We narrowed to 4,353 results for: erf

1. CMV-d2eGFP-16pf

   Plasmid

   #21969

   Depositor

   Phil Sharp

   Article

   Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

   Insert

   CMV d2eGFP miR-16 perfect
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

2. C114m

   Plasmid

   #121013

   Purpose

   MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   dCas9 with no bbsI or BsaI sites
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTH341-SUP45(139-437)-GalBD

   Plasmid

   #29741

   Depositor

   Tobias von der Haar

   Insert

   SUP45 (SUP45 Budding Yeast)
   

   Use

   Tags

   Gal Activation domain and Myc

   Expression

   Yeast

   Mutation

   Codons 139-437 of the yeast SUP45 gene only

   Promoter

   Available Since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTH342-SUP45(276-437)-GalBD

   Plasmid

   #29742

   Depositor

   Tobias von der Haar

   Insert

   SUP45 (SUP45 Budding Yeast)
   

   Use

   Tags

   Gal Activation domain and Myc

   Expression

   Yeast

   Mutation

   Codons 276-437 of the yeast SUP45 gene only

   Promoter

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLPC hRap1-FL

   Plasmid

   #12542

   Depositor

   Titia de Lange

   Article

   Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

   Insert

   Repressor Activator Protein 1 (TERF2IP Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pUC8 mouse IFN-gamma

   Plasmid

   #10955

   Depositor

   Howard Young

   Insert

   interferon-gamma (Ifng Mouse)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HA-p48

   Plasmid

   #11614

   Depositor

   Steven Johnson

   Insert

   Interferon-Stimulated Gene Factor 3 (IRF9 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

8. CMV-dCas9_puroR

   Plasmid

   #219825

   Purpose

   CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)

   Depositor

   Philipp Maass

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   CMV

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. J-IRF5-5

   Plasmid

   #166525

   Purpose

   scFv of a human scaffold targeting Interferon regulatory factor 5. Antigen coverage aa E232-L434 of 498

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. UKC1620-SUP35wt

    Plasmid

    #29730

    Depositor

    Tobias von der Haar

    Insert

    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Wild type gene including genomic sequence 955 bp …

    Promoter

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

11. PDZD3

    Plasmid

    #38880

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PDZD3 (PDZD3 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pGustiv

    Plasmid

    #240335

    Purpose

    Expresses BK polyomavirus genotype IV capsid protein VP1 in yeast. Expression of VP1 (and a GFP reporter) is induced by maltose.

    Depositor

    Christopher Buck

    Inserts

    BKV-IV VP1
    Superfold GFP
    Formaldehyde dehydrogenase 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    FDH1, MAL31, and MAL32

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. dCas9-miniTurbo

    Plasmid

    #219822

    Purpose

    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    EF1a

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

    Plasmid

    #175573

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid

    #196712

    Purpose

    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-KRAB(ZIM3)-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

    Plasmid

    #175574

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOpen-GBDpol (exo)

    Plasmid

    #165572

    Purpose

    Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid

    #102244

    Purpose

    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter

    Depositor

    Jacob Corn

    Insert

    dCas9-HA-BFP-KRAB-NLS
    

    Use

    CRISPR and Lentiviral

    Tags

    HA-TagBFP-KRAB-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-GFP-p48

    Plasmid

    #11616

    Depositor

    Steven Johnson

    Insert

    Interferon-Stimulated Gene Factor 3 (IRF9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

20. XClone-Cas9-Cronus-T2A-BFP

    Plasmid

    #141209

    Purpose

    Tunable and temporal expression control of Cas9

    Depositor

    Rory Johnson

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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