We narrowed to 5,180 results for: pcDNA3.1+

1. pEEF1D-FLAG (pcDNA 3.1+) LG6-1

   Plasmid

   #37365

   Depositor

   David Litchfield

   Article

   Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

   Insert

   EEF1D (EEF1D Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2012

   Availability

   Academic Institutions and Nonprofits only

2. ER-mGreenLantern

   Plasmid

   #164463

   Purpose

   Localization and retention of mGreenLantern fluorescent protein in the ER

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FLAG-MED18

   Plasmid

   #15419

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED18 (MED18 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. hHSF1 S303/7A

   Plasmid

   #118365

   Purpose

   This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   S303A and S307A

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Myc-RILPL1

   Plasmid

   #201470

   Purpose

   For overexpression in cultured cells to visualize RILPL1 protein interactions.

   Depositor

   Suzanne Pfeffer

   Article

   Pal et al Sci Adv. 2023 Dec 15;9(50):eadj1205. doi

   Insert

   RILPL1 (RILPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-sACE2(WT)-8his

   Plasmid

   #149268

   Purpose

   Mammalian expression plasmid for his-tagged sACE2

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Short GSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3 proHB-EGF CS

   Plasmid

   #11601

   Depositor

   Rosalyn Adam

   Article

   Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

   Insert

   HB-EGF (HBEGF Human)
   

   Use

   Tags

   His and myc

   Expression

   Mammalian

   Mutation

   Constitutively secreted HB-EGF: C-terminal amino …

   Promoter

   Available since

   April 26, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pcDNAZeo(-) LAMP2C

   Plasmid

   #89342

   Purpose

   Expresses human LAMP-2C in mammalian cells

   Depositor

   Janice Blum

   Article

   Perez et al J Immunol. 2016 Mar 15;196(6):2457-65. d

   Insert

   Lysosomal-associated membrane protein 2C (LAMP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SacI ML GFP Strand 11 Short

   Plasmid

   #164121

   Purpose

   Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

   Depositor

   Tito Calì, Marisa Brini

   Article

   Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

   Insert

   SacI ML GFP Strand 11 Short
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. F-MED26

    Plasmid

    #15367

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

    Insert

    MED26 (MED26 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-myc-his-BACH1 K52R

    Plasmid

    #17643

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    The lysine residue at position 52 was changed to …

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pY30

    Plasmid

    #84745

    Purpose

    Expresses huAsCpf1-P2A-puro and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huAsCpf1
    puromycin resistance gene
    

    Use

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Opto-M1R

    Plasmid

    #106069

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M1R; Opto-M1R; F11; 722)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-M1R (CHRM1 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CMV-mito-CAR-GECO1

    Plasmid

    #46022

    Purpose

    Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    CAR-GECO1
    

    Use

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

    Promoter

    CMV

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAAV_OHu19986C_myc tag

    Plasmid

    #183174

    Purpose

    used to make AAV vectors for hsTRPA1 tagged with myc

    Depositor

    Sreekanth Chalasani

    Article

    Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

    Insert

    hsTRPA1 (TRPA1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FLAG-MED7

    Plasmid

    #15416

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED7 (MED7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. Opto-A2AR

    Plasmid

    #106066

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-A2AR; Opto-A2AR; F8; 719)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-A2AR (ADORA2A Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Arrb1-RFP

    Plasmid

    #35401

    Depositor

    Robert Lefkowitz

    Article

    Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

    Insert

    beta-arrestin-1 (Arrb1 Rat)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA-dCas9-HA

    Plasmid

    #61355

    Purpose

    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HiBiT-FZD6

    Plasmid

    #195844

    Purpose

    Expresses human FZD6 with an N-terminal HiBiT; contains M345L

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD6 (FZD6 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    M345L

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. NSP6-Flag

    Plasmid

    #210345

    Purpose

    Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP6 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pY026

    Plasmid

    #84741

    Purpose

    Expresses huAsCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huAsCpf1
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mRFP-human APPL1

    Plasmid

    #22202

    Depositor

    Pietro De Camilli

    Article

    Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

    Insert

    Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA3-myc-his-BACH1 P47A

    Plasmid

    #17644

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    To generate the tumor-associated mutant, the prol…

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

25. OTR untagged

    Plasmid

    #208913

    Purpose

    encodes the human oxytocin receptor, without any tags

    Depositor

    Christian Gruber

    Article

    Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

    Insert

    OXTR (OXTR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TM5: 5x56, A218, Threonin instead of Alanin

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pY31

    Plasmid

    #84746

    Purpose

    Expresses huLbCpf1-P2A-puro and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    puromycin resistance gene
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FLAG-MED19

    Plasmid

    #15420

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED19 (Med19 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA3-sACE2(WT)

    Plasmid

    #145147

    Purpose

    Mammalian expression plasmid for soluble ACE2 (protease domain)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-c/o NL4-3 Env delta CT

    Plasmid

    #139285

    Purpose

    HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    codon optimized HIV-1 Env delta cytoplasmic tail
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of cytoplasmic tail after residue 710

    Promoter

    CMV IE

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Flag-V0D1

    Plasmid

    #210348

    Purpose

    Expresses ATP6V0D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    V-ATPase V0D1 (ATP6V0D1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge