We narrowed to 22,334 results for: CRISPR

1. pS648∙CsR

   Plasmid

   #197860

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Gm resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PEM7

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pC014 - LwCas13a-msfGFP

   Plasmid

   #91902

   Purpose

   Expresses active LwCas13a for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR

   Tags

   NLS and msfGFP-NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pS548∙CsR

   Plasmid

   #197859

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Tc resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PEM7

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pspCas9-HF-tetra-com-vector

   Plasmid

   #132555

   Purpose

   Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Inserts

   High fidelity Cas9
   sgRNA scaffold with com replacing the Tetraloop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCas9D10A_GFP

   Plasmid

   #44720

   Purpose

   Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9D10A-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   D10A mutation- nickase mutant

   Promoter

   CAG

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-CASI-Cas9C-P2A-turboGFP

   Plasmid

   #80942

   Purpose

   Expresses Cas9C in mammalian cells; co-expresses turboGFP.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-MH6-EsCas13d

   Plasmid

   #108303

   Purpose

   Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Insert

   EsCas13d
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSpCas9n(BB)-2A-Puro (PX462)

   Plasmid

   #48141

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pYPQ131B2.0

   Plasmid

   #99885

   Purpose

   Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU3

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ132B2.0

    Plasmid

    #99888

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU3

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lenti-CDDR

    Plasmid

    #149355

    Purpose

    CRISPR/Cas9-based dual-fluorescent DSB repair reporter

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    CDDR Reporter
    Blasticidin resistance
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hPGK

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-gRNA

    Plasmid

    #53062

    Purpose

    Transcription of guide RNA (gRNA) in wheat cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    wheat U6 promoter - gRNA
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    wheat U6

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AsCas12a-6xNLS-E174R/S542R (pRG232)

    Plasmid

    #149723

    Purpose

    Lentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance gene

    Depositor

    Junwei Shi

    Article

    Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. VCP F12.4 gRNA

    Plasmid

    #90939

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human VCP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    VCP (Guide Designation F12.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. VCP F11.4 gRNA

    Plasmid

    #90938

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human VCP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    VCP (Guide Designation F11.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-MH6-RspCas13d

    Plasmid

    #108305

    Purpose

    Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Insert

    RspCas13d
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. BRCA2 A12.2 gRNA

    Plasmid

    #90551

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA2 (Guide Designation A12.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAS9id

    Plasmid

    #141251

    Purpose

    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ133B2.0

    Plasmid

    #99892

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU3

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO5.sgRNA.EFS.tRFP657

    Plasmid

    #57824

    Purpose

    Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    tagRFP657
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge