We narrowed to 2,441 results for: organization Addgene

1. pUF-LbCpf1-pre-gRNA

   Plasmid

   #137848

   Purpose

   LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

   Depositor

   Josh Beck

   Article

   Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

   Insert

   LbCpf1-NLS-FLAG and gRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. NPC1-CFP P692S

   Plasmid

   #58457

   Purpose

   expression of NPC1-CFP sterol sensing domain mutant

   Depositor

   Matthew Scott

   Article

   Ko et al Mol Biol Cell. 2001 Mar;12(3):601-14.

   Insert

   NPC1 (Npc1 Mouse)
   

   Use

   Tags

   ECFP and His6

   Expression

   Mammalian

   Mutation

   P692S sterol sensing domain mutant

   Promoter

   CMV

   Available since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pER-eZinCh-2

   Plasmid

   #70009

   Purpose

   expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

   Depositor

   Maarten Merkx

   Article

   Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

   Insert

   ER-eZinCh-2
   

   Use

   Tags

   KDEL sequence and PPI signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV 4xSTAR-mTFP-NLS-blast

   Plasmid

   #136257

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 4 repeats
   

   Use

   Lentiviral

   Tags

   mTFP-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKV016

   Plasmid

   #64767

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 3xT7-RFP tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM41-EcPPXc

   Plasmid

   #38329

   Depositor

   Florian Freimoser

   Article

   Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

   Insert

   E. coli polyphosphatase (ppx Escherichia coli)
   

   Use

   Tags

   His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   C-terminus of E. coli polyphosphatase (described …

   Promoter

   T7

   Available since

   Aug. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

7. KG#371

   Plasmid

   #110882

   Purpose

   Expresses the lysosome marker CTNS-1-mCherry cDNA in a subset of ventral cord cholinergic motor neurons

   Depositor

   Kenneth Miller

   Article

   Edwards et al Genetics. 2013 May;194(1):143-61. doi: 1

   Inserts

   unc-129 promoter
   CTNS-1a-mCherry cDNA
   unc-54 3' control region with 1 intron just upstream and 1 intron in control region
   

   Use

   Tags

   intron-mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Keratin 8 S73A

   Plasmid

   #18056

   Depositor

   M Bishr Omary

   Article

   Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

   Insert

   Keratin 8 (KRT8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S73A

   Promoter

   Available since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pKM263-EcPPXc

   Plasmid

   #38328

   Depositor

   Florian Freimoser

   Article

   Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

   Insert

   E. coli polyphosphatase (ppx Escherichia coli)
   

   Use

   Tags

   6xHIS, GST, and TEV

   Expression

   Bacterial

   Mutation

   C-terminus of E. coli polyphosphatase (described …

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNS3

    Plasmid

    #131770

    Purpose

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    

    Use

    Integration into δ-sites

    Tags

    Expression

    Yeast

    Mutation

    E490G to make Cry2 into Cry2Olig

    Promoter

    PADH1

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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