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pNCST-AausFP3

Plasmid

#129501

Purpose

Expresses AausFP3 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP3

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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MTK1_023

Plasmid

#123685

Purpose

Encodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L1- B1 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_024

Plasmid

#123686

Purpose

Encodes ConL2 and human C3 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L2-C3 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_001

Plasmid

#123697

Purpose

Encodes pTRE as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pTRE

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_006

Plasmid

#123701

Purpose

Encodes pSV40 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSV40

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB1975 (pUC19-PTEF1-PPGK1)

Plasmid

#73296

Purpose

Example plasmid containing a bidirectional promoter

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

pTEF1-pPGK1

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PML-ER-pBabe

Plasmid

#73556

Purpose

vector encoding a fusion protein between PML-IV and ER (estrogen receptor)

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

PML-IV (PML Human)

Use

Retroviral

Tags

ER

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF138-delta 18-58

Plasmid

#78921

Purpose

Mammalian expression of RNF138 with delta 18-58 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Tags

EGFP

Expression

Mammalian

Mutation

delta 18-58

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Luc-Nanog-3'UTR

Plasmid

#63893

Purpose

Luciferase reporter of mouse wildtype Nanog 3'UTR

Depositor

Lin He

Article

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

Nanog homeobox (Nanog Mouse)

Expression

Mammalian

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pSuper.Retro.Puro-PKN1 shRNA 4

Plasmid

#59978

Purpose

Knockdown of PKN1

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

PKN1 shRNA

Use

Retroviral

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCDF1-GRM3 (E870K)

Plasmid

#31802

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

Insert

GRM3 (GRM3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed glutamic acid 870 to lysine

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-GRM3 (S610L)

Plasmid

#31800

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

Insert

GRM3 (GRM3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed serine 610 to leucine

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
AAV-CAG-FLPX-rc [Chronos-tdTomato]

Plasmid

#105834

Purpose

AAV-mediated expression of Chronos-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-tdTomato

Use

AAV

Tags

tdTomato (codon diversified version)

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Chronos-GFP

Plasmid

#99232

Purpose

AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB2904(XI-3 MarkerFree)

Plasmid

#73276

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pQUAST-M13::GAL4DBDo

Plasmid

#64724

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

M13::GAL4DBDo

Expression

Insect

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only

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pRM430

Plasmid

#44530

Depositor

Michael Grunstein

Article

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHT2

Expression

Yeast

Mutation

hht2 deletion 4-30

Available Since

June 4, 2013

Availability

Academic Institutions and Nonprofits only
pUK192

Plasmid

#44962

Depositor

Michael Grunstein

Article

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

LEU2

Expression

Yeast

Mutation

HHTI-HHF1 is replaced with LEU2

Available Since

June 4, 2013

Availability

Academic Institutions and Nonprofits only
pRM214

Plasmid

#44527

Depositor

Michael Grunstein

Article

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHT2

Expression

Yeast

Mutation

K14R

Available Since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
pRM204

Plasmid

#44528

Depositor

Michael Grunstein

Article

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHF2-HHT2

Expression

Yeast

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only