We narrowed to 15,088 results for: pET

1. Mucin MUC1-5TR-pET15b

   Plasmid

   #174694

   Purpose

   Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

   Depositor

   Jean Gariepy

   Article

   Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

   Insert

   MUC1-TR
   

   Tags

   hexahistidine (HIS) tag

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 Promoter

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. His-SUMO-hDDI2-D252N_pET11a

   Plasmid

   #169122

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hDDI (DDI2 Human)
   

   Expression

   Bacterial

   Mutation

   D252N

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a-GFP-AURKA_K162M-mCherry

   Plasmid

   #99881

   Purpose

   Expression of GFP-AURKA K162M-mCherry for protein purification in bacteria

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP and mCherry

   Expression

   Bacterial

   Mutation

   K162M (kinase-dead)

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-mEB1-eGFP-6xHis

   Plasmid

   #130981

   Purpose

   Bacterial expression of mouse EB1-GFP-6xHis for protein purification.

   Depositor

   Anne Straube

   Article

   Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

   Insert

   EB1-GFP-6xHis (Mapre1 Mouse)
   

   Tags

   eGFP-6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET 15b-UbcH5a/2417

   Plasmid

   #11472

   Depositor

   Allan Weissman

   Article

   Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

   Insert

   UbcH5a (UBE2D1 Human)
   

   Expression

   Bacterial

   Mutation

   n/a

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pET16b-Rnl2(1-249)K227Q

   Plasmid

   #14072

   Depositor

   Thomas Tuschl

   Insert

   Rnl2(1-249)
   

   Tags

   His (10x)

   Expression

   Bacterial

   Mutation

   changed Lys227 to Glutamine Gly112 is actually C…

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pET28_mouse-Caspase-11_enzymatic-domain

   Plasmid

   #183382

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-11 (AA100-373) (Casp4 Mouse)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-51b(+)-cpHaloTag141/145

   Plasmid

   #194660

   Purpose

   Protein production of circular permuted HaloTag (cpHaloTag141/145) in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   cpHaloTag141/145
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-51b(+)-cpHaloTag154/156

   Plasmid

   #194661

   Purpose

   Protein production of circular permuted HaloTag (cpHaloTag154/156) in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   cpHaloTag154/156
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. [pSK104] pET28 SLC38A9N-His

    Plasmid

    #136142

    Purpose

    bacterial expression of SLC38A9 N term fragment (1-119)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    SLC38A9 N term fragment (1-119) (SLC38A9 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-A3A(N57Q)-BE3

    Plasmid

    #158157

    Purpose

    T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

    Depositor

    Keith Joung

    Article

    Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

    Insert

    6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
    

    Tags

    6xHis tag on N-terminus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

    Plasmid

    #198751

    Purpose

    Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - LargeBiT
    

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

    Plasmid

    #198750

    Purpose

    Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - SmallBiT
    

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET16b-Trx CC32/35SS

    Plasmid

    #36946

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin 1 (TXN Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    both active site cysteines (32,35) mutated to ser…

    Promoter

    T7

    Available Since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pF709-pET28a-Hs-FL-ETFbeta-NHis wt

    Plasmid

    #85110

    Purpose

    expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length ETFbeta (ETFB Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a(+) 6xHis-3xFlag-IFIT5

    Plasmid

    #53561

    Purpose

    bacterial expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Tags

    3xFLAG, 6xHIS, and TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pET28-Nsp14-TEV-6His

    Plasmid

    #154952

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp14-TEV-6His (ORF1ab SARS-CoV-2)
    

    Tags

    TEV-6His

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pET51b(+)_LAMA-F98 (for GFP)

    Plasmid

    #130714

    Purpose

    Plasmid encoding the bacterial expression of LAMA-F98 for GFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    LAMA-F98
    

    Tags

    Hisx10

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. 3521 pET15b Bid p15

    Plasmid

    #8782

    Depositor

    Stanley Korsmeyer

    Article

    Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

    Insert

    Bid p15 (Bid Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pET_SZ1-RGG-GFP-RGG

    Plasmid

    #124937

    Purpose

    Expression of SZ1-RGG-GFP-RGG

    Depositor

    Daniel Hammer, Matthew Good, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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