We narrowed to 15,479 results for: pET

Showing: 3201 - 3220 of 15479 results

Results per page:

Sort results by:

pETDuet RagA-RagC(D181N)

Plasmid

#99643

Purpose

Bacterial expression of RagA-RagC(D181N)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

D181N

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b-mEB3-mCherry-6xHis

Plasmid

#130985

Purpose

Bacterial expression of mouse EB3-mCherry-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB3-mCherry-6xHis (Mapre3 Mouse)

Tags

mCherry-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-LanCL2 (6xHis-LanCL2)

Plasmid

#73407

Purpose

Expresses His tagged LanCL2

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

LanCL2 (LANCL2 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
pET21a(+)-Histag-Cerulean (526)

Plasmid

#70716

Purpose

Expression vector for Cerulean purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Cerulean

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-his6-HA3-HALO

Plasmid

#188943

Purpose

T7 expression vector for making his6- and HA3-tagged HALO protein

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-HALO

Expression

Bacterial

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22-His-TEV-DOT1L-MM

Plasmid

#89611

Purpose

Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

Depositor

Leemor Joshua-Tor

Article

Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

Insert

Dot1L VVEL293MM Mutant (DOT1L Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-StrepII-TagRFP-CohI

Plasmid

#58710

Purpose

Detection construct to label dockerin type I containing fusion proteins

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

TagRFP-CohI

Tags

StrepII

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Pet15b-Endosomal Escape Control

Plasmid

#186552

Purpose

Negative Control confirming the need for a CPP peptide in mediating internalization

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

6H-ETA-GFP

Tags

6xHis and EGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Pet15b-Internalization and Endosomal Escape

Plasmid

#186551

Purpose

Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

10R-8His-ETA-EGFP

Tags

8xHis and EGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM10 with rat FL Annexin A11

Plasmid

#164496

Purpose

For bacterial expression of rat His-tagged FL Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat FL Annexin A11 (Anxa11 Rat)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-FLAG-SpCas9-HF1

Plasmid

#126770

Purpose

Expression of increased fidelity SpCas9-HF1 in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+) 6xHis-TEV-IFIT3

Plasmid

#53559

Purpose

bacterial expression of IFIT3

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT3 (IFIT3 Human)

Tags

6xHIS and TEV

Expression

Bacterial

Promoter

T7

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pET151-D-TOPO-PARP14-SΔK

Plasmid

#216658

Purpose

Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

Depositor

Herwig Schüler

Article

Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

Insert

PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

Deletion starting from Q1390 to D1448 and substit…

Promoter

T7

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pETconNK-TEM1(S70A,D179G)

Plasmid

#81166

Purpose

pETconNK with TEM1(S70A,D179G) beta-lactamase

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

TEM-1 beta-lactamase

Tags

c-myc

Expression

Bacterial and Yeast

Mutation

Deleted the sec transport tag. Plasmid encodes H2…

Promoter

GAL1 and gal1

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a-Med25-VBD-6His

Plasmid

#64773

Purpose

Bacterial expression of His-tagged Med25-VBD

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25-VBD (MED25 Human)

Expression

Bacterial

Mutation

insert is 394-543 only

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pET22b 6His SpyCatcher-GRFT

Plasmid

#204502

Purpose

Bacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linker

Depositor

Urartu Seker

Article

Ozkul et al Adv Mater Interfaces. 2022 Sep 13:220112

Insert

GRFT

Use

Synthetic Biology

Tags

6xHis and SpyCatcher

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-AMPKsub-YPet-NES

Plasmid

#84635

Purpose

Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

AMPKsub-YPet-NES

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_DmKHC[1-421]-SNAP-6xHis

Plasmid

#196975

Purpose

Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

Depositor

Lukas Kapitein

Article

Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

Insert

Kinesin Heavy Chain (Khc Fly)

Tags

6xHis and SNAP

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-deSpCas9-VP64-6xHis

Plasmid

#92117

Purpose

Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive eSpCas9-NLS-3xFLAG-VP64

Use

CRISPR

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A, H840A, K848A, K1003A, R1060A

Promoter

T7

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-DEST42-ARF6-V5-His6

Plasmid

#67415

Purpose

Expression of hArf6 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only