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pDONR221-LETMD1_STOP

Plasmid

#161460

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

LETMD1 (LETMD1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-PIK3CA-P539R

Plasmid

#116525

Purpose

Lentiviral expression of PIK3CA P539R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

P539R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-M1043I

Plasmid

#116510

Purpose

Lentiviral expression of PIK3CA M1043I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

M1043I

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-M1043V

Plasmid

#116512

Purpose

Lentiviral expression of PIK3CA M1043V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

M1043V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-N345K

Plasmid

#116517

Purpose

Lentiviral expression of PIK3CA N345K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

N345K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-H1047Y

Plasmid

#116501

Purpose

Lentiviral expression of PIK3CA H1047Y

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

H1047Y

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-K111delK

Plasmid

#116505

Purpose

Lentiviral expression of PIK3CA K111delK

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

K111delK

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CA_p.E726K

Plasmid

#82845

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PIK3CA (PIK3CA Human)

Use

Gateway Cloning

Mutation

E726K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Cherry-SRBC

Plasmid

#69492

Purpose

Expression of fluorescently tagged SRBC in mammalian cells

Depositor

Rob Parton

Article

Gambin et al Elife. 2013 Jan 1;3:e01434. doi: 10.7554

Insert

SRBC (Cavin3 Mouse)

Tags

Cherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only
mPapaya1-Actin-C-18

Plasmid

#56400

Purpose

Localization: Actin, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Actin (ACTB Human)

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pET41a-MED19

Plasmid

#15430

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Tags

GST and His

Expression

Bacterial

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pET41a-MED30

Plasmid

#15431

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED30 (Med30 Mouse)

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pUb FLAG-tws

Plasmid

#195068

Purpose

Expresses FLAG-tagged Drosophila tws protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

twins (tws Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Sema3c(S)-AP-His

Plasmid

#72016

Purpose

Expresses the Sema3C protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Actin-C-18

Plasmid

#55400

Purpose

Localization: Actin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Actin (ACTB Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
DPP7 (3N0T)

Plasmid

#28114

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

DPP7:SDC134-D09:C41107 (DPP7 Human)

Tags

His

Expression

Bacterial

Mutation

contains amino acid residues 28-492

Available Since

March 18, 2011

Availability

Industry, Academic Institutions, and Nonprofits
pGEX-KG-MmUbc7/2783

Plasmid

#11490

Depositor

Allan Weissman

Article

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Insert

MmUbc7 (Ube2g2 Mouse)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pFastBac Mel18 flag

Plasmid

#1969

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Mel18 (Pcgf2 Mouse)

Tags

flag

Expression

Insect

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pT7-MLH1dn (mouse) for IVT

Plasmid

#246485

Purpose

Template for in vitro transcription of mouse MLH1dn

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

mouse MLH1dn (Mlh1 Mouse)

Use

Template for in vitro transcription

Mutation

deletion of residues 758-760

Promoter

T7

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_CUX1_NUTM1

Plasmid

#205800

Purpose

Express mEGFP-tagged fusion protein, CUX1_NUTM1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CUX1_NUTM1 (CUX1, NUTM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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