We narrowed to 14,886 results for: pET

1. pET28a-SENP8 (full length))

   Plasmid

   #16361

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP8 full length (SENP8 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   human SENP8 full length, see Depositor Comments

   Promoter

   Available since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-His6-Ubc9-K14R

   Plasmid

   #185759

   Purpose

   Bacterial expression of His6-Ubc9-K14R

   Depositor

   Jeffrey Bode

   Article

   Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Use

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   K14R

   Promoter

   T7

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-FLAG-SpCas9-HF1

   Plasmid

   #126770

   Purpose

   Expression of increased fidelity SpCas9-HF1 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETDuet RagA(D130N)-RagC

   Plasmid

   #99642

   Purpose

   Bacterial expression of RagA(D130N)-RagC

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   D130N

   Promoter

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-TRE-YPet-WPRE

   Plasmid

   #193342

   Purpose

   AAV-mediated expression of YPet under the TRE promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

   Insert

   YPet
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET151-D-TOPO-PARP14-SΔK

   Plasmid

   #216658

   Purpose

   Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

   Depositor

   Herwig Schüler

   Article

   Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

   Insert

   PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
   

   Use

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   Deletion starting from Q1390 to D1448 and substit…

   Promoter

   T7

   Available since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet RagA(T21N)-RagC

   Plasmid

   #99644

   Purpose

   Bacterial expression of RagA(T21N)-RagC

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   T21N

   Promoter

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET23-PBD(65-150)-N-Cys

   Plasmid

   #13722

   Depositor

   Klaus Hahn

   Article

   Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

   Insert

   PBD(65-150)-N-Cys (PAK1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Met-Cys-Gly-PBD(65-150)-

   Promoter

   Available since

   March 28, 2007

   Availability

   Academic Institutions and Nonprofits only

9. PET28a-cpLumiLuc-Rapamycin sensor

   Plasmid

   #186292

   Purpose

   Expression Rapamycin sensor in e coli

   Depositor

   Kirill Alexandrov

   Article

   Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

   Insert

   cpLumiLuc-Rapamycin sensor
   

   Use

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-TGFα-GSY-SPM

    Plasmid

    #163615

    Purpose

    Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    TGFa-GSY-SPM (TGFA Human, Synthetic)
    

    Use

    Tags

    C-tag and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-KIX (586-672)

    Plasmid

    #99336

    Purpose

    Bacterial expression of mouse CBP KIX domain

    Depositor

    Peter Wright

    Article

    Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

    Insert

    CBP KIX (586-672) (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET22b 6His SpyCatcher-GRFT

    Plasmid

    #204502

    Purpose

    Bacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linker

    Depositor

    Urartu Seker

    Article

    Ozkul et al Adv Mater Interfaces. 2022 Sep 13:220112

    Insert

    GRFT
    

    Use

    Synthetic Biology

    Tags

    6xHis and SpyCatcher

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET22b-mEB2-eGFP-6xHis

    Plasmid

    #130982

    Purpose

    Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB2-GFP-6xHis (Mapre2 Mouse)
    

    Use

    Tags

    eGFP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-6His-SUMO-Nsp14

    Plasmid

    #154956

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET28b-MBP-TEV-PWWP_DNMT3A

    Plasmid

    #186970

    Purpose

    Bacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.

    Depositor

    Brian Liau

    Article

    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

    Insert

    DNMT3A PWWP domain (DNMT3A Human, Synthetic)
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    PWWP domain (residues 278–427)

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a_DmKHC[1-421]-SNAP-6xHis

    Plasmid

    #196975

    Purpose

    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

    Depositor

    Lukas Kapitein

    Article

    Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Use

    Tags

    6xHis and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM10 with rat FL Annexin A11

    Plasmid

    #164496

    Purpose

    For bacterial expression of rat His-tagged FL Annexin A11

    Depositor

    Anni Vedeler

    Article

    Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

    Insert

    rat FL Annexin A11 (Anxa11 Rat)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-SENP5 (catalytic domain)

    Plasmid

    #16358

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP5 catalytic domain (SENP5 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 567 to 755 catalytic domain of human S…

    Promoter

    Available since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pETconNK-TEM1(S70A,D179G)

    Plasmid

    #81166

    Purpose

    pETconNK with TEM1(S70A,D179G) beta-lactamase

    Depositor

    Timothy Whitehead

    Article

    Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

    Insert

    TEM-1 beta-lactamase
    

    Use

    Tags

    c-myc

    Expression

    Bacterial and Yeast

    Mutation

    Deleted the sec transport tag. Plasmid encodes H2…

    Promoter

    GAL1 and gal1

    Available since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETDuet RagA-RagC(D181N)

    Plasmid

    #99643

    Purpose

    Bacterial expression of RagA-RagC(D181N)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D181N

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge