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Addgene

We narrowed to 4,976 results for: Apoe

Showing: 4531 - 4560 of 4976 results
  1. Venus-Bik-DD-pEGFP-C1

    Plasmid
    #177429
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.
    Depositor
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Threonine 33 and Serine 35 replaced with aspartic…
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pFGL1010

    Plasmid
    #119081
    Purpose
    Introducing ectopic integration at the defined fungal ILV2-locus, based on SRR (Sulfonylurea Resistance Reconstitution).
    Depositor
    Type
    Empty backbone
    Use
    Fungal expression (in magnaporthe oryzae)
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. Venus-Bik-GG153AA-pEGFP-C1

    Plasmid
    #177427
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.
    Depositor
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycines 153 and 154 mutated to alanine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. Venus-Bik-G154STOP-pEGFP-C1

    Plasmid
    #177428
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.
    Depositor
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycine 154 replaced with STOP codon
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pLX-TRV2

    Plasmid
    #180516
    Purpose
    Agrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and apolylinker
    Depositor
    Insert
    Tobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S CaMV
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pMSP1D1

    Plasmid
    #20061
    Purpose
    Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation
    Depositor
    Insert
    MSP1D1
    Use
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    deletion mutant (1-11) of MSP1; His-tag on N-term…
    Promoter
    Available since
    March 16, 2009
    Availability
    Academic Institutions and Nonprofits only
  7. pD-MSP1D1

    Plasmid
    #161607
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation
    Depositor
    Insert
    D-MSP1D1
    Use
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1; all tryptophans were replaced b…
    Promoter
    T7
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pGEMS-II-PEST

    Plasmid
    #204766
    Purpose
    GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)
    Depositor
    Insert
    EGFP-PEST-m6Alinker-DHFR
    Use
    Tags
    Expression
    Mutation
    mutated all RACs in EGFP to RGC/RCC
    Promoter
    CMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pBECKP-km

    Plasmid
    #117235
    Purpose
    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae
    Depositor
    Insert
    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 4531 - 4560 of 4976 results