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XZ024 CMV-TO-bArrestin2-MCP (FLP-IN)

Plasmid

#233379

Purpose

ARRB2-tdMCP with a short linker

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ARRB2-tdMCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Plasmid

#125231

Purpose

For generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirus

Depositor

Alice Ting

Publication

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

Plasmid

#125235

Purpose

Expresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cells

Depositor

Alice Ting

Publication

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRESTA-FLAG-CLIP-2XTPR-SNAP-StrepTag

Plasmid

#248183

Purpose

For the bacterial expression of FLAG-CLIP-2XTPR-SNAP-StrepTag fusion protein.

Depositor

Alison Twelvetrees

Publication

Smith et al Adv Sci (Weinh). 2026 Jul 21:e76672. doi

Insert

FLAG-CLIP-2XTPR-SNAP-StrepTag

Use

1476

Tags

HisTag, FLAG, CLIP and SNAP, StrepTag

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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Nb24-His6 (b-arrestin1 nanobody)

Plasmid

#259692

Purpose

E. coli periplasmic expression of the active-state-specific beta-arrestin1 (arrestin2) nanobody Nb24 with a C-terminal His6 tag

Depositor

Laura Wingler

Publication

Elgeti et al J Biol Chem. 2026 Feb;302(2):111117. doi

Insert

Nb24-His6

Tags

His6

Expression

Bacterial

Promoter

plac

Available since

Aug. 17, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LATeNT(slow)-Arrestin

Plasmid

#240984

Purpose

Expresses LATeNT*-Arrestin in mammalian cells; used with CCR6-NanoLuc or KORD-NanoLuc

Depositor

Alice Ting

Publication

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

LATeNT*-myc-Arrestin

Tags

myc (internal)

Expression

Mammalian

Available since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET His6 TEV cloning vector with BioBrick polycistronic restriction sites (9B)

Plasmid

#48284

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6

Expression

Bacterial

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)

Plasmid

#48295

Depositor

Scott Gradia

Publication

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6

Expression

Insect

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET His6 Sumo TEV expression vector with BioBrick polypromoter restriction sites (14-S)

Plasmid

#48313

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and Sumo

Expression

Bacterial

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRESTA-FLAG-CLIP-1XTPR-SNAP-StrepTag

Plasmid

#248182

Purpose

For the bacterial expression of FLAG-CLIP-1XTPR-SNAP-StrepTag fusion protein.

Depositor

Alison Twelvetrees

Publication

Smith et al Adv Sci (Weinh). 2026 Jul 21:e76672. doi

Insert

FLAG-CLIP-1XTPR-SNAP-StrepTag

Use

1374

Tags

HisTag, FLAG, CLIP and SNAP, StrepTag

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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pRESTA-FLAG-CLIP-3XTPR-SNAP-StrepTag

Plasmid

#248184

Purpose

For the bacterial expression of FLAG-CLIP-3XTPR-SNAP-StrepTag fusion protein.

Depositor

Alison Twelvetrees

Publication

Smith et al Adv Sci (Weinh). 2026 Jul 21:e76672. doi

Insert

FLAG-CLIP-3XTPR-SNAP-StrepTag

Use

1578

Tags

HisTag, FLAG, CLIP and SNAP, StrepTag

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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pET MBP TEV expression vector with BrioBrick polypromoter restriction sites (14-Q)

Plasmid

#48311

Depositor

Scott Gradia

Type

Empty backbone

Tags

MBP

Expression

Bacterial

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGS-M1-BRG1-3xFLAG-BAF57

Plasmid

#109189

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged BRG1 and untagged BAF57 under the control of a p10 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Publication

Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-

Inserts

BRG1 (SMARCA4 Human)
BAF57 (SMARCE1 Human)

Tags

3xFLAG

Expression

Insect

Promoter

p10

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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APL0030 SFFV-bArrestin2-MCP (FLP-IN)

Plasmid

#233436

Purpose

SFFV driven ARRB2-tdMCP with a short linker

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ARRB2-tdMCP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-Crestin-pro (JDW 1320)

Plasmid

#229830

Purpose

A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Crestin promoter, c-fos minimal promoter, and b-globin intron

Use

Gateway Cloning

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3 CMV myc-Arrestin-TEVp

Plasmid

#173115

Purpose

Expresses arrestin receiver construct in mammalian cells

Depositor

Alice Ting

Publication

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

myc-Arrestin-TEVp

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-Restored-Hairpin

Plasmid

#136033

Purpose

EIF3B (465-552) 3'UTR with the main hairpin restored inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Publication

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET StrepII TEV expression vector with BioBrick polypromoter restriction sites (14-R)

Plasmid

#48312

Depositor

Scott Gradia

Type

Empty backbone

Tags

StrepII

Expression

Bacterial

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 Thioredoxin TEV cloning vector with BioBrick polycistronic restriction sites (9T)

Plasmid

#48292

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and Thioredoxin

Expression

Bacterial

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 Sumo TEV cloning vector with BioBrick polycistronic restriction sites (9S)

Plasmid

#48291

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and Sumo

Expression

Bacterial

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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