We narrowed to 12,677 results for: sequence

1. pAluYa5-neo-TET

   Plasmid

   #51283

   Purpose

   expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   AluYa5
   

   Use

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Mutation

   Promoter

   upstream pol III enhancer sequence of the 7SL gen…

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. L4440_BioBrick-SCR-sgRNA-A

   Plasmid

   #177811

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-Sp-gRNA-AAVS1_B3903c_attP

   Plasmid

   #182144

   Purpose

   To insert attP attachment site at AAVS1 in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   AAVS1_B3903c_attP pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-Sp-gRNA-AAVS1_A3786c_attP

   Plasmid

   #182143

   Purpose

   To insert attP attachment site at AAVS1 in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   A3786c-attP pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-mCherry-CAAX

   Plasmid

   #129285

   Purpose

   Lentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence

   Depositor

   Carlos Carmona-Fontaine

   Article

   Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

   Insert

   Fluorescent reporter mCherry fused to CAAX polybasic sequence
   

   Use

   Lentiviral

   Tags

   CAAX

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PLG-bio-His

   Plasmid

   #53427

   Purpose

   Expresses full-length Plasminogen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PLG (PLG Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAG-flex-GFP-4x6T

   Plasmid

   #196418

   Purpose

   Cre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   GFP
   

   Use

   AAV and Cre/Lox; Astrocyte-selective

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. L4440_BioBrick-SCR-sgRNA-B

   Plasmid

   #177812

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57-GFP-2A-Cas9

   Plasmid

   #170805

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide

   Depositor

   Morten Frodin

   Article

   Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH_Str-KDEL_neomycin

    Plasmid

    #65307

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    

    Use

    Lentiviral

    Tags

    KDEL motif and Signal Sequence

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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