We narrowed to 4,048 results for: gast

1. HA-dora[B]_rescue_construct

   Plasmid

   #190638

   Purpose

   Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Use

   Tags

   3xHA

   Expression

   Insect

   Mutation

   Changed threonine 295 to methionine

   Promoter

   Actin-5c

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. dora[A]_rescue_construct

   Plasmid

   #190639

   Purpose

   Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Truncated to contain amino acids 1-945

   Promoter

   Actin-5c

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HA-dora[A]_rescue_construct

   Plasmid

   #190640

   Purpose

   Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Use

   Tags

   3xHA

   Expression

   Insect

   Mutation

   Truncated to contain amino acids 1-945

   Promoter

   Actin-5c

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBABR mCherry-Bcd-LEXY

   Plasmid

   #182596

   Purpose

   Drosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.

   Depositor

   Jared Toettcher

   Article

   Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

   Insert

   Bcd (bcd Fly)
   

   Use

   Tags

   LEXY and mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABR Bcd-LEXY

   Plasmid

   #182594

   Purpose

   Drosophila integration plasmid expressing Bcd-LEXY fusion protein under the maternal tubulin promoter.

   Depositor

   Jared Toettcher

   Article

   Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

   Insert

   Bicoid (bcd Fly)
   

   Use

   Tags

   LEXY

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuetG-DmXRN1_1312-1355-DmDCP1_1-127_N

   Plasmid

   #147054

   Purpose

   Bacterial Expression of DmXRN1_1312-1355-DmDCP1_1-127

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   DmXRN1_1312-1355-DmDCP1_1-127 (pcm Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSFDuetG-DmXRN1_1323-1355-DmDCP1_1-127_N

   Plasmid

   #147055

   Purpose

   Bacterial Expression of DmXRN1_1323-1355-DmDCP1_1-127

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   DmXRN1_1323-1355-DmDCP1_1-127 (pcm Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSFDuetG-DmXRN1_1323-1383-DmDCP1_1-127_N

   Plasmid

   #147056

   Purpose

   Bacterial Expression of DmXRN1_1323-1383-DmDCP1_1-127

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   DmXRN1_1323-1383-DmDCP1_1-127 (pcm Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1B-HA-DmDCP1-N41AS100A-dsRNAres_P

   Plasmid

   #147215

   Purpose

   Insect Expression of DmDCP1-N41AS100A-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   DmDCP1-N41AS100A-dsRNAres (Dcp1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-HA-DmDCP1-Y34F-dsRNAres_P

    Plasmid

    #147217

    Purpose

    Insect Expression of DmDCP1-Y34F-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    DmDCP1-Y34F-dsRNAres (Dcp1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. puc19_N_tag

    Plasmid

    #186650

    Purpose

    Empty donor plasmid.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    N-terminal 3xFlag-3xHA Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTL464

    Plasmid

    #69881

    Purpose

    a dCirl transcriptional reporter allele that contains an optimized gal4.2::p65 cassette at the start codon of the genomic dCirl ORF

    Depositor

    Robert Kittel, Tobias Langenhan

    Article

    Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

    Insert

    CIRL (Cirl Fly)
    

    Use

    Phic31-integration vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-EGFP-DmCaf40-A253FY208A_X

    Plasmid

    #147973

    Purpose

    Insect Expression of DmCaf40-A253FY208A

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmCaf40-A253FY208A (Rcd-1 Fly)
    

    Use

    Tags

    EGFP (A179V)

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-lambdaN-HA-Dm4E-T_1-511-LLFWKtoAAAAA-V5His6_Z

    Plasmid

    #148160

    Purpose

    Insect Expression of Dm4E-T_1-511-LLFWKtoAAAAA

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    Dm4E-T_1-511-LLFWKtoAAAAA (4E-T Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    alias: 4E-T; one non silent mutation L127S, annot…

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-2T-CBP(1-287)

    Plasmid

    #182839

    Purpose

    CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    CBP1-287
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET11-PC(75-390)-H6

    Plasmid

    #183781

    Purpose

    Express PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assays

    Depositor

    Peter Harte

    Article

    Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

    Insert

    Polycomb (w Fly)
    

    Use

    Tags

    6 His tag at the C-terminus

    Expression

    Bacterial

    Mutation

    6 His tag at the C-terminus

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-EGFP-DmHelz-E958Q_AH

    Plasmid

    #148884

    Purpose

    Insect Expression of DmHelz-E958Q

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    DmHelz-E958Q (CG9425 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent an two non silent G1374S, R2076G mutat…

    Promoter

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149427

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[TraB, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pnEA-NpM-DmThor_50-83-GB1_Z

    Plasmid

    #148180

    Purpose

    Bacterial Expression of DmThor_50-83

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor_50-83 (Thor Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-EGFP-DmHelz_1213-2103_AH

    Plasmid

    #148883

    Purpose

    Insect Expression of DmHelz_1213-2103

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    DmHelz_1213-2103 (CG9425 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent an two non silent G1374S, R2076G mutat…

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge