We narrowed to 15,747 results for: pET

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pET SUMO NL-FKBP-Cas9

Plasmid

#162730

Purpose

Bacterial expression, protein expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Tags

6xHis and SUMO

Expression

Bacterial

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-Strep-TEV-Nsp14

Plasmid

#154955

Purpose

Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

Depositor

Justin Kollman

Insert

Strep-TEV-Nsp14 (ORF1ab SARS-CoV-2)

Tags

Strep-TEV

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET302-6His-dCas9-Halo

Plasmid

#72269

Purpose

Encodes a Halo-labeled fusion of nuclease deficient Cas9

Depositor

Timothée Lionnet

Article

Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

Insert

His6-dCas9-Halo

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-His-TEV-NQO1

Plasmid

#119058

Purpose

For expression in E.coli and affinity purification of N-terminally His tagged NQO1

Depositor

Yosef Shaul

Article

Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

6His, TEV site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymYPET-URA3

Plasmid

#168056

Purpose

Replaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FP

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET

Tags

ymYPET

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET-45b-mEGFP-HMGB1-WT

Plasmid

#194543

Purpose

Bacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Bacterial

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-HaloTag7

Plasmid

#167266

Purpose

Overexpression of HaloTag7 in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HaloTag7

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET30b-5M SrtA with TEV site

Plasmid

#86962

Purpose

Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

Depositor

Ron Bose

Article

Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

Insert

S.aureus SrtA 5M

Tags

6x Histidine tag and TEV site

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop A

Plasmid

#171779

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop A

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Val2…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-his6-HA3-SNAPf

Plasmid

#188941

Purpose

T7 expression vector for making his6- and HA3-tagged SNAP protein

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-SNAPf

Expression

Bacterial

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-his6-HA3-HALO

Plasmid

#188943

Purpose

T7 expression vector for making his6- and HA3-tagged HALO protein

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-HALO

Expression

Bacterial

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-NanoBondy 2H5(R72C)

Plasmid

#247051

Purpose

For expression of covalently reactive anti-CD45 2H5-derived NanoBondy (R72C clamp site, 9 residue linker) in bacterial cytoplasm

Depositor

Omer Dushek

Article

Vankayala et al Bioconjug Chem. 2026 Feb 18;37(2):281-29

Insert

NanoBondy 2H5(R72C)-Ctag

Tags

C-tag and SpyTag003

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pET28b(+)_S. cerevisiae Hbs1(1-611)

Plasmid

#39336

Depositor

SeWon Suh

Insert

Elongation factor 1 alpha-like protein

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pET30-ARH3-His-Sumo-HA

Plasmid

#111578

Purpose

Expresses ARH3 (amino acids 14-363) in E. coli

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

ARH3 (ADPRS Human)

Tags

6xHis-Sumo and HA

Expression

Bacterial

Mutation

deleted amino acids 1-13

Promoter

T7

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-dspB(E184Q)-6xHis

Plasmid

#175802

Purpose

Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-BS2-TRFLE

Plasmid

#247993

Purpose

BS2-TRFLE bacterial expression plasmid

Depositor

Jeffrey Martell

Article

Ogorek et al ACS Cent. Sci. 2026,

Insert

BS2-TRFLE Esterase

Tags

His Tag

Expression

Bacterial

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pET14b His Rab8a Q67L

Plasmid

#186014

Purpose

His Rab8a Q67L

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

His Rab8a Q67L (RAB8A Human)

Expression

Bacterial

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET-His6MBP-A3A-Gly6His6

Plasmid

#187822

Purpose

Expression human APOBEC3A in E.coli.

Depositor

Hao Wu

Article

Fabyanic et al Nat Biotechnol. 2023 Aug 28. doi: 10.103

Insert

APOBEC3A (APOBEC3A Human)

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

none

Promoter

T7 promoter (on the backbone)

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos15)GFP

Plasmid

#89248

Purpose

[Edit] Expresses GFP with +15 theoretical charge in E.Coli

Depositor

David Liu

Article

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos15)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a SnoopTag-mEGFP-SpyTag

Plasmid

#72325

Purpose

Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SnoopTag-mEGFP-SpyTag

Tags

N-terminal His6 tag

Expression

Bacterial

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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