We narrowed to 15,088 results for: pET

1. pET42b-A3A-PBE-ΔUGI

   Plasmid

   #119771

   Purpose

   To express the recombinant APOBEC3A in vitro

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-NLS
   

   Tags

   6*HIS and NLS

   Expression

   Bacterial

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   T7 promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDuet RagA-RagC(S75N)

   Plasmid

   #99645

   Purpose

   Bacterial expression of RagA-RagC(S75N)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   S75N

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-22HT-MSI2(8-193)

   Plasmid

   #60356

   Purpose

   Express the RBD of human MSI2 in bacterial cells for protein purification

   Depositor

   Sean Ryder

   Article

   Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

   Insert

   human MSI2 (8-193) (MSI2 Human)
   

   Tags

   His and TEV protease site

   Expression

   Bacterial

   Mutation

   contains amino acids 8-193 of MSI2

   Promoter

   T7

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM6-Pc4CL-CmCHS-MsCHI

   Plasmid

   #73398

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   Pc4CL
   CmCHS
   MsCHI
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. WT N-His Ana GvpC in pET28a

   Plasmid

   #153294

   Purpose

   Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   Gas vesicle protein C (N-terminal his-tagged)
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Added a hexahistidine tag after the methionine at…

   Promoter

   T7

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pet16b ALIX Bro1 (1-359)

   Plasmid

   #80641

   Purpose

   Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428

   Depositor

   Wesley Sundquist

   Article

   McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

   Insert

   ALIX Bro1, residues 1-359 (PDCD6IP Human)
   

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Residues 1-359

   Promoter

   T7

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pet151D-TOPO BROX 1-411;

   Plasmid

   #89602

   Purpose

   Expresses full length human Brox in bacteria; internal ID WISP11-297

   Depositor

   Wesley Sundquist

   Article

   Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

   Insert

   BROX (BROX Human)
   

   Tags

   His Tag, V5 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pF714-pET28a-Hs-FL-METTL20-NHis wt

   Plasmid

   #85115

   Purpose

   expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human full-length METTL20 (METTL20 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pET51b-Strep-hAGT-His

   Plasmid

   #167276

   Purpose

   Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   hAGT (AGT Human)
   

   Tags

   His x10 and Strep-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-sfGFP[D134TAG+N150TAA]

    Plasmid

    #164582

    Purpose

    superfolder GFP with D134TAG and N150TAA mutations

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Insert

    sfGFP-134TAG+150TAA
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D134TAG + N150TAA Mutations

    Promoter

    T7

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET 15b-UbcH5c/2418

    Plasmid

    #11473

    Depositor

    Allan Weissman

    Article

    Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

    Insert

    UbcH5c (UBE2D3 Human)
    

    Expression

    Bacterial

    Mutation

    n/a

    Available Since

    March 8, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pET28a(+) 6xHis-TEV-IFIT5

    Plasmid

    #53560

    Purpose

    bacterial expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET-30a(+)-Syk-tSH2

    Plasmid

    #111269

    Purpose

    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-Hisx6-Sirt6-H133Y

    Plasmid

    #20279

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Histidine 133 to Tyrosine

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pET151-Vps4(101-437)

    Plasmid

    #87735

    Purpose

    Bacterial expression of N-terminally truncated S. cerevisiae Vps4

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETNKI-strepII-3C-LIC-kan

    Plasmid

    #108706

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-MBP-CfaN-His6

    Plasmid

    #126017

    Purpose

    Bacterial expression of MBP-CfaN fusion protein (with C-terminal His5)

    Depositor

    Thomas Muir

    Insert

    MBP-CfaN-His5
    

    Tags

    5xHis

    Expression

    Bacterial

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET11a_3xFlag-nsp10 (SARS-CoV-2)

    Plasmid

    #169157

    Purpose

    To express SARS-CoV-2 nsp10 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-nsp5CS[VRLQ]-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    3xFlag and nsp5CS[VRLQ]: predicted nsp5 protease …

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM6-At4CL-PhCHS-CmCHI

    Plasmid

    #73393

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    PhCHS
    CmCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET11a-CA(A14C, E45C, A92E)

    Plasmid

    #79046

    Purpose

    Bacterial vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    CA (A14C, E45C, A92E)
    

    Expression

    Bacterial

    Mutation

    A14C, E45C, A92E mutations in HIV-1 CA protein

    Promoter

    T7

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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