We narrowed to 4,188 results for: Pet2

1. SpyTag003-mKate2

   Plasmid

   #133452

   Purpose

   Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-mKate2
   

   Use

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyTag003-sfGFP

   Plasmid

   #133454

   Purpose

   Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-sfGFP
   

   Use

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpyCatcher003-mi3

   Plasmid

   #159995

   Purpose

   Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyCatcher003-mi3
   

   Use

   Tags

   C-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. LM627

   Plasmid

   #191551

   Purpose

   LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

   Depositor

   David Baker

   Article

   Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

   Insert

   ccdb
   

   Use

   Tags

   SNAC-tag, HIS-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LM670

   Plasmid

   #191552

   Purpose

   LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

   Depositor

   David Baker

   Article

   Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

   Insert

   ccdb
   

   Use

   Tags

   HIS-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJBL715

   Plasmid

   #140385

   Purpose

   Expresses MphR protein under the control of T7-lacO promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-lacO-MphR-T
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJBL723

   Plasmid

   #140393

   Purpose

   Expresses HucR protein under the control of T7-lacO promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-lacO-HucR-T
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJBL713

   Plasmid

   #140383

   Purpose

   Expresses CtcS protein under the control of T7-lacO promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-lacO-CtcS-T
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FnR9-10

   Plasmid

   #182567

   Purpose

   Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 9-10
   

   Use

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1379A

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ecoli cpGFP

    Plasmid

    #215713

    Purpose

    Expresses cpGFP in Ecoli

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    cpGFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    circularly permuted GFP

    Promoter

    T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Human USP30 (64-502, construct 8, MG-26-21)

    Plasmid

    #110745

    Purpose

    Bacterial expression of human USP30 (construct 8)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    N-His6-3C-GST-3C

    Expression

    Bacterial

    Mutation

    F348D, M350D, I353E + insertion deletion: 64-357;…

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. RpHLuorin2

    Plasmid

    #180227

    Purpose

    for bacterial expression of recombinant ratiometric pHluorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2
    

    Use

    Tags

    His-tag followed by thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETNb-cALFA

    Plasmid

    #159989

    Purpose

    For the protein expression with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-21a(+)-IPP1-His

    Plasmid

    #118978

    Purpose

    Bacterial expression plasmid for Inorganic pyrophosphatase

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    inorganic diphosphatase IPP1 (IPP1 Budding Yeast)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. S48I48 ELP

    Plasmid

    #68394

    Purpose

    Elastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residue

    Depositor

    Andrew MacKay

    Article

    Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

    Insert

    S48I48 ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOlyA(WT)

    Plasmid

    #132391

    Purpose

    Ostreolysin A for sensing sphingomyelin/cholesterol complexes

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A wild-type
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, C94S, S151C

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDR119

    Plasmid

    #13332

    Depositor

    Martin Gellert

    Article

    Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

    Insert

    DNA ligase IV (LIG4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

18. HECTD1

    Plasmid

    #32860

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HECTD1 (HECTD1 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 1879-1966 only

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

19. SARS-CoV-2 Mpro protease (M165I mutant - clinical mutant); aliases: 3CLpro, 3C-like

    Plasmid

    #204778

    Purpose

    Bacterial expression of Sars Cov 2 MPRO M165I mutant

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M165I

    Promoter

    Available since

    Dec. 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pBJG1 T7-8xHis-OGT

    Plasmid

    #154271

    Purpose

    Bacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

    Depositor

    Suzanne Walker

    Article

    Gross et al J Am Chem Soc. 2005 Oct 26;127(42):14588

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Tags

    8x His, HRV3C protease site, and T7 leader

    Expression

    Bacterial

    Mutation

    bacterial codon optimized

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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