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We narrowed to 11,030 results for: Kars;

Showing: 6481 - 6520 of 11030 results

  6. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23

    Plasmid
    #111723
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q23 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111723-plasmid-map-sequence-id-220473

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  11. pDONR223 SARS-CoV-2 NSP5

    Plasmid
    #141259
    Purpose
    Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available Since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141259-plasmid-map-sequence-id-288065

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  13. TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

    Plasmid
    #216155
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216155-plasmid-map-sequence-id-437763

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  15. pAB1677 REPAIR.t3

    Plasmid
    #176320
    Purpose
    CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt3 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176320-plasmid-map-sequence-id-345287

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  24. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q79

    Plasmid
    #111729
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q79 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111729-plasmid-map-sequence-id-226812

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  26. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q19

    Plasmid
    #111741
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q19 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111741-plasmid-map-sequence-id-220475

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  28. TRIPZ-CTID195-GSQ-UBnc-PURO

    Plasmid
    #208037
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    Ubnc (UBC Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Expression
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208037-plasmid-map-sequence-id-410268

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  30. pAB1923 REPAIR.t1-S

    Plasmid
    #176321
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q/E620G/Q696L, only the deaminase domain (aa …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176321-plasmid-map-sequence-id-345288

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  31. CIRTS-FTO-control

    Plasmid
    #216859
    Purpose
    CIRTS RNA targeting system for targeted removal of m6A on transcript at the synapse. CIRTS-FTO-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100
    Insert
    CIRTS-FTO-Calm3-U6-control gRNA
    Use
    Lentiviral
    Tags
    mCherry2
    Expression
    Mammalian
    Mutation
    Promoter
    Syn1, U6
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216859-plasmid-map-sequence-id-430696

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  39. pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

    Plasmid
    #105127
    Purpose
    Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein
    Depositor
    Kimberley Evason, Didier Stainier
    Article
    Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. d
    Inserts
    Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
    Use
    Zebrafish transgenesis
    Tags
    Expression
    Mutation
    Promoter
    cryaa (crystallin) and fabp10a
    Available Since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    105127-plasmid-map-sequence-id-220518

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Showing: 6481 - 6520 of 11030 results