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We narrowed to 5,600 results for: Feng Zhang

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pHTN-HaloTag_SMCR8:1-937

Plasmid

#210984

Purpose

Mammalian expression of full-length human SMCR8. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

SMCR8:M1-I937 (SMCR8 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_SMCR8:1-937

Plasmid

#210985

Purpose

Mammalian expression of full-length human SMCR8. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

SMCR8:M1-I937 (SMCR8 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_FBXW5:1-566

Plasmid

#210981

Purpose

Mammalian expression of full-length human FBXW5. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW5:M1-R566 (FBXW5 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-C_PWP1:1-501

Plasmid

#210975

Purpose

Mammalian expression of full-length human PWP1. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

PWP1:M1-S501 (PWP1 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

E165D F448L G449R not annotated variants

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_MAGEA3:1-314

Plasmid

#210976

Purpose

Mammalian expression of full-length human MAGEA3. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

MAGEA3:M1-E314 (MAGEA3 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_MAGEA3:1-314

Plasmid

#210977

Purpose

Mammalian expression of full-length human MAGEA3. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

MAGEA3:M1-E314 (MAGEA3 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHTN-HaloTag_DCAF1:1-1507

Plasmid

#210972

Purpose

Mammalian expression of full-length human DCAF1. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF1:M1-E1507 (DCAF1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHTC-HaloTag_DCAF1:1-1507

Plasmid

#210973

Purpose

Mammalian expression of full-length human DCAF1. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF1:M1-E1507 (DCAF1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNLF1-C_RFWD3:1-774

Plasmid

#210955

Purpose

Mammalian expression of full-length human RFWD3. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RFWD3:M1-E774 (RFWD3 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_WDR55:1-383

Plasmid

#210957

Purpose

Mammalian expression of full-length human WDR55. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR55:M1-D383 (WDR55 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

C151R natural variant (VAR_037057)

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_DCAF15:1-600

Plasmid

#210958

Purpose

Mammalian expression of full-length human DCAF15. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF15:M1-L600 (DCAF15 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_WDR12:1-423

Plasmid

#210953

Purpose

Mammalian expression of full-length human WDR12. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR12:M1-A423 (WDR12 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WDR89:1-387

Plasmid

#211049

Purpose

Mammalian expression of full-length human WDR89. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR89:M1-Q387 (WDR89 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAX-1

Plasmid

#210013

Purpose

knock out BAX in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Apoptosis regulator BAX (BAX Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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PX458-sgTSC2

Plasmid

#128098

Purpose

CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

sgRNA against human TSC2 (TSC2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAX-2

Plasmid

#210130

Purpose

knock out BAX in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Apoptosis regulator BAX (BAX Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgBAK-2

Plasmid

#210132

Purpose

knock out BAK in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Bcl-2 homologous antagonist/killer (BAK1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgBAK-1

Plasmid

#210131

Purpose

knock out BAK in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Bcl-2 homologous antagonist/killer (BAK1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Cdk13_intron3_sg3_pX458

Plasmid

#127338

Purpose

sgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #3

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Mouse Cdk13 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

U6 Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Cdk13_intron4_sg4_pX330

Plasmid

#127341

Purpose

sgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #4

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Mouse Cdk13 Intron 4 Targeting sgRNA

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

U6 Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgABI2-1

Plasmid

#210139

Purpose

knock out ABI2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Abl interactor 2 (ABI2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgCYFIP1-1

Plasmid

#210137

Purpose

knock out CYFIP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgABI2-2

Plasmid

#210140

Purpose

knock out ABI2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Abl interactor 2 (ABI2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgHSPC300-1

Plasmid

#210141

Purpose

knock out HSPC300 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Protein BRICK1 (BRK1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgHSPC300-2

Plasmid

#210142

Purpose

knock out HSPC300 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Protein BRICK1 (BRK1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgCYFIP1-2

Plasmid

#210138

Purpose

knock out CYFIP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiSAMPHv2

Plasmid

#167934

Purpose

All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459_gRNA pAS dual_hspCas9

Plasmid

#193312

Purpose

Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

not applicable

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330 spCas9-mSA

Plasmid

#113096

Purpose

pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

CAS9-MSA

Use

Synthetic Biology

Tags

MSA (monomeric streptavidin

Expression

Mutation

none

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LES2A-CRE

Plasmid

#167933

Purpose

Lentiviral CRE expression tracked by mScarlet-I fluorescence marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Insert

mScarlet-P2A-CRE

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge