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Showing: 7301 - 7350 of 13635 results

  3. pGAPTrap-GFP

    Plasmid
    #82506
    Purpose
    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    82506-plasmid-map-sequence-id-156571

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  6. pRD399

    Plasmid
    #163106
    Purpose
    Expression of mEGFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_mEGFP_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163106-plasmid-map-sequence-id-321847

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  19. pDG461

    Plasmid
    #100902
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100902-plasmid-map-sequence-id-195323

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  22. CGBE1-NG (pBM1246)

    Plasmid
    #140256
    Purpose
    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP
    Depositor
    Keith Joung
    Article
    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103
    Insert
    eUNG-BE4max(R33A)_NG-ΔUGI
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140256-plasmid-map-sequence-id-291778

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  24. Cilantro 2

    Plasmid
    #74450
    Purpose
    Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Article
    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    74450-plasmid-map-sequence-id-140420

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  25. pDEST-CMV GFP-LC3B G120A

    Plasmid
    #123115
    Purpose
    Expresses EGFP-LC3B G120A in mammalian cells. Negative control fluorescent reporter, unable to localize to autophagosomes.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    CMV
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    123115-plasmid-map-sequence-id-244026

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  26. Cx32R220stop

    Plasmid
    #78151
    Purpose
    Mammalian Expression of the first 219 amino acids of Connexin 32 and EGFP from a bicistronic mRNA
    Depositor
    Mario Bortolozzi
    Article
    Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496
    Insert
    Connexin 32 (GJB1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Only contains first 219 residues
    Promoter
    CMV
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only

  30. pHRIG-AktDN

    Plasmid
    #53597
    Purpose
    lentiviral expression of dominant negative mouse Akt1 and EGFP
    Depositor
    Heng Zhao
    Article
    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12
    Insert
    Akt1 (Akt1 Mouse)
    Use
    Lentiviral
    Tags
    HA and IRES-EGFP
    Expression
    Mammalian
    Mutation
    Dominant negative (K179M)
    Promoter
    CMV
    Available since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only

  33. pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

    Plasmid
    #168106
    Purpose
    PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning
    Depositor
    Erich Jarvis
    Article
    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10
    Insert
    mCherry-KASH
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    168106-plasmid-map-sequence-id-330309

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  36. pHRIG-PRAS40

    Plasmid
    #53599
    Purpose
    lentiviral expression of constitutively active PRAS40 and EGFP
    Depositor
    Heng Zhao
    Article
    Xiong et al Neurobiol Dis. 2014 Jun;66:43-52. doi: 1
    Insert
    PRAS40 (AKT1S1 Human)
    Use
    Lentiviral
    Tags
    IRES-EGFP and myc
    Expression
    Mammalian
    Mutation
    constitutively active (myristoylated version)
    Promoter
    CMV
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. pssGFP-hCRT

    Plasmid
    #80022
    Purpose
    Mammalian expression of signal peptide-EGFP-humanCRT
    Depositor
    Chinten James Lim
    Article
    Liu et al Cell Death Dis. 2016 Jun 16;7:e2268. doi
    Insert
    CRT (CALR Human)
    Use
    Tags
    EGFP and signal peptide
    Expression
    Mammalian
    Mutation
    signal peptide 17aa added before GFP-CRT, will ta…
    Promoter
    CMV
    Available since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only

  47. PX3

    Plasmid
    #223339
    Purpose
    Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    ABCD1-miniTurboID-EGFP-3HA (PXA1 Mustard Weed)
    Use
    Tags
    miniTurboID-EGFP-3HA
    Expression
    Plant
    Mutation
    Promoter
    CaMV35S
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223339-plasmid-map-sequence-id-455820

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  48. PX1

    Plasmid
    #223337
    Purpose
    Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    ABCD1-TurboID-EGFP-3HA (PXA1 Mustard Weed)
    Use
    Tags
    TurboID-EGFP-3HA
    Expression
    Plant
    Mutation
    Promoter
    CaMV35S
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223337-plasmid-map-sequence-id-455823

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  49. MT4

    Plasmid
    #223332
    Purpose
    Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    3HA-miniTurboID-EGFP-OMP25 (Synj2bp Rat)
    Use
    Tags
    3HA-miniTurboID-EGFP
    Expression
    Plant
    Mutation
    Promoter
    CaMV35S
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    223332-plasmid-map-sequence-id-455589

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  50. pRD441

    Plasmid
    #168467
    Purpose
    For the insertion pf NLS-mEGFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS-mEGFP-H-NSdbd-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168467-plasmid-map-sequence-id-331917

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Showing: 7301 - 7350 of 13635 results