We narrowed to 21,797 results for: Sis

1. pcDNA-flag-mKdm4d-polyA

   Plasmid

   #61553

   Purpose

   For mRNA synthesis or expression in mammalian cells

   Depositor

   Yi Zhang

   Article

   Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

   Insert

   Kdm4d (Kdm4d Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPML1 (GB0045)

   Plasmid

   #68164

   Purpose

   Provides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtML1 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXSE901BG

   Plasmid

   #91714

   Purpose

   CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMBA333

   Plasmid

   #214745

   Purpose

   Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23119-RiboJ-RBS(21992)-gfpmut3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO DNAJB1

   Plasmid

   #19468

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   DNAJB1 (DNAJB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRelA'

   Plasmid

   #175595

   Purpose

   Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   Catalytic domain of E.coli relA gene.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Only the catalytic domain of the enzyme is used (…

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWaldo-GFPe_PepTSo

   Plasmid

   #58334

   Purpose

   Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

   Depositor

   Simon Newstead, So Iwata

   Article

   Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

   Insert

   PepTso
   

   Use

   Tags

   GFP-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pHDE-35S-Cas9-mCherry

   Plasmid

   #78931

   Purpose

   For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants

   Depositor

   Yunde Zhao

   Article

   Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. OSCA2.3

   Plasmid

   #136595

   Purpose

   Expresses the mechanosensitive channel OSCA2.3 in mammalian cells

   Depositor

   Ardem Patapoutian

   Article

   Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

   Insert

   OSCA2.3 (HYP1 Mustard Weed)
   

   Use

   Tags

   pIRES2-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYFAC-CH4

    Plasmid

    #168980

    Purpose

    AMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in asp…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYFAC-CH2

    Plasmid

    #168978

    Purpose

    AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in asp…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUB-spHRed

    Plasmid

    #84734

    Purpose

    Expresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stoma

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    pHRed
    

    Use

    Tags

    RbcS targeting sequence

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xFLAG-dCas9/pMSCVpuro

    Plasmid

    #134983

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIRES-Ap1g1-FKBP

    Plasmid

    #46945

    Purpose

    Co-expresses siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cells

    Depositor

    Margaret Robinson

    Article

    Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

    Insert

    Ap1g1-FKBP
    

    Use

    Tags

    Mainly mouse Ap1g1 cDNA, with a fragment of brain…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAP03-ermE*p

    Plasmid

    #120234

    Purpose

    Carries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    promoter cassette containing ermE*p promoter and ampicillin resistance gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    bla (amp resistance gene)

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7C-BAG2

    Plasmid

    #177654

    Purpose

    Recombinant protein synthesis of human BAG2

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    BAG2 (BAG2 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

    Plasmid

    #124861

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. peCALWY-1

    Plasmid

    #22233

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-1 Zn(II) sensor (ATOX1 Human, GFP's)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    linker consists of 9 GGSGGS repeats

    Promoter

    Available since

    Nov. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

19. hHSF4b WT

    Plasmid

    #118356

    Purpose

    This plasmid expresses human HSF4b WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGMCtN-TSC10M1-sspB

    Plasmid

    #49521

    Purpose

    Nourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    TSC10M1-sspB
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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