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Pooled Library#64750PurposeA genome-wide CRISPR library covering 13,501 genes (98.8% of the Drosophila melanogaster genome), among which 8,989 genes are targeted by three or more independent single guide RNAs (sgRNAs).DepositorExpressionInsectSpeciesDrosophila melanogaster, Streptococcus pyogenes, and SyntheticUseCRISPRAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only
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pcDNA GASTM-3-RVG-10-Lamp2b-HA
Plasmid#71295PurposeEncodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsGASTM mutated glycosylation motif, HA, Lamp2 sig…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2 mouse twisted gastrulation
Plasmid#25778DepositorInserttwisted gastrulation (Twsg1 Mouse)
UseTagsFlag and chordin signal peptideExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCS2 xenopus twisted gastrulation
Plasmid#24974DepositorInserttwisted gastrulation (Twsg1 Mouse)
UseTagsFLAG and chordin signal peptideExpressionMammalianMutationPromoterAvailable sinceJune 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEM mouse twisted gastrulation
Plasmid#25780DepositorInserttwisted gastrulation (Twsg1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 xenopus twisted gastrulation
Plasmid#24680DepositorInserttwisted gastrulation (twsg1 Frog)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
LOC5567066 Gastrin-releasing peptide receptor
Plasmid#115372PurposeExpresses Ae. aegypti receptor in mammalian cellsDepositorInsertLOC5567066 Gastrin-releasing peptide receptor (LOC5567066 Aedes aegypti)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GASTM-3-RVG-10-Lamp2b-HA
Plasmid#71295PurposeEncodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsGASTM mutated glycosylation motif, HA, Lamp2 sig…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2 mouse twisted gastrulation
Plasmid#25778DepositorInserttwisted gastrulation (Twsg1 Mouse)
UseTagsFlag and chordin signal peptideExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCS2 xenopus twisted gastrulation
Plasmid#24974DepositorInserttwisted gastrulation (Twsg1 Mouse)
UseTagsFLAG and chordin signal peptideExpressionMammalianMutationPromoterAvailable sinceJune 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEM mouse twisted gastrulation
Plasmid#25780DepositorInserttwisted gastrulation (Twsg1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 xenopus twisted gastrulation
Plasmid#24680DepositorInserttwisted gastrulation (twsg1 Frog)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
LOC5567066 Gastrin-releasing peptide receptor
Plasmid#115372PurposeExpresses Ae. aegypti receptor in mammalian cellsDepositorInsertLOC5567066 Gastrin-releasing peptide receptor (LOC5567066 Aedes aegypti)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 Drosophila melanogaster CBS-pair domain
Plasmid#166436PurposeExpress pGEX-6P-1 Drosophila melanogaster CBS-pair domainDepositorInsertDrosophila melanogaster CBS-pair domain (Cbs Fly)
UseTagsGSTExpressionBacterialMutationPromoterTac, LacI, AmpAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)
Plasmid#166418PurposeExpress pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)DepositorInsertDrosophila melanogaster PRL (Prl Fly)
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET23a-mUbc9
Plasmid#53137PurposeE.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)DepositorInsertUbc9 (Ube2i Mouse)
UseTagsuntaggedExpressionBacterialMutationprotein sequence is identical to humanPromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYM70
Plasmid#119922PurposeCaHygB plasmids that facilitate genetic manipulations in the fungal pathogenDepositorInsertCaHygB
UseTagsCaHygBExpressionMutationPromoterTEF2Available sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX3X-RanBP2dFG
Plasmid#53120PurposeE.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activityDepositorInsertRanBP2 fragment (RANBP2 Human)
UseTagsGSTExpressionBacterialMutationfragment (amino acids 2553-2838)PromotertacAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET23a-mUbc9
Plasmid#53137PurposeE.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)DepositorInsertUbc9 (Ube2i Mouse)
UseTagsuntaggedExpressionBacterialMutationprotein sequence is identical to humanPromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET11d-hUba2
Plasmid#53136PurposeE.coli expression of full length untagged human Uba2 (SAE2)DepositorInsertUBA2 (UBA2 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only