We narrowed to 3,903 results for: biorxiv

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pLenti-rTetR(SE)-VP64-2A-mCherryBSD

Plasmid

#225695

Purpose

Lentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Publication

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-VP64

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mCherry

Expression

Mammalian

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-EED-2A-mTurquoise

Plasmid

#225692

Purpose

Lentiviral expression of rTetR(SE) fused to EED and mTurquoise-NLS

Depositor

Lacra Bintu

Publication

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-EED (EED Human, Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-HP1a-2A-mTurquoise

Plasmid

#225691

Purpose

Lentiviral expression of rTetR(SE) fused to HP1a and mTurquoise-NLS

Depositor

Lacra Bintu

Publication

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

HP1a (CBX5 Human, Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-KRAB-2A-mTurquoise

Plasmid

#225690

Purpose

Lentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLS

Depositor

Lacra Bintu

Publication

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-ZNF10 KRAB

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGLU_85

Plasmid

#225800

Purpose

This is the promoter region taken from upstream of Himar transposase in pMarC9-Kan. It can be considered the "default" promoter to use.

Depositor

Nili Ostrov

Publication

Gilbert et al bioRxiv 2025.12.22.696024

Insert

PmarC9_[Himar]

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuideB3GNT5-neo

Plasmid

#220867

Purpose

encodes CRISPR sgRNA targeting human B3GNT5

Depositor

Jennifer Kohler

Publication

Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0

Insert

B3GNT5 (B3GNT5 Human)

Use

Lentiviral

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuideSLC35C1-neo

Plasmid

#220868

Purpose

encodes CRISPR sgRNA targeting human SLC3C1

Depositor

Jennifer Kohler

Publication

Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0

Insert

SLC35C1 (SLC35C1 Human)

Use

Lentiviral

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Plasmid

#222990

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Publication

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Promoter

tac

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Plasmid

#222987

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Publication

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

Plasmid

#220494

Purpose

To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

Depositor

Sébastien Britton

Publication

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

sgRNA targeting LIG4 exon 3 (LIG4 Human)

Use

CRISPR

Promoter

U6

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mc2 hygro hPGK

Plasmid

#206228

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Publication

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 puro hPGK

Plasmid

#206227

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Publication

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 neo hPGK

Plasmid

#206226

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Publication

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 hygro EF1a

Plasmid

#206222

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Publication

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 hygro

Plasmid

#206216

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Publication

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_144

Plasmid

#199091

Purpose

Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_141

Plasmid

#199088

Purpose

Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_175

Plasmid

#199107

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

Dummy ORI plus NGS barcode 23, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_172

Plasmid

#199104

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSG5 ORI plus NGS barcode 20, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_145

Plasmid

#199092

Purpose

Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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