We narrowed to 5,876 results for: nls

1. CRY-tTA (B702)

   Plasmid

   #92032

   Purpose

   Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   tTA2 (TetR fused to VP64)

   Expression

   Mammalian

   Mutation

   NLS on CRY2 is mutated

   Promoter

   CMV

   Available since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAN1735

   Plasmid

   #127204

   Purpose

   Dual inducible expression of SynTF-mScarlet-degronSwitchA and KeyA-BFP-NLS

   Depositor

   Hana El-Samad

   Article

   Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

   Insert

   pGal1-SynTF-mScarlet-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tENO1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Intersectin I SH3 D domain (human)

   Plasmid

   #47416

   Depositor

   Peter McPherson

   Article

   Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

   Insert

   Intersectin SH3 domain D (ITSN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   only SH3 domain D

   Promoter

   tac

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3 mouse Bmf

   Plasmid

   #17244

   Depositor

   Joan Brugge

   Article

   Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

   Insert

   Bmf (Bmf Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pCR1161

   Plasmid

   #111097

   Purpose

   Expresses FANCA_-_89883045.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCA gRNA (FANCA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCR1110

   Plasmid

   #111096

   Purpose

   Expresses BLM_+_91260645.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   BLM gRNA (BLM Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR223 PAK5 T538N

   Plasmid

   #60537

   Purpose

   Donor vector for human Pak5 T538N mutant

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   Pak5 (PAK5 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   T538N

   Promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pDONR223 PAK5 S573N

   Plasmid

   #60538

   Purpose

   Donor vector for human Pak5 S573N mutant

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   Pak5 (PAK5 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   S573N

   Promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCR1322

   Plasmid

   #111109

   Purpose

   Expresses FANCL_+_58468435.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCL gRNA (FANCL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR1271

    Plasmid

    #111106

    Purpose

    Expresses FANCI_-_89787239.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCI gRNA (FANCI Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p27.pA.NGFR.mCMV.hPGK.Rab35(S22N).wpre[sequenced]

    Plasmid

    #226766

    Purpose

    Rab35(S22N)

    Depositor

    Ferdinando Pucci

    Article

    Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

    Insert

    rab35-mutant S22N (RAB35 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p25.pA.NGFR.mCMV.hPGK.Rab35(WT).wpre[sequenced]

    Plasmid

    #226764

    Purpose

    rab35 (WT)

    Depositor

    Ferdinando Pucci

    Article

    Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

    Insert

    rab35 (RAB35 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p26.pA.NGFR.mCMV.hPGK.Rab35(Q67L).wpre[sequenced]

    Plasmid

    #226765

    Purpose

    Rab35(Q67L)

    Depositor

    Ferdinando Pucci

    Article

    Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

    Insert

    rab35-mutant Q67L (RAB35 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPB9v2

    Plasmid

    #210029

    Purpose

    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPB9

    Plasmid

    #210036

    Purpose

    Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    puro-T2A-eGFP
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SpCas9-NRCH-CGBE1

    Plasmid

    #198557

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-CGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3E-IRES-mTagBFP2-pA (JDW 1361)

    Plasmid

    #224535

    Purpose

    A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    IRES-FLAG-NLS-mTagBFP2-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHA850 - osm-10p::FynY531F

    Plasmid

    #139207

    Purpose

    Express activated Fyn kinase in osm-10 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Y531F

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHA852 - mec-4p::FynY531F

    Plasmid

    #139209

    Purpose

    Expresses activated Fyn kinase in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Y531F

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FUS_LC_withR

    Plasmid

    #139128

    Purpose

    express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    add arginines

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge