We narrowed to 148,861 results for: ins;

1. pRS414-Fyv6-CFLAG

   Plasmid

   #234133

   Purpose

   Restriction digest from pUC18-CFLAG-Fyv6 and ligation into pRS414

   Depositor

   Aaron Hoskins

   Insert

   Fyv6
   

   Expression

   Yeast

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS414-Fyv6

   Plasmid

   #234140

   Purpose

   Amplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS414

   Depositor

   Aaron Hoskins

   Insert

   Fyv6
   

   Expression

   Yeast

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS414-NFLAG-Fyv6

   Plasmid

   #234139

   Purpose

   Restriction digest from pUC18-NFLAG-Fyv6 and ligation into pRS414

   Depositor

   Aaron Hoskins

   Insert

   Fyv6
   

   Expression

   Yeast

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS424 Prp8 G1868R

   Plasmid

   #234124

   Purpose

   Suppressor screen mutant in full-length Prp8 on a TRP plasmid

   Depositor

   Aaron Hoskins

   Insert

   Prp8
   

   Expression

   Yeast

   Mutation

   G1868R

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS416-Fyv6

   Plasmid

   #234141

   Purpose

   Amplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS416

   Depositor

   Aaron Hoskins

   Insert

   Fyv6
   

   Expression

   Yeast

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS424 Prp8 WT TRP1

   Plasmid

   #234120

   Purpose

   The N-terminus of Prp8 was restored thereby destroying the NheI site and correcting the ORF

   Depositor

   Aaron Hoskins

   Insert

   Prp8
   

   Expression

   Yeast

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Fyv6-NFLAG del1-51

   Plasmid

   #234136

   Purpose

   First 51 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminus

   Depositor

   Aaron Hoskins

   Insert

   Fyv6
   

   Expression

   Yeast

   Mutation

   N-terminus truncation

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Fyv6-NFLAG del1-16

   Plasmid

   #234138

   Purpose

   First 16 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminus

   Depositor

   Aaron Hoskins

   Insert

   Fyv6
   

   Expression

   Yeast

   Mutation

   N-terminus truncation

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Fyv6-NFLAG del1-23

   Plasmid

   #234137

   Purpose

   First 23 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminus

   Depositor

   Aaron Hoskins

   Insert

   Fyv6
   

   Expression

   Yeast

   Mutation

   N-terminus truncation

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET16b-Prp16

    Plasmid

    #111351

    Purpose

    protein purification

    Depositor

    Christine Guthrie

    Insert

    PRP16
    

    Tags

    N-terminally 10xHis-tagged proteins with a Factor…

    Expression

    Bacterial

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS414-Syf1 817 Truncation

    Plasmid

    #234126

    Purpose

    Syf1 PCR truncation starting at 817 through the end of the gene

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Expression

    Yeast

    Mutation

    N-terminus truncation

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS414-Syf1 634 Truncation

    Plasmid

    #234116

    Purpose

    Syf1 PCR truncation starting at 634 through the end of the gene

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Expression

    Yeast

    Mutation

    N-terminus truncation

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS414-Syf1 778 Truncation

    Plasmid

    #234115

    Purpose

    Syf1 PCR truncation starting at 778 through the end of the gene

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Expression

    Yeast

    Mutation

    N-terminus truncation

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1B-lambdaN-HA-DmMUB-E_AA

    Plasmid

    #148216

    Purpose

    Insect Expression of DmMUB-E

    Depositor

    Elisa Izaurralde

    Insert

    DmMUB-E
    

    Expression

    Insect

    Mutation

    two silent mtations and an insertion of 4 AA (91W…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. mAmetrine-CytERM-N-17

    Plasmid

    #56313

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 406, Emission: 526

    Depositor

    Michael Davidson

    Insert

    CytERM
    

    Tags

    mAmetrine

    Expression

    Mammalian

    Mutation

    Plasmid contains first 29 AA of Rabbit P450

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. Fyv6-NFLAG del103-173

    Plasmid

    #234134

    Purpose

    Amino acids 103-173 deleted in Fyv6 with FLAG epitope tag at N-terminus

    Depositor

    Aaron Hoskins

    Insert

    Fyv6
    

    Expression

    Yeast

    Mutation

    C-terminus truncation

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Fyv6-NFLAG del134-173

    Plasmid

    #234135

    Purpose

    Amino acids 134-173 deleted in Fyv6 with FLAG epitope tag at N-terminus

    Depositor

    Aaron Hoskins

    Insert

    Fyv6
    

    Expression

    Yeast

    Mutation

    C-terminus truncation

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-D1-IRES-SS-D2-IRES-HA-D3

    Plasmid

    #215119

    Purpose

    Expresses FLAG-Cyclin D1, SS-Cyclin D2 and HA-Cyclin D3 in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Inserts

    CCND1 (CCND1 Human)
    CCND2 (CCND2 Human)
    CCND3 (CCND3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-tag, HA-tag, and SS (STREP-STREP)

    Expression

    Mammalian

    Promoter

    IRES and TREG3

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

    Plasmid

    #118155

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hPGK and U6

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid

    #238007

    Purpose

    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.

    Depositor

    Agnel Sfeir, Simon Powell

    Article

    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:

    Insert

    AAVS1 (AAVS1, PPP1R12C Human)
    

    Use

    Rna donor to study rt-dsbr

    Expression

    Mammalian

    Mutation

    3bp insertion signature (ATC) at Cas9 cut site in…

    Promoter

    CMV

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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