We narrowed to 11,646 results for: emb

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TAL2065

Plasmid

#39435

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-39-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL2064

Plasmid

#39434

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-39-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA1-Tastin

Plasmid

#11871

Depositor

Michiko Fukuda

Publication

Fukuda et al Genes Dev. 1995 May 15. 9(10):1199-210.

Insert

Tastin (TROAP Human)

Expression

Mammalian

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pAAV hSyn INTRON mScarlet-CAAX2 WPRE

Plasmid

#236232

Purpose

AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Publication

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mScarletI

Promoter

human Synapsin 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pYes2-AtPCS1

Plasmid

#49766

Purpose

Expresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Publication

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

AtPCS1 (PCS1 Mustard Weed)

Expression

Yeast

Promoter

GAL1

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pDEST14-DoubleCatcher

Plasmid

#216284

Purpose

Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders

Depositor

Mark Howarth

Publication

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

DoubleCatcher

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AXBE

Plasmid

#204605

Purpose

Mammalian expression of adenine transversion base editor AXBE

Depositor

Dali Li

Publication

Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

Insert

bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

S. pyogenes Cas9 (D10A)

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGEMHE-H2B-mClover3

Plasmid

#171489

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged human H2B mRNA

Depositor

Melina Schuh

Publication

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

H2B (H2BC11 Human)

Use

Pgehme

Tags

mClover3

Promoter

T7

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSUPER-ARF1b

Plasmid

#67494

Purpose

Expression of shRNA targeting human ARF1b

Depositor

Richard Kahn

Publication

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF1 shRNA (ARF1 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pTAL4

Plasmid

#31035

Depositor

Adam Bogdanove, Daniel Voytas

Publication

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

TALEN-FokI nuclease backbone

Tags

AcV5 and FokI nuclease

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pCI-OR51L1

Plasmid

#22325

Depositor

Hiroaki Matsunami

Publication

Saito et al Sci Signal. 2009 Mar 3;2(60):ra9. doi: 1

Insert

OR51L1 (OR51L1 Human)

Tags

Rho

Expression

Mammalian

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pCC1-4k-BBclone2

Plasmid

#137070

Purpose

E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

Depositor

Debra Hansen

Publication

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Inserts

BB0323
P13
DipA
Lmp1

Expression

Bacterial

Mutation

wild-type, full-length (signal-peptide-containing…

Available since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1

Plasmid

#224773

Purpose

Mammalian expression vector for generating mouse TREK-1

Depositor

Dan Minor

Publication

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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GPRC5D-DuET

Plasmid

#213298

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Publication

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GPRC5D (GPRC5D Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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Opto-ACTU−

Plasmid

#198869

Purpose

Optogenetic recruitment construct to increase inner membrane surface charge

Depositor

Peter Devreotes

Publication

Banerjee et al Nat Cell Biol. 2022 Oct;24(10):1499-1515

Insert

Rad17 polyanionic tail (-14)

Use

Synthetic Biology

Tags

CRY2PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+Lifeact-eGFP

Plasmid

#128428

Purpose

Contains insert allowing in-vivo visualization of F-actin

Depositor

Rusty Lansford

Publication

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

Lifeact-eGFP

Expression

Mammalian

Promoter

CMV & SP6

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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2xsspB-mSc-sspB-Tiam1

Plasmid

#114424

Purpose

Translocating Tiam1 to iLID and activate Rac1

Depositor

Dorus Gadella

Publication

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

Tiam1

Tags

mScarlet

Expression

Mammalian

Mutation

truncated Tiam1 (64-437)

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowY-kCAAX

Plasmid

#115564

Purpose

dark ITRACK acceptor construct for dual color imaging

Depositor

Ryohei Yasuda

Publication

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

ShadowY with KRas membrane targeting domain

Tags

ShadowY

Expression

Mammalian

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-P70a-Top-T~P70a-Bottom-T

Plasmid

#87314

Purpose

Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

Depositor

Donald Burke

Publication

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system

Use

Synthetic Biology

Expression

Bacterial

Promoter

P70a

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Smad5-3'UTR

Plasmid

#61777

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad5

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad5 3'UTR

Use

Luciferase

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only