)
   hsp70 minimal promoter
   p-element promoter
   Firefly luciferase
   Renilla luciferase
   Ubiquitin promoter
   

   Tags

   T2A GFP

   Expression

   Insect

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe-Ypet-PBD

   Plasmid

   #24533

   Depositor

   Klaus Hahn

   Article

   Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

   Insert

   PAK binding domain (PAK1 Human)
   

   Use

   Retroviral

   Tags

   Ypet

   Expression

   Mammalian

   Available Since

   Aug. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5 FRT TO GFP hBub1 K821R

   Plasmid

   #59813

   Purpose

   Allows the integration of GFP hBub1 K821R in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

   Insert

   Bub1 (BUB1 Human)
   

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   K821R

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. MTK3_010

    Plasmid

    #123725

    Purpose

    Encodes Tet-On 3G transactivator protein as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    TetOn3G
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe-CyPet-Rac1

    Plasmid

    #24534

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Retroviral

    Tags

    CyPet

    Expression

    Mammalian

    Available Since

    Aug. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-TRE-DIO-mRuby2

    Plasmid

    #99117

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    DIO-mRuby2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5 FRT TO GFP hBub1 WT

    Plasmid

    #59812

    Purpose

    Allows the integration of GFP hBub1 in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

    Insert

    Bub1 (BUB1 Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. RT3GEN

    Plasmid

    #111165

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Mutation

    WT

    Promoter

    T3G

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p6898 PHAGE-P CMVT N-HA-hE6AP I C820A

    Plasmid

    #37602

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP I C820A (UBE3A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C820A

    Promoter

    CMV tet regulatable

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

16. RT3GEP

    Plasmid

    #111164

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Mutation

    WT

    Promoter

    T3G

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. RT3REN

    Plasmid

    #111166

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi with DsRed reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Mutation

    WT

    Promoter

    T3G

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-DIO-eYFP-f

    Plasmid

    #104057

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequence

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP-f
    

    Use

    AAV

    Promoter

    TRE

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRE-eGFP-F1C

    Plasmid

    #211457

    Purpose

    Dox inducible delivery of eGFP fused to a flotillin-1 palmitoylation blocking peptide (F1C)

    Depositor

    Linda DeGraffenried

    Article

    McClellan et al Oncogene. 2024 Mar;43(14):1063-1074. doi

    Insert

    Flotillin-1-palmitoylation blocking peptide (FLOT1 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRE-eGFP-F1A

    Plasmid

    #211458

    Purpose

    Dox inducible delivery of eGFP fused to a flotillin-1 palmitoylation mutant control peptide (F1A)

    Depositor

    Linda DeGraffenried

    Article

    McClellan et al Oncogene. 2024 Mar;43(14):1063-1074. doi

    Insert

    Flotillin-1-palmitoylation mutant control peptide (FLOT1 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    C34A

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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