We narrowed to 3,813 results for: biorxiv

1. pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

   Plasmid

   #220494

   Purpose

   To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

   Depositor

   Sébastien Britton

   Article

   Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

   Insert

   sgRNA targeting LIG4 exon 3 (LIG4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

   Plasmid

   #195018

   Purpose

   An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

   Depositor

   Christopher Richie

   Article

   Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

   Inserts

   CCGTATAGGCGGCTGCCCAA
   EGFP-KASH
   TTCCGTGGTGGGCAACGTAG
   

   Use

   AAV

   Tags

   KASH domain of Nesprin2

   Expression

   Mutation

   Promoter

   EF1a, hU6, and mU6

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-CAG-flex-iGABASnFR2n-WPRE

   Plasmid

   #218881

   Purpose

   AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2n
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   S99A F104H R168P

   Promoter

   CAG

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE

   Plasmid

   #218873

   Purpose

   AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Has Service

   AAV1 and AAV5

   Insert

   iGABASnFR2(no bind)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S99A F102G R168P

   Promoter

   GFAP

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC1_oROS-HT_LF(C199S)

   Plasmid

   #216412

   Purpose

   Expresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT_LF(C199S)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC1-lifeact-oROS-HT

   Plasmid

   #216420

   Purpose

   Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   lifeact-oROS-HT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pC3.1_CMV_oROS-G_LF(C199S)

   Plasmid

   #216112

   Purpose

   Expresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.01.31.578117

   Insert

   oROS-G_LF(C199S)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pToxEXPr6

   Plasmid

   #218290

   Purpose

   Introduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   hph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJQR3

   Plasmid

   #218289

   Purpose

   Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   HO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036W

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pITEdv1

    Plasmid

    #218283

    Purpose

    Use together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locus

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    Hph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pITEu2

    Plasmid

    #218282

    Purpose

    Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJE13HD7

    Plasmid

    #218281

    Purpose

    Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mouse b6 full length

    Plasmid

    #217816

    Purpose

    Full length mouse integrin b6 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGB6 (Itgb6 Mouse)
    

    Use

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. eGFP-SUN1ΔN

    Plasmid

    #213508

    Purpose

    Expresses human eGFP-SUNΔN in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    eGFP-SUNΔN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SUN1ΔN has the first 138 amino acid (lamina domai…

    Promoter

    CMV

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KU70-eGFP-SUN1ΔN

    Plasmid

    #213516

    Purpose

    Expresses human KU70-eGFP-SUN1ΔN in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    KU70-eGFP-SUNΔN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SUN1ΔN has the first 138 amino acid (lamina domai…

    Promoter

    CMV

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGP-AAV-syn-iGABASnFR2(no bind)-WPRE

    Plasmid

    #218876

    Purpose

    AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Has Service

    AAV1 and AAV5

    Insert

    iGABASnFR2(no bind)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102G R168P

    Promoter

    Synapsin

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV2_CAG_oROS-G_LF(C199S)

    Plasmid

    #216116

    Purpose

    Encodes the loss-of-function mutation C199S of the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G_LF(C199S)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)

    Plasmid

    #208869

    Purpose

    For mammalian expression of NAP1 (delta-TBK1)

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L226Q/L233Q (disrupts TBK1 binding)

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)

    Plasmid

    #208872

    Purpose

    For expression in E. coli of NAP1 (delta-NDP52)

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    S37K/A44E (disrupts NDP52 binding)

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)

    Plasmid

    #208873

    Purpose

    For expression in E. coli of NAP1 (delta-TBK1)

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    L226Q/L233Q (disrupts TBK1 binding)

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge