We narrowed to 26,176 results for: vit;

1. pIA1385

   Plasmid

   #166862

   Purpose

   Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSOMA-NES

   Plasmid

   #118939

   Purpose

   Used to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-TIE-V5/HIS

   Plasmid

   #205612

   Purpose

   Expression of human TIE receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TIE receptor tyrosine kinase (TIE1 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIG-RARa-403-myc [TJ#344]

   Plasmid

   #16286

   Depositor

   Thomas Jessell

   Article

   Novitch et al Neuron. 2003 Sep 25. 40(1):81-95.

   Insert

   RAR alpha 403 (RARA Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   403 truncation. Dominant repressor derivative.

   Promoter

   Available Since

   Feb. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-DrFLINC-AKAR-Kras

   Plasmid

   #182334

   Purpose

   Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   DrFLINC-AKAR
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX

   Plasmid

   #188991

   Purpose

   A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Use

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   F163L, K249R, G328S, G381V, G429D, D480G

   Promoter

   CMV

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.Flag.HERC2-HECT-WT.6xHis

   Plasmid

   #39227

   Depositor

   Aziz Sancar

   Article

   Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

   Insert

   HERC2 HECT Domain (HERC2 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-hSyn-eGFP-SynaptoZip

   Plasmid

   #122525

   Purpose

   AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

   Depositor

   Antonio Malgaroli

   Article

   Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

   Insert

   eGFP-SynaptoZip (Vamp2 Rat)
   

   Use

   AAV

   Tags

   Myc and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

   Plasmid

   #188992

   Purpose

   A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Use

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   K109R, P245A, G328S, G381V, G429D

   Promoter

   CMV

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-mCherry-LKB1(KD)

    Plasmid

    #84640

    Purpose

    mCherry-tagged, LKB1(K78I); kinase-dead mutant

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    mCherry-LBK1(K78I) (STK11 Synthetic, Human)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Lysine 78 mutated to Isoleucine; kinase dead

    Promoter

    CMV

    Available Since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3 Flag MKK4beta

    Plasmid

    #15517

    Depositor

    Roger Davis

    Article

    Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

    Insert

    pCDNA3 Flag MKK4beta (MAP2K4 Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1-ROR1-V5/HIS

    Plasmid

    #205607

    Purpose

    Expression of human ROR1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    ROR1 receptor tyrosine kinase (ROR1 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3flag-RanGAPdeltaN

    Plasmid

    #25104

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    RanGAP1 deleted N-term domain (RANGAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-203.

    Promoter

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX

    Plasmid

    #188993

    Purpose

    A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Use

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    D201G, A258T, K327R, G328S, G381V, G429D, T450A

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-TNKS-2

    Plasmid

    #154149

    Purpose

    For making TNKS-2 RNA

    Depositor

    Carmen Williams

    Article

    Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

    Insert

    Tankyrase 2 (TNKS2 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    T7

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL1-mRFP1 gRNA

    Plasmid

    #117052

    Purpose

    In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    anti-mRFP1 SpCas9 gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KHBD00041

    Plasmid

    #34203

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG18455 (Optix Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA1-GATA4-opposite orientation

    Plasmid

    #51928

    Purpose

    for T7 directed antisense production of mouse GATA4

    Depositor

    Jeffery Molkentin

    Article

    Liang et al J Biol Chem. 2001 Aug 10;276(32):30245-5

    Insert

    GATA4 reverse orientation (Gata4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only

19. mutpRL-TK 3xUTR

    Plasmid

    #40758

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    3 copies of the CXCR4 small RNA target site
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

20. KHBD00259

    Plasmid

    #34391

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG31240 (repo Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only