-
Plasmid#117225PurposepET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.DepositorInsertCanthaxanthin Operon
UseTags34 amino acid N-terminal linker, Hexahistidine Ta…ExpressionBacterialMutationremoved Type IIS restriction sites within the bac…PromoterT7 RNA PolymeraseAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MCS-eDHFR(69K6)-EGFP
Plasmid#172101PurposeMammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFPDepositorInsert22aa-eDHFR(69K6)-EGFP
UseTagsExpressionMammalianMutationeDHFR(69K6): Hexalysine (K6) sequence is inserted…PromoterCMVAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGV3340
Plasmid#170278PurposeExpression of mutant HIV-2 RT p51 reverse transcriptaseDepositorInsertHIV-2 RT p51 (mutant)
UseTagsHexahistidineExpressionBacterialMutationPromoterT7-lacAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
WT N-His Ana GvpC in pET28a
Plasmid#153294PurposeExpresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coliDepositorInsertGas vesicle protein C (N-terminal his-tagged)
UseTagsHis-tagExpressionBacterialMutationAdded a hexahistidine tag after the methionine at…PromoterT7Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP6
Plasmid#71469PurposeEncodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 6 (SENP6 Human)
UseTagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
P15-pZE-SUMO-PiTTA
Plasmid#204630PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized piTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-BuTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
P17-pZE-SUMO-CsTTA
Plasmid#204631PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized csTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-CsTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
P18-pZE-SUMO-BuTTA
Plasmid#204632PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized buTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-BuTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
P24-pZE-SUMO-KaTTA
Plasmid#204633PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized kaTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-KaTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-LanCL2-H264A-C-Ter-His
Plasmid#154187PurposeTo express LanCL2-H264A in bacterial cellsDepositorInsertLanCL2
UseTagsHexahistidineExpressionBacterialMutationHis264->Ala264PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-LanCL1-H219A
Plasmid#154190PurposeTo express LanCL1-H219A in bacterial cellsDepositorInsertLanCL1
UseTagsHexahistidineExpressionBacterialMutationHis219->Ala219PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-LanCL1-C322A
Plasmid#154191PurposeTo express LanCL1-C322A in bacterial cellsDepositorInsertLanCL1
UseTagsHexahistidineExpressionBacterialMutationCys322->Ala322PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.MTF2
Plasmid#125169PurposeExpresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertMTF2 (MTF2 Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.PHF1
Plasmid#125167PurposeExpresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertPHF1 (PHF1 Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2X_MBP-CLmN-H6
Plasmid#234487PurposeMaltose binding protein N-terminally fused to His-tagged CLmN split inteinDepositorInsertN-terminal Fragment of the CLm Split Intein
UseTagsHexahistidine Tag and Maltose Binding Protein (MB…ExpressionBacterialMutationT69K, F75H, M118N within the N-terminal fragment …PromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
RP1B FUS 1-163
Plasmid#127192PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 (FUS Human)
UseTagshexahistidineExpressionBacterialMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGV3319
Plasmid#170277PurposeExpression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)DepositorInsertBst-LF D720A
UseTagsHexahistidineExpressionBacterialMutationD720APromoterT7-lacAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIC-His6-MBP-TEV-Rgs14
Plasmid#236395PurposeExpresses His6-MBP-TEV-Rgs14 in E. coliDepositorInsertRattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)
UseTagshexahistidine (H6) tag and maltose-binding protei…ExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_H6_His
Plasmid#201719PurposeExpression of nanobodyDepositorInsertVHH_H6 (S )
UseTagsHexahistidineExpressionBacterialMutationPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-LanCL2-C321A/C367A-C-Ter-His
Plasmid#154188PurposeTo express LanCL2-C321A/C367A in bacterial cellsDepositorInsertLanCL2
UseTagsHexahistidineExpressionBacterialMutationCys321->Ala321; Cys367->Ala367PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only