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Showing: 1431 - 1440 of 13280 results

  3. pMVRha

    Plasmid
    #229553
    Purpose
    Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.
    Depositor
    Sandy Schmidt
    Article
    Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    229553-plasmid-map-sequence-id-461178

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  4. pABE-dGFP

    Plasmid
    #226863
    Purpose
    Constitutively expresses mCherry as a transfection marker. Expresses non-fluorescent EGFP, deactivated by A111V mutation, that can be reactivated by active adenine base editing
    Depositor
    Alexis Komor
    Article
    Osgood et al Methods in Enzymology, February 5, 2025
    Insert
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    A111V
    Promoter
    CMV
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226863-plasmid-map-sequence-id-450497

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  5. pCBE-dGFP

    Plasmid
    #226861
    Purpose
    Constitutively expresses mCherry as a transfection marker. Expresses non-fluorescent EGFP, deactivated by Y93H mutation, that can be reactivated by active cytosine base editing
    Depositor
    Alexis Komor
    Article
    Osgood et al Methods in Enzymology, February 5, 2025
    Insert
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y93H
    Promoter
    CMV
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226861-plasmid-map-sequence-id-450494

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  6. pLN34 - pBait-5GC[1xMS2]-McaS

    Plasmid
    #222403
    Purpose
    arabinose-induced synthesis of a hybrid RNA with an MS2 hairpin surrounded by a 5bp GC clamp with E coli McaS inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.
    Depositor
    Katherine Berry
    Article
    Linh D. Nguyen et al Sci Rep 15, 3820 (2025)
    Insert
    McaS (mcaS E. coli)
    Use
    Tags
    1xMS2 hairpin, 5GC clamp
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    222403-plasmid-map-sequence-id-451849

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  7. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

    Plasmid
    #99696
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV
    Depositor
    George Church
    Insert
    dCas9 and gRNA targeting Afp (Afp Synthetic)
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    99696-plasmid-map-sequence-id-194593

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  8. SUMO_MT3

    Plasmid
    #200303
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    metallothionein 3 (MT3 Human)
    Use
    Tags
    Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200303-plasmid-map-sequence-id-394864

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  9. CD44I-c014

    Plasmid
    #175451
    Purpose
    Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Use
    Tags
    AviTag-His6
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175451-plasmid-map-sequence-id-353223

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  10. CD44U-c011

    Plasmid
    #175454
    Purpose
    Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Use
    Tags
    AviTag-His6
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175454-plasmid-map-sequence-id-353220

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Showing: 1431 - 1440 of 13280 results