We narrowed to 4,078 results for: gast

1. Oxford Drosophila melanogaster Genome-wide CRISPR Library

   Pooled Library

   #64750

   Purpose

   A genome-wide CRISPR library covering 13,501 genes (98.8% of the Drosophila melanogaster genome), among which 8,989 genes are targeted by three or more independent single guide RNAs (sgRNAs).

   Depositor

   Ji-Long Liu

   Article

   Bassett et al J Genet Genomics. 2015 Jun 20;42(6):301-

   Expression

   Insect

   Species

   Drosophila melanogaster, Streptococcus pyogenes, and Synthetic

   Use

   CRISPR

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA GASTM-3-RVG-10-Lamp2b-HA

   Plasmid

   #71295

   Purpose

   Encodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Tags

   GASTM mutated glycosylation motif, HA, Lamp2 sig…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2 mouse twisted gastrulation

   Plasmid

   #25778

   Depositor

   Edward De Robertis

   Insert

   twisted gastrulation (Twsg1 Mouse)
   

   Tags

   Flag and chordin signal peptide

   Expression

   Mammalian

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pCS2 xenopus twisted gastrulation

   Plasmid

   #24974

   Depositor

   Edward De Robertis

   Article

   Larrain et al Development. 2001 Nov . 128(22):4439-47.

   Insert

   twisted gastrulation (Twsg1 Mouse)
   

   Tags

   FLAG and chordin signal peptide

   Expression

   Mammalian

   Available Since

   June 18, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pGEM mouse twisted gastrulation

   Plasmid

   #25780

   Depositor

   Edward De Robertis

   Insert

   twisted gastrulation (Twsg1 Mouse)
   

   Expression

   Bacterial

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3 xenopus twisted gastrulation

   Plasmid

   #24680

   Depositor

   Edward De Robertis

   Article

   Oelgeschlager et al Nature. 2000 Jun 15. 405(6788):757-63.

   Insert

   twisted gastrulation (twsg1 Frog)
   

   Expression

   Mammalian

   Available Since

   June 7, 2010

   Availability

   Academic Institutions and Nonprofits only

7. LOC5567066 Gastrin-releasing peptide receptor

   Plasmid

   #115372

   Purpose

   Expresses Ae. aegypti receptor in mammalian cells

   Depositor

   Leslie Vosshall

   Article

   Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

   Insert

   LOC5567066 Gastrin-releasing peptide receptor (LOC5567066 Aedes aegypti)
   

   Expression

   Mammalian

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-6P-1 Drosophila melanogaster CBS-pair domain

   Plasmid

   #166436

   Purpose

   Express pGEX-6P-1 Drosophila melanogaster CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Drosophila melanogaster CBS-pair domain (Cbs Fly)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   Tac, LacI, Amp

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)

   Plasmid

   #166418

   Purpose

   Express pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Drosophila melanogaster PRL (Prl Fly)
   

   Tags

   N-terminal His-tag

   Expression

   Bacterial

   Mutation

   Deleted nucleotides coding for 4 C-terminal amino…

   Promoter

   AmpR, lac1

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET23a-mUbc9

    Plasmid

    #53137

    Purpose

    E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    Ubc9 (Ube2i Mouse)
    

    Tags

    untagged

    Expression

    Bacterial

    Mutation

    protein sequence is identical to human

    Promoter

    T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYM70

    Plasmid

    #119922

    Purpose

    CaHygB plasmids that facilitate genetic manipulations in the fungal pathogen

    Depositor

    Brian Wong

    Article

    Basso LR et al Yeast. 2010 Dec;27(12):1039-48. doi: 10.

    Insert

    CaHygB
    

    Tags

    CaHygB

    Promoter

    TEF2

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX3X-RanBP2dFG

    Plasmid

    #53120

    Purpose

    E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    RanBP2 fragment (RANBP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    fragment (amino acids 2553-2838)

    Promoter

    tac

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11d-hUba2

    Plasmid

    #53136

    Purpose

    E.coli expression of full length untagged human Uba2 (SAE2)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    UBA2 (UBA2 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-His-hAos1

    Plasmid

    #53135

    Purpose

    E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    Aos1 (SAE1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET11a-SUMO1

    Plasmid

    #53138

    Purpose

    E.coli expression of untagged mature human SUMO1 (amino acids 1-97)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    SUMO1 (SUMO1 Human)
    

    Expression

    Bacterial

    Mutation

    mature form (amino acids 1-97)

    Promoter

    T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. phGR-SB

    Plasmid

    #133409

    Purpose

    overexpression of a human glucocorticoid receptor in mammalian cells

    Depositor

    Andrew Cato

    Article

    Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

    Insert

    NR3C1 (NR3C1 Human)
    

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. GR A458T

    Plasmid

    #133410

    Purpose

    overexpression of a mutant human glucocorticoid receptor in mammalian cells

    Depositor

    Andrew Cato

    Article

    Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

    Insert

    NR3C1 (NR3C1 Human)
    

    Expression

    Mammalian

    Mutation

    A458T

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. hGR-Dx

    Plasmid

    #133408

    Purpose

    overexpression of a mutant human glucocorticoid receptor in mammalian cells

    Depositor

    Andrew Cato

    Article

    Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

    Insert

    NR3C1 (NR3C1 Human)
    

    Expression

    Mammalian

    Mutation

    N454D, A458T, R460D, D462C

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUG-natNT2

    Plasmid

    #110922

    Purpose

    expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    nat
    

    Expression

    Bacterial and Yeast

    Promoter

    AgTEF1

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUDP082

    Plasmid

    #103875

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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