We narrowed to 3,800 results for: golden gate

1. pFC482

   Plasmid

   #246439

   Purpose

   The plasmid encodes a transcriptional unit for the expression of the CasE endoribonuclease fused with a nuclear localization signal

   Depositor

   Ron Weiss

   Article

   Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

   Insert

   CasE
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SapI domesticated

   Promoter

   EF-1a

   Available Since

   Nov. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEV824

   Plasmid

   #246453

   Purpose

   The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

   Depositor

   Ron Weiss

   Article

   Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

   Insert

   Gal4-VPR
   

   Use

   Synthetic Biology

   Mutation

   Mutated Kozak sequence

   Promoter

   miniYB

   Available Since

   Nov. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEV823

   Plasmid

   #246452

   Purpose

   The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

   Depositor

   Ron Weiss

   Article

   Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

   Insert

   Gal4-VPR
   

   Use

   Synthetic Biology

   Mutation

   Mutated Kozak sequence

   Promoter

   miniYB

   Available Since

   Nov. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEV746

   Plasmid

   #246449

   Purpose

   The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

   Depositor

   Ron Weiss

   Article

   Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

   Insert

   Gal4-VPR
   

   Use

   Synthetic Biology

   Mutation

   Mutated Kozak sequence

   Promoter

   miniYB

   Available Since

   Nov. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEV745

   Plasmid

   #246448

   Purpose

   The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

   Depositor

   Ron Weiss

   Article

   Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

   Insert

   Gal4-VPR
   

   Use

   Synthetic Biology

   Mutation

   Mutated Kozak sequence

   Promoter

   miniYB

   Available Since

   Oct. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEV785

   Plasmid

   #246451

   Purpose

   The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

   Depositor

   Ron Weiss

   Article

   Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

   Insert

   Gal4-VPR
   

   Use

   Synthetic Biology

   Mutation

   Mutated Kozak sequence

   Promoter

   miniYB

   Available Since

   Oct. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBA1729

   Plasmid

   #246063

   Purpose

   Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pTet promoter; p15a ori

   Depositor

   Jennifer Doudna

   Article

   Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

   Insert

   Panoptes locus
   

   Expression

   Bacterial

   Promoter

   pTet promoter

   Available Since

   Oct. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNP2314 [Cloning backbone, Gluc/Cluc Inversion Activity Reporter]

   Plasmid

   #247248

   Purpose

   Cloning backbone for measuring bridge recombinase mediated inversion

   Depositor

   Patrick Hsu

   Article

   Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTB105

   Plasmid

   #236778

   Purpose

   Backbone for cloning sgRNAs to be used with CRISPR Cas9 cytosine base editor. Expressed from the 18S rRNA SSU locus in Leishmania. Contains puromycin selection marker.

   Depositor

   Tom Beneke

   Article

   Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNP2885 [pNP2922 - ISCro4 TBL1, WT]

    Plasmid

    #247253

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA35-100 (TBL1)
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNP3413 [pNP2922 - ISCro4 TBL2 (WT) + ISCro4 Recombinase (WT)]

    Plasmid

    #247260

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA1-100 (TBL2)
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNP3396 [pNP2922 - ISCro4 DBL1 (WT) + ISCro4 Recombinase (WT)]

    Plasmid

    #247259

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA112-179 (DBL1)
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAG-mRuby2(miRE.FF4)-TS.FF6x1-bGH

    Plasmid

    #235297

    Purpose

    ComMAND open-loop circuit regulating mRuby2, CAG-driven

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG (synthetic cytomegalovirus early enhancer + c…

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a-mRuby2(miRE.FF4)-TS.FF4x4-bGH

    Plasmid

    #235295

    Purpose

    ComMAND closed-loop circuit regulating mRuby2 with 4 copies of microRNA target sites

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1a (human EF1a)

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKG3777_pGEEC584_pShip-EFS(promoter)-CMV(UTR)-mRuby2-bGH

    Plasmid

    #239746

    Purpose

    Plasmid expressing mRuby2 from the EFS canonical promoter with the CMV 5' UTR

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMKR07

    Plasmid

    #240097

    Purpose

    A CRISPR‑Cas9 system for knock‑out and knock‑in of high molecular weight DNA enables module‑swapping of the pikromycin synthase in its native host

    Depositor

    Adrian Keatinge-Clay

    Article

    Wang et al Res Sq [Preprint]. 2025 Mar 27:rs.3.rs-6

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    ermE* promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKG3666_pGEEC576_pShip-EFS-ZF43a(inducible)-P2A-iRFP720-bGH

    Plasmid

    #239740

    Purpose

    Plasmid expressing a rapamycin-inducible transcriptional activator

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKG3831_pGEEC579_pShip-UbC(no_intron)-mRuby2-bGH

    Plasmid

    #239742

    Purpose

    Plasmid expressing mRuby from a UbC promoter lacking an intron, used for modRNA production

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKG3774_pGEEC580_pShip-EF1a(no_intron)-mRuby2-bGH

    Plasmid

    #239743

    Purpose

    Plasmid expressing mRuby from an EF1a promoter lacking an intron, used for modRNA production

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKG3775_pGEEC582_pShip-hPGK(promoter)-CMV(UTR)-mRuby2-bGH

    Plasmid

    #239744

    Purpose

    Plasmid expressing mRuby2 from the hPGK canonical promoter with the CMV 5' UTR

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge