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Plasmid#161594PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XIII-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9344 (pgRNA_XVI-1_NatMX)
Plasmid#161596PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XVI-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
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gMUM2-Citrine
Plasmid#181965PurposeMUM2 protein fused to Citrine, under control of native promoterDepositorInsertMUM2 (MUM2 Mustard Weed)
UseTagsCitrine YFPExpressionPlantMutationPromoterMUM2Available sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
MUM2SP-Citrine
Plasmid#181963PurposeMUM2 signal peptide fused to Citrine, under control of MUM4_1.5kb promoterDepositorInsertMUM2 signal peptide, fused to the Citrine YFP in pAD
UseTagsCitrine YFPExpressionPlantMutationPromoterMUM4pro 1.5kb fragmentAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
L1-Ck1-hs1-l-chlo
Plasmid#163937PurposeFor integration in the chloroplast trnG_trnfM region: HS1-L, genome position: 40216_ 41843, Genbank accession no. MH635409DepositorInsertMarchantia polymorpha trnG_trnfM region left homologous sequence
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1-Ck4-hs1-r-chlo
Plasmid#163940PurposeFor integration in the chloroplast trnG_trnfM region: HS1-R, genome position: 41847_ 43622, Genbank accession no. MH635409DepositorInsertMarchantia polymorpha trnG_trnfM region right homologous sequence
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1-Ck4-hs2-r-chlo
Plasmid#163941PurposeFor integration in the chloroplast rbcL_trnR region: HS2-R, genome position: 57681- 59304, Genbank accession no. MH635409DepositorInsertMarchantia polymorpha rbcL_trnR region right homologous sequence
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PROM5_MpU6
Plasmid#136120PurposeMp U6 promoter (type III RNA polymerase promoter) to drive expression of gRNA for CRISPR/Cas9DepositorInsertPROM5_MpU6
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CDS_Cas9-NLS
Plasmid#136121PurposePuchta Arabidopsis codon-optimised Cas9 for CRISPRDepositorInsertCDS_AtcoCas9-NLS
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only