We narrowed to 16,111 results for: form

1. Ncam1_1.0.1-Fc-His

   Plasmid

   #72087

   Purpose

   Expresses the extracellular region of the NCAM1, isoform 1.0.1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ncam1_1.0.1 Mouse (Ncam1 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pInducer20 CARD8-HA PAM S297A

   Plasmid

   #169983

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, S297A

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Gal4-entry

   Plasmid

   #128014

   Purpose

   Vector containing the Gal4-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   Gal4-DBD-3xFlag
   

   Use

   Gal4 entry vector

   Tags

   Gal4-DBD-3xFlag

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5FRT/TO PP1gamma-2*Strep

   Plasmid

   #113506

   Purpose

   Expresses dual Strep-tagged PP1gamma in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   PP1gamma (PPP1CC Human)
   

   Tags

   Strep-Strep

   Expression

   Mammalian

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p3E-DysF

   Plasmid

   #109544

   Purpose

   Multisite gateway vector for 3' tagging with Dysferlin

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Dysferlin (dysf Zebrafish)
   

   Use

   Zebrafish plasmids

   Mutation

   Please see Depositor Comments

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-myc-BirA*-NRASG12V

   Plasmid

   #122209

   Purpose

   Expresses myc-BirA* fused to NRASG12V in mammalian cells

   Depositor

   Christopher Counter

   Article

   Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

   Insert

   NRASG12v (NRAS Human)
   

   Tags

   myc-BirA*

   Expression

   Mammalian

   Mutation

   changed G12 to V

   Promoter

   CMV

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FB003

   Plasmid

   #119677

   Purpose

   Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   PtrpC:hph:Ttub
   

   Use

   Synthetic Biology

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBW2701_pCAG-PV1-iCre-N270-L1-nMag-NLS-BGHpA

   Plasmid

   #108823

   Purpose

   Expresses N-terminus to aa270 of Cre recombinase fused to nMag CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa270
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   nMag,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBW2702_pCAG-PV1-pMag-L1-iCre-271C-BGHpA

   Plasmid

   #108824

   Purpose

   Expresses aa271 to C-terminus of Cre recombinase fused to pMag CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment aa271 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS and pMag

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLIK-Venus-TmiR-G13-G12

    Plasmid

    #25742

    Purpose

    Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 13 and 12 miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti CRISPR V2 sgShmt2_1

    Plasmid

    #106314

    Purpose

    Express Cas9 and sgRNA targeting mShmt2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT2 (Shmt1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBW2662_pCAG-PV1-FKBP-L1-iCre-271C-NLS-BGHpA

    Plasmid

    #108738

    Purpose

    Expresses aa271 to C-terminus of Cre recombinase fused to FKBP CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa271 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    FKBP and NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDB.His.MBP.3C NLRP1 UPA-CARD WT

    Plasmid

    #164003

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBW2583_pCAG-PV1-VCre-N172-L1-ABI-NLS-BGHpA

    Plasmid

    #108783

    Purpose

    Expresses N-terminus to aa172 of VCre recombinase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment N-terminus to aa172
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBW2587_pCAG-PV1-PYL-L1-VCre-173C-NLS-BGHpA

    Plasmid

    #108784

    Purpose

    Expresses aa173 to C-terminus of VCre recombinase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment aa173 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSD35_pCAG-PV1-TP901-N326-L1-FRB-NLS-BGHpA

    Plasmid

    #108813

    Purpose

    Expresses N-terminus to aa326 of TP901 integrase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    TP901 integrase fragment N-terminus to aa326
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBW1602_pCAG-PV1-PhiC31-N428-L1-ABI-NLS-BGHpA

    Plasmid

    #108801

    Purpose

    Expresses N-terminus to aa428 of PhiC31 integrase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment N-terminus to aa428
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBW1607_pCAG-PV1-PYL-L1-PhiC31-429C-BGHpA

    Plasmid

    #108802

    Purpose

    Expresses aa429 to C-terminus of PhiC31 integrase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment aa429 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBW2278_pCAG-PV1-FlpO-N168-L1-ABI-NLS-BGHpA

    Plasmid

    #108765

    Purpose

    Expresses N-terminus to aa168 of Flp recombinase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment N-terminus to aa168
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBW2279_pCAG-PV1-PYL-L1-FlpO-169C-NLS-BGHpA

    Plasmid

    #108766

    Purpose

    Expresses aa169 to C-terminus of Flp recombinase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment aa169 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBW2211_pCAG-PV1-iCre-N256-L1-GID1-NLS-BGHpA

    Plasmid

    #108727

    Purpose

    Expresses N-terminus to aa256 of Cre recombinase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment N-terminus to aa256
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBW2231_pCAG-PV1-GAI-L1-iCre-257C-NLS-BGHpA

    Plasmid

    #108728

    Purpose

    Expresses aa257 to C-terminus of Cre recombinase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa257 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX4T-1 hSlp4-a del.SHD

    Plasmid

    #40067

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a V21A (SYTL4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted SHD domain (1-143)

    Promoter

    Tac

    Available Since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pCMV5-Flag-EPLIN beta delta C term

    Plasmid

    #40931

    Depositor

    Scott Maul, Elizabeth Luna

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN beta (LIMA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C terminus deleted

    Available Since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pVE2_3_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_11xQUAS

    Plasmid

    #154223

    Purpose

    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 11xQUAS+CP_pcbC

    Depositor

    Arnold Driessen

    Article

    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

    Insert

    p40S:QfDBD-VP16AD-GFP-NLS
    

    Use

    Synthetic Biology; Fungal expression

    Tags

    GFP-NLS; DsRed-SKL

    Expression

    Bacterial

    Promoter

    p40S (AN0465 A. nidulans)

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLM2_42_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS

    Plasmid

    #154238

    Purpose

    Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbC

    Depositor

    Arnold Driessen

    Article

    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

    Insert

    pgndA:QfDBD-VP16AD-GFP-NLS
    

    Use

    Synthetic Biology; Fungal expression

    Tags

    GFP-NLS; DsRed-SKL

    Expression

    Bacterial

    Promoter

    pGndA (An11g02040 A. niger)

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDEST-CMV GFP-GABARAPL1 G116A

    Plasmid

    #123118

    Purpose

    Expresses EGFP-GABARAPL1 G116A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Glycine 116 to Alanine

    Promoter

    CMV

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Nrp2.5-Fc-His

    Plasmid

    #72102

    Purpose

    Expresses the extracellular region of the Neuropilin 2, isoform 5 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Nrp2.5 (Nrp2 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDB.His.MBP.3C NLRP1 UPA WT

    Plasmid

    #164010

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Available Since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. actc1b-mKate2-ARF1-DN

    Plasmid

    #109536

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to dominant negative ARF1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ARF1 (arf1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Mutation

    DN

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBW2689_pCAG-PV1-iCre-N229-L1-nMag-NLS-BGHpA

    Plasmid

    #108817

    Purpose

    Expresses N-terminus to aa229 of Cre recombinase fused to nMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment N-terminus to aa229
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    nMag,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBW2690_pCAG-PV1-pMag-L1-iCre-230C-BGHpA

    Plasmid

    #108818

    Purpose

    Expresses aa230 to C-terminus of Cre recombinase fused to pMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa230 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and pMag

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBW2210_pCAG-PV1-iCre-N251-L1-GID1-NLS-BGHpA

    Plasmid

    #108725

    Purpose

    Expresses N-terminus to aa251 of Cre recombinase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment N-terminus to aa251
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDF1-PTPRD-P459L

    Plasmid

    #25644

    Depositor

    Todd Waldman

    Article

    Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

    Insert

    Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    tumor-derived mutation created by site-directed m…

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

35. G protein alpha-o-R179C-EE

    Plasmid

    #55780

    Purpose

    This is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    G protein alpha-o with constitutively activating R179C mutation (Gnao1 Rat)
    

    Tags

    EE epitope was introduced internally with D167E a…

    Expression

    Bacterial and Mammalian

    Mutation

    Arginine 179 was replaced with cysteine

    Promoter

    CMV

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Nrp2.1-AP-His

    Plasmid

    #71972

    Purpose

    Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Nrp2.1 (Nrp2 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p3E-Clta

    Plasmid

    #109585

    Purpose

    Multisite gateway vector for 3' tagging with Clathrin light chain a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Clathrin light chain a (clta Zebrafish)
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBW2121_pCAG-PV1-Bxb1-N468-L1-GID1-NLS-BGHpA

    Plasmid

    #108815

    Purpose

    Expresses N-terminus to aa468 of Bxb1 integrase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Bxb1 integrase fragment N-terminus to aa468
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. JJ802: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold

    Plasmid

    #121812

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. gRNA[Sxl].1026B

    Plasmid

    #112688

    Purpose

    express gRNA targeting Sxl under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[Sxl] (Sxl Fly)
    

    Use

    CRISPR

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. actc1b-mKate2-ARF6

    Plasmid

    #109537

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to ARF6

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ARF6 (arf6a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. actc1b-mKate2-ARF6-DN

    Plasmid

    #109538

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to dominant negative ARF6

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ARF6 (arf6a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Mutation

    DN

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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43. pBW2678_pCAG-PV1-pMag-L1-FlpO-397C-NLS-BGHpA

    Plasmid

    #108832

    Purpose

    Expresses aa397 to C-terminus of Flp recombinase fused to pMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment aa397 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and pMag

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Dscam1_7.27.25 EC10-Fc-His

    Plasmid

    #72188

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.25 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.25
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Dscam1_7.27.25 EC10-AP-His

    Plasmid

    #72062

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.25 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.25
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBlue-hFX-HA

    Plasmid

    #14987

    Depositor

    Michael Ristow

    Article

    Shoichet et al Hum Mol Genet. 2002 Apr 1. 11(7):815-21.

    Insert

    human frataxin cDNA (FXN Human)
    

    Tags

    AU1 and HA

    Expression

    Bacterial

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

47. pBW2585_pCAG-PV1-VCre-N269-L1-ABI-NLS-BGHpA

    Plasmid

    #108785

    Purpose

    Expresses N-terminus to aa269 of VCre recombinase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment N-terminus to aa269
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBW2589_pCAG-PV1-PYL-L1-VCre-270C-NLS-BGHpA

    Plasmid

    #108786

    Purpose

    Expresses aa270 to C-terminus of VCre recombinase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment aa270 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pDONR223 LC3B G120

    Plasmid

    #123074

    Purpose

    Gateway entry clone encoding human MAP1LC3B G120

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Deleted amino acids 121-125. Stop codon after G12…

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pDONR223 GABARAPL1 G116

    Plasmid

    #123085

    Purpose

    Gateway entry clone encoding human GABARAPL1 G116

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Deleted amino acid 117. Stop codon after G116

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge