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pCS2+8-CYP2A6(C439A)-flag-tev-his

Plasmid

#227167

Purpose

For overexpression of CYP2A6 C439A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1(C295A)-flag-tev-halo-his

Plasmid

#227156

Purpose

For T-REX experiments of cyp-33e1 C295A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 295 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1(C439A)-flag-tev-halo-his

Plasmid

#227157

Purpose

For T-REX experiments of cyp-33e1 C439A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGB1-tail-mTurquoise2

Plasmid

#145142

Purpose

Shuttle vector with mTurquoise2-tagged ITGB1 cytoplasmic tail as an insert

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGB1-tail-mTurquoise2 (ITGB1 Human)

Use

Entry vector for gateway cloning

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGA2-tail-YPet

Plasmid

#145143

Purpose

Shuttle vector with YPet-tagged ITGA2 wild-type cytoplasmic tail as an insert

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGA2-tail-YPet (ITGA2 Human)

Use

Entry vector for gateway cloning

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGA2-tail-YPet (GFFKR/GAAKR)

Plasmid

#145144

Purpose

Shuttle vector with YPet-tagged ITGA2 cytoplasmic tail as an insert with a GFFKR/GAAKR mutation

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGA2-tail-YPet (GFFKR/GAAKR) (ITGA2 Human)

Use

Entry vector for gateway cloning

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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NLS-Cherry-PCNA-P2A-Venus-E2F3

Plasmid

#236448

Purpose

insect expression of E2F3 for protein purification

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F3 (E2f3 Mouse)

Expression

Insect

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pSMPPv2-Tau-RING (M72E/I18R)

Plasmid

#233690

Purpose

Expression of Tau-RING I18R-M72E mutant in mammalian cells

Depositor

Leo James

Article

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

Tau protein fused to TRIM21 RING domain carrying M72E/I18R mutations

Use

Lentiviral

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pOPTG-Tau-RING (M72E/I18R)

Plasmid

#233692

Purpose

Expression of Tau-RING I18R-M72E mutant in bacterial cells

Depositor

Leo James

Article

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

Tau protein fused to TRIM21 RING domain

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMV-TC1-2_DM

Plasmid

#217081

Purpose

Donor plasmid of TC1-2_DM Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of TC1-2_DM transposon

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMV-PROTOP

Plasmid

#217091

Purpose

Donor plasmid of PROTOP Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of PROTOP transposon

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMV-Tc1-8_Xt

Plasmid

#217092

Purpose

Donor plasmid of Tc1-8_Xt Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tc1-8_Xt transposon

Expression

Mammalian

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-TC1_FR2

Plasmid

#217085

Purpose

Donor plasmid of TC1_FR2 Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of TC1_FR2 transposon

Expression

Mammalian

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-MARISP1

Plasmid

#217049

Purpose

Donor plasmid of MARISP1 Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of MARISP1 transposon

Expression

Mammalian

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCJ1023

Plasmid

#228763

Purpose

Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

Depositor

Bradley Yoder

Insert

Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

human U6

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Mariner-2_XT

Plasmid

#217096

Purpose

Donor plasmid of Mariner-2_XT Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Mariner-2_XT transposon

Expression

Mammalian

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2386

Plasmid

#225639

Purpose

AAV plasmid with Ple388 (PITX3 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Fornes et al Nucleic Acids Res. 2023 Jul 5;51(W1):W37

Insert

Ple388 (PITX3 Human)

Use

AAV

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tc1-2_ST-10 kb

Plasmid

#218314

Purpose

Donor plasmid of Tc1-2_ST Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-MAG-10 kb

Plasmid

#218315

Purpose

Donor plasmid of MAG Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-8B_DR-10 kb

Plasmid

#218318

Purpose

Donor plasmid of Tc1-8B_DR Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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