We narrowed to 22,780 results for: crispr

1. pU6-gRNA

   Plasmid

   #53062

   Purpose

   Transcription of guide RNA (gRNA) in wheat cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   wheat U6 promoter - gRNA
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   wheat U6

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-CDDR

   Plasmid

   #149355

   Purpose

   CRISPR/Cas9-based dual-fluorescent DSB repair reporter

   Depositor

   Tarek Abbas

   Article

   Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

   Inserts

   CDDR Reporter
   Blasticidin resistance
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hPGK

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti_sgRNA(MS2)_zsGreen1

   Plasmid

   #138463

   Purpose

   lenti sgRNA cloning backbone with MS2 loops and EF1a-zsGreen1

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   zsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQAT46

   Plasmid

   #223418

   Purpose

   T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQAT43

   Plasmid

   #223415

   Purpose

   T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-LbCas12a-D156R-ZmUbi-crRNA scaffold
   

   Use

   CRISPR; T-dna vector

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRE-KRAB-dCas9-IRES-GFP

   Plasmid

   #85556

   Purpose

   2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

   Depositor

   Eric Lander

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Insert

   KRAB-dCas9-IRES-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA Tag, and KRAB domain

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. CBX3 A6.5 gRNA

   Plasmid

   #90569

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CBX3

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CBX3 (Guide Designation A6.5)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CBX3 A5.5 gRNA

   Plasmid

   #90568

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CBX3

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CBX3 (Guide Designation A5.5)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQAT37

   Plasmid

   #223409

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQAT40

    Plasmid

    #223412

    Purpose

    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG Cas9-2A-Citrine

    Plasmid

    #92358

    Purpose

    CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    Cas9 2A Citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3xFLAG-dCas9/pCMV-7.1

    Plasmid

    #47948

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A (catalyticall…

    Promoter

    CMV

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330a dCas9-LSD1

    Plasmid

    #92362

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-LSD1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-MH6-EsCas13d

    Plasmid

    #108303

    Purpose

    Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Insert

    EsCas13d
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ131B2.0

    Plasmid

    #99885

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU3

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. G10 Cas9 MDC123

    Plasmid

    #59187

    Purpose

    G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection

    Depositor

    Robert Stupar

    Article

    Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

    Insert

    Glycine Max codon optimized Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    G10

    Available since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOSIP-KH-RBS2-dCas9

    Plasmid

    #182740

    Purpose

    Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

    Depositor

    David Bikard

    Article

    Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

    Insert

    dCas9
    

    Use

    CRISPR; Integrative vector

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-MH6-RspCas13d

    Plasmid

    #108305

    Purpose

    Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Insert

    RspCas13d
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. RRM1 E1.3 gRNA

    Plasmid

    #90880

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM1 (Guide Designation E1.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CASI-Cas9C-P2A-turboGFP

    Plasmid

    #80942

    Purpose

    Expresses Cas9C in mammalian cells; co-expresses turboGFP.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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