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Showing: 5881 - 5920 of 8360 results

  1. pLenti6.2_mCherry_mito Grx1-roGFP2

    Plasmid
    #155044
    Purpose
    Fluorescent redox reporter
    Depositor
    Vanessa LaPointe
    Insert
    Glutaredoxin-1 (GLRX Human)
    Use
    Lentiviral
    Tags
    mitochondrial targeting sequence and roGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    155044-plasmid-map-sequence-id-311573

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  2. mDC-STAMP-Flag-Myc-CMV26

    Plasmid
    #73583
    Purpose
    Express mouse DC-STAMP in mammalian cells
    Depositor
    Paul Odgren
    Insert
    DC-STAMP (Dcstamp Mouse)
    Use
    Tags
    3x Flag and c-Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    73583-plasmid-map-sequence-id-136078

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  4. AAVS1-TRE-U2AF2-TurboID-HA-rtTA

    Plasmid
    #226997
    Purpose
    Integrative plasmid to express U2AF2-TurboID in mammalian cells
    Depositor
    Bruno Dallagiovanna
    Insert
    U2 small nuclear RNA auxiliary factor 2 (U2AF2 Human)
    Use
    Tags
    TurboID-HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    226997-plasmid-map-sequence-id-451922

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  6. hGli3 flag3x

    Plasmid
    #84921
    Purpose
    mammalian expression of Gli3
    Depositor
    Martin Fernandez-Zapico
    Insert
    Gli3 (GLI3 Human)
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84921-plasmid-map-sequence-id-421628

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  7. pBabe-Kras G12C

    Plasmid
    #58901
    Purpose
    Expresses human Kras with a G12C mutation
    Depositor
    Channing Der
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G12C mutation
    Promoter
    Available since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    58901-plasmid-map-sequence-id-242434

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  8. pCMV-Spike

    Plasmid
    #173921
    Purpose
    Expresses the original SARS-Cov-2 Spike Protein under a CMV Promoter
    Depositor
    Andreas Stuermer
    Insert
    SARS-2 Spike Protein (S Synthetic, Human, SARS-COV-2)
    Use
    Tags
    no tags, native protein
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    standard CMV
    Available since
    April 11, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    173921-plasmid-map-sequence-id-358849

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  9. pLEX307-APN-puro

    Plasmid
    #158454
    Purpose
    Constitutive lentiviral expression of human APN.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    APN (ANPEP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158454-plasmid-map-sequence-id-308145

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  10. psd44-Lactadherin46

    Plasmid
    #46830
    Purpose
    expression of human lactadherin in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    MFGE8 (MFGE8 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK promoter
    Available since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46830-plasmid-map-sequence-id-410396

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  12. K560GFP

    Plasmid
    #129761
    Purpose
    Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129761-plasmid-map-sequence-id-422377

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  14. Hs.NRAS G12D

    Plasmid
    #83176
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12D mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12D
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83176-plasmid-map-sequence-id-158139

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  15. p-dCas9-G9a-Hygro

    Plasmid
    #104408
    Purpose
    transient expression of dCas9-G9a fusion protein
    Depositor
    Stephan Beck
    Insert
    G9a (EHMT2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104408-plasmid-map-sequence-id-204469

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  16. CAMK2A

    Plasmid
    #38971
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CAMK2A (CAMK2A Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. 5'UTR CGG 99x FMR1

    Plasmid
    #63089
    Purpose
    Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS).
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63089-plasmid-map-sequence-id-104489

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  18. pAG426-GAL-Alpha-synuclein

    Plasmid
    #181715
    Purpose
    Galactose inducible expression of Alpha-synuclein
    Depositor
    Alejandro Chavez
    Insert
    Alpha-synuclein (SNCA Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    181715-plasmid-map-sequence-id-436188

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  19. pHN781

    Plasmid
    #178828
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Use
    Tags
    His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178828-plasmid-map-sequence-id-351929

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  20. pFRT-TODestRFP_HuD

    Plasmid
    #65766
    Purpose
    expresses RFP-tagged HuD in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL4 (ELAVL4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    V23M mutation in RFP
    Promoter
    CMV, 2x Tet
    Available since
    Aug. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. pCAG-GluA1-scFV-Halo

    Plasmid
    #230065
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin (CAG)
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230065-plasmid-map-sequence-id-460116

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  22. pLenti-mIFT88-EGFP

    Plasmid
    #149697
    Purpose
    Expresses mouse IFT88-EGFP in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    IFT88 (Ift88 Mouse)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    149697-plasmid-map-sequence-id-307392

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  24. pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)

    Plasmid
    #225433
    Purpose
    Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.
    Depositor
    Diego Orzaez
    Insert
    4xCBS:mDFR:Rep/RepA:Tnos
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225433-plasmid-map-sequence-id-447737

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  25. Hs.NRAS G13D

    Plasmid
    #83177
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G13D mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G13D
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83177-plasmid-map-sequence-id-158140

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  26. GST-thr-RAF1-RBD

    Plasmid
    #86033
    Purpose
    Expresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activation
    Depositor
    Dominic Esposito
    Insert
    RAF1 RBD (Ras-binding domain) (RAF1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Contains residues 1-149
    Promoter
    T7
    Available since
    Jan. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    86033-plasmid-map-sequence-id-170056

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  27. MDH2

    Plasmid
    #38792
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MDH2 (MDH2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. GPX3

    Plasmid
    #38899
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPX3 (GPX3 Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  29. pLEX307-DPP4-G418

    Plasmid
    #158453
    Purpose
    Constitutive lentiviral expression of human DPP4.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    DPP4 (DPP4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158453-plasmid-map-sequence-id-308173

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  30. pCIneo-hCHD7

    Plasmid
    #89458
    Purpose
    constitutive expression of human CHD7 in mammalian cells
    Depositor
    Peter Scacheri
    Insert
    CHD7 (CHD7 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89458-plasmid-map-sequence-id-176093

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  33. Hs.HRAS C186S

    Plasmid
    #83189
    Purpose
    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), C186S mutation
    Depositor
    Dominic Esposito
    Insert
    HRAS (HRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    C186S
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    83189-plasmid-map-sequence-id-158152

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  34. pET-Sac-Abeta(MC1-40)

    Plasmid
    #127152
    Purpose
    Expresses N-terminal cysteine Abeta(1-40)
    Depositor
    James Nowick
    Insert
    Abeta(MC1-40) (APP Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    127152-plasmid-map-sequence-id-247836

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  35. pLV-BDNF-pHluorin

    Plasmid
    #187193
    Purpose
    To express the mouse BDNF tagged with the FP pHluorin
    Depositor
    Pantelis Tsoulfas
    Insert
    BDNF (Bdnf Mouse)
    Use
    Lentiviral
    Tags
    pPHluorin
    Expression
    Mammalian
    Mutation
    The codon were optimized for better expression
    Promoter
    CMV
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    187193-plasmid-map-sequence-id-373713

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  37. AICSDP-82:SON-mEGFP

    Plasmid
    #133964
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human SON
    Depositor
    Allen Institute for Cell Science
    Insert
    SON Homology Arms with mEGFP-linker (SON Human)
    Use
    CRISPR
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Nov. 22, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    133964-plasmid-map-sequence-id-264342

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  38. NXPE1_pcDNA3.4-TOPO

    Plasmid
    #226841
    Purpose
    Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.
    Depositor
    Bert Vogelstein
    Insert
    NXPE1 (NXPE1 Human)
    Use
    Tags
    x6 HIS and an IL2 secretion signal both N termina…
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    226841-plasmid-map-sequence-id-456206

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  39. pT7-pelB

    Plasmid
    #157740
    Purpose
    empty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    PelB signal sequence and possible His6-tag (adds …
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157740-plasmid-map-sequence-id-334002

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  40. pLEX307-CTSB-puro

    Plasmid
    #158463
    Purpose
    Constitutive lentiviral expression of human CTSB.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSB (CTSB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158463-plasmid-map-sequence-id-308178

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Showing: 5881 - 5920 of 8360 results