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pTn7-SCOUT11.G

Plasmid

#200999

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hArfaptin2

Plasmid

#206405

Purpose

Mammalian expression of hArfaptin2

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Publication

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin2 (ARFIP2 Human)

Tags

EGFP

Expression

Mammalian

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP-ATG13(315)

Plasmid

#122072

Purpose

Expressing GFP-Atg13

Depositor

Daniel Klionsky

Publication

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

GFP

Expression

Yeast

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pNB2176

Plasmid

#113395

Purpose

Ty3-IN-GFP; alpha factor inducible

Depositor

Suzanne Sandmeyer

Publication

Beliakova-Bethell et al RNA. 2006 Jan;12(1):94-101. doi: 10.1261

Insert

Ty3 with integrase-green fluorescent protein

Tags

IN-GFP

Expression

Yeast

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-GGA2-msGFPx3

Plasmid

#105258

Purpose

Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-msGFPx3.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Gga2 portion (GGA2 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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Reb_79 aka PAS620

Plasmid

#71225

Purpose

Reb_1 with RebA(Q91R)

Depositor

Pamela Silver

Publication

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA( G53A, Q91R)

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
Reb_80 aka PAS621

Plasmid

#71226

Purpose

Reb_1 with RebB(I67T, N84I)

Depositor

Pamela Silver

Publication

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebB(I67T, N84I)

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
Reb_65 aka PAS626

Plasmid

#71231

Purpose

Reb_1 with RebA(Y82A)

Depositor

Pamela Silver

Publication

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA(G53A, Y82A)

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
SNAP-EF(N)-60nmSAH-GFP(1-10)

Plasmid

#61663

Purpose

2x-30nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Publication

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNAP-EF(N)-60nmSAH-GFP(1-10)

Expression

Mammalian

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pg50-2.11 (#65)

Plasmid

#35966

Depositor

Thomas Sato

Publication

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Insert

Tie2 promoter-1st exon-1st intron

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pTYB2-PAB1

Plasmid

#37234

Depositor

Jon Lorsch

Publication

Park et al EMBO J. 2011 Jan 19;30(2):302-16. Epub 2

Insert

PAB1 (PAB1 Budding Yeast)

Tags

intein

Expression

Bacterial

Promoter

T7

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pSicoR-mCh-Chd1i4

Plasmid

#21908

Depositor

Miguel Ramalho-Santos

Publication

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Insert

Chd1-i4

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF5

Plasmid

#13104

Depositor

Keith Joung

Publication

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF5

Expression

Mammalian

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF63

Plasmid

#13199

Depositor

Daniel Voytas

Publication

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF63

Expression

Mammalian

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMS23: pHelper(PmcCAST)_entry_ΔTnsD

Plasmid

#168156

Purpose

Inducible expression of PmcCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Publication

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ

Expression

Bacterial

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046457

Plasmid

#145424

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGEMHE-Pom121-mScarlet

Plasmid

#171491

Purpose

T7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA

Depositor

Melina Schuh

Publication

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

Pom121 (Pom121 Mouse)

Use

Pgehme

Tags

mScarlet

Promoter

T7

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-PIK1

Plasmid

#105260

Purpose

Plasmid for pop-in/pop-out replacement of Pik1 with iGFP-Pik1.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Pik1 (PIK1 Budding Yeast)

Tags

iGFP

Expression

Yeast

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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TAL2065

Plasmid

#39435

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-39-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL2064

Plasmid

#39434

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-39-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only