We narrowed to 4,825 results for: Drosophila

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YW249_Pzipt17-loxP-H2BGFP-miniMOS

Plasmid

#196063

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

Depositor

Wenxing Yang

Publication

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

H2B::GFP
NeoR
Minimal Mos1
zipt17p::zipt17::zipt17 3UTR

Expression

Worm

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBelle_B

Plasmid

#145912

Purpose

Insect Expression of DmBelle

Depositor

Elisa Izaurralde

Insert

DmBelle (bel Fly)

Expression

Insect

Mutation

del 158-160 (GGG)

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1045C

Plasmid

#125005

Purpose

Expresses gRNAs targeting hid, eve, and wingless

Depositor

Omar Akbari

Publication

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCCr4

Plasmid

#79252

Purpose

Expresses EGFP-tagged DmCCr4 in S2 cells

Depositor

Elisa Izaurralde

Publication

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmCaf40 (twin Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.30 EC10-Fc-His

Plasmid

#72190

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Publication

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.30

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-Patronin CC 638–1457

Plasmid

#59051

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Publication

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC 776–1457

Plasmid

#59052

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Publication

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pET-21a-GFP-Patronin CC 1288–1457

Plasmid

#59055

Purpose

for E. coli expression of Patronin CC truncation

Depositor

Ron Vale

Publication

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

GFP

Expression

Bacterial

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pUAST-SIK3 T196A

Plasmid

#52974

Purpose

insect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)

Depositor

Marc Montminy, Biao Wang

Publication

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Tags

HA

Expression

Insect

Mutation

T196A LKB1 phosphorylation defective mutant

Promoter

Hsp70

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only
pAc5.1B-lambdaN-HA-DmMTR-4_AA

Plasmid

#148215

Purpose

Insect Expression of DmMTR-4

Depositor

Elisa Izaurralde

Insert

DmMTR-4 (l(2)35Df Fly)

Expression

Insect

Mutation

7 silent mutations compared to the sequence given…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.25 EC10-Fc-His

Plasmid

#72188

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.25 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Publication

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.25

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.25 EC10-AP-His

Plasmid

#72062

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.25 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Publication

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.25

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUASp-Flag-Myc-Qin-attB

Plasmid

#44208

Depositor

Phillip Zamore

Publication

Zhang et al Mol Cell. 2011 Nov 18;44(4):572-84. doi:

Insert

Qin full length (qin Fly)

Use

Fly transformation

Tags

Flag and Myc

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pETM-11-6His-DmBelle_1-694_B

Plasmid

#145951

Purpose

Bacterial Expression of DmBelle_1-694

Depositor

Elisa Izaurralde

Insert

DmBelle_1-694 (bel Fly)

Expression

Bacterial

Mutation

del 155-157, T530A and I685V

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmSmaug-V5His6_C

Plasmid

#146040

Purpose

Insect Expression of DmSmaug

Depositor

Elisa Izaurralde

Publication

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

DmSmaug (smg Fly)

Expression

Insect

Mutation

10 silent mutations and a deletion of the AA 961-…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPumilio_B

Plasmid

#145925

Purpose

Insect Expression of DmPumilio

Depositor

Elisa Izaurralde

Insert

DmPumilio (pum Fly)

Expression

Insect

Mutation

4 silent and two non silent mutations S322N and T…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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908E

Plasmid

#160238

Purpose

TtgO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

Depositor

Omar Akbari

Publication

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

Ttg Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter

Tags

T2A GFP

Expression

Insect

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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908G

Plasmid

#160239

Purpose

PipO(3x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

Depositor

Omar Akbari

Publication

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

Pip Operator (3x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter

Tags

T2A GFP

Expression

Insect

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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908J

Plasmid

#160242

Purpose

CymO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

Depositor

Omar Akbari

Publication

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

Cym Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter

Tags

T2A GFP

Expression

Insect

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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Opie2-NeoR[traF]+Opie2-PuroR[dsxM]

Plasmid

#131618

Purpose

Expresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.

Depositor

Omar Akbari

Publication

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Inserts

PuroR[dsxM]
NeoR[traF]

Expression

Insect

Promoter

Opie2

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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