We narrowed to 3,806 results for: golden gate

1. EF1a-TS.FF4x1-mRuby2-bGH

   Plasmid

   #235274

   Purpose

   3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1a (human EF1a)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EF1a-TS.FF6x1-mRuby2(miRE.FF4)-bGH

   Plasmid

   #235288

   Purpose

   ComMAND open-loop circuit regulating mRuby2 with miRE-FF4 design 2

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1a (human EF1a)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EF1a-TS.FF6x1-mRuby2-miRE.FF4-bGH

   Plasmid

   #235290

   Purpose

   ComMAND open-loop circuit regulating mRuby2 with miRE-FF4 design 3

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1a (human EF1a)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EFS-mRuby2(miRE.FF4)-TS.FF4x1-bGH

   Plasmid

   #235310

   Purpose

   ComMAND closed-loop circuit regulating mRuby2, EFS-driven

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EFS (human EF1a short, intronless)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EF1a-TS.FF4x1-mRuby2-miR.FF4-bGH

   Plasmid

   #235287

   Purpose

   ComMAND closed-loop circuit regulating mRuby2 with miR-FF4 design 3

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1a (human EF1a)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EF1a-TS.FF4x1-mRuby2-miRE.FF4-bGH

   Plasmid

   #235291

   Purpose

   ComMAND closed-loop circuit regulating mRuby2 with miRE-FF4 design 3

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1a (human EF1a)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EF1a-TS.FF5x1-mRuby2(miRE.FF5)-bGH

   Plasmid

   #235263

   Purpose

   ComMAND closed-loop circuit regulating mRuby2 with miRE-FF5 design 2

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1a (human EF1a)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EF1a-TS.FF6x1-mRuby2-bGH

   Plasmid

   #235275

   Purpose

   3' microRNA target site expression for dual-vector iFFL circuit (open-loop)

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1a (human EF1a)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EF1a-TS.FF6x1-mRuby2-miR.FF4-bGH

   Plasmid

   #235286

   Purpose

   ComMAND open-loop circuit regulating mRuby2 with miR-FF4 design 3

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1a (human EF1a)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EF1a-mRuby2-TS.FF4x1-bGH

    Plasmid

    #235272

    Purpose

    3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EF1a-mRuby2-TS.FF6x1-bGH

    Plasmid

    #235273

    Purpose

    3' microRNA target site expression for dual-vector iFFL circuit (open-loop)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EF1a-mRuby2(miRE.FF4)-TS.FF6x2-bGH

    Plasmid

    #235292

    Purpose

    ComMAND open-loop circuit regulating mRuby2 with 2 copies of microRNA target sites

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pM-mCardinal

    Plasmid

    #228630

    Purpose

    Mammalian expression of mCardinal and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mCardinal
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pM-mPapaya

    Plasmid

    #228626

    Purpose

    Mammalian expression of mPapaya and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mPapaya
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAPH07

    Plasmid

    #234345

    Purpose

    Library-scale IPTG-inducible gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK

    Depositor

    Jerome Fox

    Article

    Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12

    Insert

    Type II dCas9 sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAPH021

    Plasmid

    #234346

    Purpose

    Library-scale IPTG-inducible single transcript dual-gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK

    Depositor

    Jerome Fox

    Article

    Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12

    Inserts

    Type II dCas9 sgRNA
    Type II dCas9 sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Reckleen_1_Cas9

    Plasmid

    #233456

    Purpose

    Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

    Depositor

    Anke Becker

    Article

    Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

    Insert

    Level 2 RECKLEEN plasmid
    

    Use

    CRISPR

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHW1387

    Plasmid

    #229880

    Purpose

    Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    

    Tags

    GFP-C1

    Expression

    Worm

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHW1223

    Plasmid

    #229875

    Purpose

    gene trap RMCE plasmid with LexA[LexA(DBD)-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    LexA[LexA(DBD)-linker-QFAD]-act-4 3'UTR::SEC
    

    Expression

    Worm

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHW1222

    Plasmid

    #229874

    Purpose

    gene trap RMCE plasmid with QF2[QF(DBD)-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    QF2[QF(DBD)-linker-QFAD]-act-4 3'UTR::SEC
    

    Expression

    Worm

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge