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167_pAAV-ProC34-CatCh-GFP-WPRE

Plasmid

#125968

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC34

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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13_pAAV-ProC71-CatCh-GFP-WPRE

Plasmid

#125976

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC71

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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159_pAAV-ProD3-CatCh-GFP-WPRE

Plasmid

#125979

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProD3

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyanpsin.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209663

Purpose

Expression of iATPSnFR2, high affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Mutation

S29W.A95K

Promoter

hSyn

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209659

Purpose

Expression of iATPSnFR2, high affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Mutation

S29W.A95K

Promoter

GFAP

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209657

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

CAG

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-PiGM-Ii (149, 535/81, eGFP)

Plasmid

#221610

Purpose

AAV encoding the 149 amino acid version PiGM-Ii with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).

Depositor

John Lin

Insert

PiGM-Ii (149, eGFP)

Use

AAV

Mutation

RGS10(truncation after amino acid 149)

Promoter

Human Synapsin

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

Plasmid

#113042

Purpose

AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

Plasmid

#113041

Purpose

AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-PGD2-1.1

Plasmid

#214764

Purpose

AAV packaging plasmid for GRAB PGD2-1.1 fluorescent sensor under EF1alpha promoter

Depositor

Yulong Li

Article

Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

Insert

GRAB-PGD2-1.1 sensor

Use

AAV

Promoter

EF-1-alpha promoter

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40

Plasmid

#194688

Purpose

hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2603_AAV-Nme2-ABE8e-i1-U6-Rosa26

Plasmid

#201685

Purpose

All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 driven sgRNA targeting the mouse Rosa26 gene

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U1a

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Pcdh15_mini_V7-CD2

Plasmid

#199186

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V7-CD2

Use

AAV

Expression

Mammalian

Mutation

EC4, EC5, EC6, EC9, EC10 are deleted from msPCDH1…

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Pcdh15_mini_V8-CD2

Plasmid

#199187

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V8-CD2

Use

AAV

Mutation

EC5, EC6, EC8, EC9, EC10 are deleted from msPCDH1…

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL

Plasmid

#172195

Purpose

AAV sequence-specific control vector for TALE-Tet1FL-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

Depositor

Garrett Kaas

Article

Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

Insert

HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL

Use

AAV and Mouse Targeting; Tale

Tags

HA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

transcription regulatory domain removed

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α1.1-SomArchon-GFP

Plasmid

#153531

Purpose

AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter.

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-GFP

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

EF1α1.1

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-APLP1ICD-IRES-hrGFP

Plasmid

#107546

Purpose

AAV-mediated expression of last 50 amino acids of APLP1 protein (APLP1ICD) and IRES-mediated co-expression of hrGFP to recognize labelled cells

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

APLP1ICD (APLP1 Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-GCaMP6f-WPRE

Plasmid

#141237

Purpose

AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

GCaMP6f

Use

AAV and Cre/Lox

Tags

6XHIS and Xpress

Promoter

Human elongation factor-1 alpha (EF-1 alpha)

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1617 pAAV SYN1 Nuc-EYFP

Plasmid

#135567

Purpose

An AAV vector expressing a neuronally expressed nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

empty
Nuc-EYFP

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

hSYN1

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-LSL-mCherry-shLuci

Plasmid

#178590

Purpose

Cre-dependent expression of mCherry and a shRNA against luciferase under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

miRE-Luciferase

Use

AAV

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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