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RecV recombinase system for in vivo targeted optogenomic modifications of single cells or cell populations.
Yao S, Yuan P, Ouellette B, Zhou T, Mortrud M, Balaram P, Chatterjee S, Wang Y, Daigle TL, Tasic B, Kuang X, Gong H, Luo Q, Zeng S, Curtright A, Dhaka A, Kahan A, Gradinaru V, Chrapkiewicz R, Schnitzer M, Zeng H, Cetin A
Nat Methods. 2020 Mar 23. pii: 10.1038/s41592-020-0774-3. doi: 10.1038/s41592-020-0774-3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
140131pAAV-EF1a-NCreVcan be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer
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140132pAAV-EF1a-CCreVcan be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer
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140133pAAV-EF1a-CDreVcan be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer
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140135pAAV-EF1a-iCreVcan be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase
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140136pAAV-EF1a-iDreVcan be used to generate AAV virus that will expresslight-inducible site-specific iDreV recombinase
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140137pAAV-EF1a-iFlpVcan be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase
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